STATISTICAL OPTIMIZATION OF MEDIUM COMPONENTS BY PLACKETT BURMAN DESIGN AND RESPONSE SURFACE METHODOLOGY FOR ENHANCED ANTIOXIDANT ACTIVITY BY XYLARIA FEEJEENSIS HMJAU22039
DOI:
https://doi.org/10.22159/ijpps.2016v8i11.14257Keywords:
DPPH, TPC, Endophytic fungi, Central composite designAbstract
Objective: To enhance the antioxidant potential of Xylaria feejeensis by statistical optimization. The components of potato dextrose yeast extract (PDYEB) medium: potato infusion, dextrose and yeast extract were investigated to optimize antioxidant activity by Plackett-Burman design (P-B design). The interaction between the critical components of the medium and incubation time was further investigated by Response Surface Method (RSM) and the culture conditions were optimized for enhancing antioxidant activity. Central composite design (CCD) was employed and quadratic response surface model was used to analyze the data using analysis of variance (ANOVA).
Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay, nitric oxide (NO) scavenging assay. The antioxidant activity was compared with total phenolic content measured by Folin-Ciocalteau (FC) reagent based assay.
Results: Plackett-Burman design revealed dextrose and yeast extract to be the most signiï¬cant components of PDYEB medium (p<.0001). ANOVA analysis showed that the model was highly significant (p<0.0001) for antioxidant activity measured by DPPH, NO scavenging and TPC (total phenolic content). The model was justified by applying the optimized conditions and values of 87.38%, 56.43%, 27.28 mg/g for DPPH, NO scavenging and TPC respectively were obtained. The estimated optimum conditions of the variables for the antioxidant activity and phenolic content are 12.72g of dextrose, 1.67g yeast extract, and incubation period of 23.43 d.
Conclusion: The study highlights the importance of various components of the medium and the physiochemical limitations in antioxidant potential and phenol production of the fungal strain.Â
Downloads
References
Santhosh Goveas W, Asha Abraham. Evaluation of antimicrobial and antioxidant activity of stem and leaf extracts of Coscinium fenestratum. Asian J Pharm Clin Res 2013;6:218-21.
Pracheta, Veena Sharma, Ritu Paliwal, Sadhana Sharma. In vitro free radical scavenging and antioxidant potential of ethanolic extract of Euphorbia neriifolia Linn. Int J Pharm Pharm Sci 2011;3:238Â42.
Robin Sharma, Rashmi R, Vijaya Kumar BS. Application of plackett-burman design to evaluate media components affecting antioxidant activity of an endophytic fungus Penicillium sp. isolated from the bark of Aegle marmelos. Int J Pharm Biol Sci 2014;5:1010–9.
Swati Hegde, Gururaj Bhadri, Kavita Narsapur, Shanta Koppal. Statistical optimization of medium components by response surface methodology for the enhanced production of bacterial cellulose by Gluconacetobacter persimmonis. J Bioprocess Biotech 2013;4:1.
Rahayu Ahmad, Najeeb Kaid Nasser Al-Shorgani, Aidil Abdul Hamid. Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by Lentinus squarrosulus. Adv Biosci Biotechnol 2013;4:1079-85.
Zhao, Xiang GR, Ye ZJ. Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chem 2006;99:767-74.
Kang KS, Yokozawa T, Kim HY. Study on the nitric oxide scavenging effects of Ginseng and its compound. J Agric Food Chem 2006;54:2558-62.
Singleton VL, Ortofehr R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrate and antioxidants by means of folin-ciocalteau reagent. Methods Enzymol 1999;299:152-78.
Miao LI, Kwong TFN, Qian PY. Effect of culture conditions on mycelial growth, antibacterial activity, and metabolite proï¬les of the marine-derived fungus Arthrinium c. f. saccharicola. Appl Microbiol Biotechnol 2006;72:1063–73.
Gogoi DK, Deka Boruah HP, Saikia R, Bora TC. Optimization of process parameters for improved production of the bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India. World J Microbiol Biotechnol 2008;24:79–87.
Zhao GR, Xiang ZJ, Ye TX, Yuan YJ, Guo ZX. Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chem 2006;99:767–74.
Huang WY, Cai YZ, Hyde KD, Corke H, Sun M. Endophytic fungi from Nerium oleander L (Apocynaceae): main constituents and antioxidant activity. World J Microbiol Biotechnol 2007;23:1253–63.
Published
How to Cite
Issue
Section
