ANTIMICROBIAL POTENTIALOF HEMOLYMPH AND HEPATOPANCREAS OFPORTUNUSSEGNISCRABS

Authors

  • Mona Hajirasouli Faculty of Biological Science, Shahid Beheshti University G. C., P. O. Tehran, Iran.
  • Jamileh Pazooki Faculty of Biological Science, Shahid Beheshti University G. C., P. O. Tehran, Iran.

Keywords:

Crab, Hemolymph, Hepatopancreas, Antimicrobial

Abstract

Objective: The potential of marine crabs as a source of bioactive products is largely unexplored.

Methods: Portunussegnis crabs were collected from the northern coast of Persian Gulf. Antimicrobial activities of hepatopancreas,hemolymph and proteinsof hemplymphfrom P. segniscrabs against a range of 11 different bacterial and 6 fungal pathogen strains were examined. Two positive controls Amoxicillin and Penicillin were also used.

Results: The maximum(16 mm) and minimum (7.2 mm) activity was recorded frommale hemolymph and male hepatopancreas against Entrobacter sp. and Serratiamarcesences respectively. Incomparison with bacterial pathogens, fungal pathogens showed more resistance to the extracts.

Conclusion: Results show that hemolymph and hepatopancreas of brachyuran crabs may have Antimicrobial activity.

Downloads

Download data is not yet available.

References

Ravichandran, SS Wahidulla, L D Souza, G Rameshkumar. Antimicrobial lipids from the hemolymph of brachyuran crabs. J Appl Biochem Biotechnol 2010;162(4):1039-51.

Smith VJ, JR Chisholm. Antimicrobial protein in crustaceans. J Adv Exp Med Biol 2001;484:95-112.

Rameshkurmar, GS Ravichandran, G Kaliyavarathan, TT Ajithkumar. Antimicrobial peptide from the crab, Thalamitacrenata (Latreille, 1829). World J of Fish and Marine Sci 2009;1(2):74-9.

Stewart JE, BM Zwicker. Natural and induced bactericidal activities in the hemolymph of the lobster; Homarus americanus: products of hemocyte-plasma interactions. Canadian J of Microbiology 1972;18:1499-509.

Rameshkumar, GT Aravindhan, S Ravichandran. Antimicrobial proteins from the crab Chrybdislucifera (Fabricius, 1798). Middle-East J of Scientific Res 2009;4(1):40-3.

Miyata T, F Tokunaga, T Yoneya, K Yoshikawa, S Iwanaga, M Niwa, T Takao, Y. Shimonishi. Antimicrobial peptides, isolated from horseshoe crab hemocytes, Tachyplesinm II and PolyphemisinsI and II:chemical structures and biological activity. J of Biochemistry 1989;106:663-8.

Veeruraj, A S Ravichandran, G Rameshkumar. Antibacterial activity of crab hemolymph on clinical pathogens. J Trend in Applied Sci Res 2008;3:174-81.

Anitha A, K Sharath. Antimicrobial peptides from the haemolymph of crabs Scylla serrata and Metapograpsusmessor. Asiatic J of Biotechnology Resources 2(5):568-76.

Chodsang S, S Benjakul, W Visessanguan, K Hara, A Yoshida, X Liang. Low molecular weight trypsin from hepatopancreas of freshwater prawn(Macrobrachiumrosenbergii):characteristics and biochemical properties. J Food Chemistry 2012;134:351-8.

Baure AW, WMM Kirby, MJC Sherris, M Turck. Antibiotic susceptibility testing by a standardized single disc method. American J of Clinical Patbol 1996;45:493-49.

Edward N J, T A Arroll, Z Fan. Specificity some physiochemical characteristics of antibacterial activity from blue crabsCallinectessapidus. J Fish Shellfish Immunol 1996;6:403-12.

Hoq M I, M U Seraj, S Chowdhury. Isolation and characterization of antibacterial peptides from the mud crab Scylla serrata. Pakisthan J of Biological Sci 2003;6(11):1345-53.

Schnapp D, GD Kemp, VJ Smith. Purification and characterization of a prolin rich antibacterial peptide, with sequence similarity to bactenecin-7, from the hemocytes of the shore crab, Carcinusmaenas. European J of Biochemistry 1996;240:532-39.

Anbuchezhian RM, S Ravichandran, GRameshkumar, T T Ajithkumar. Influence of crab haemolymph on clinical pathogens. Adv in Biological Res 2009;3(3-4):104-9.

Kumaravel K, Ravichandran S, Sritama BS. In vitro antimicrobial activity of shrimpshaemolymph on clinical pathogens. African J of Microbiology Res 2010;4(23):2592-6.

Nakamura T, Furanaka H, Miyata T, Muta F, Iwanaga S, Niw M, Takao T, Shimonishi, Y. 1988. Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshore crab (Tachypleustridentatus). J Biol Chem 263:16709-13.

Gudmundsson CG, Lidholh DA, Asling B, Gan R, Boman HG. Thececropin locus:cloning and expression of a gene cluster encoding three antibacterial peptides in Hyalophoracecropia. J Biol Chem 1991;266:11510-17.

Chisholm JRS, Smith VJ. Antibacterial activity in the hemocytes of the shore crab, Carcinusmaenas. J Mar Biol Ass UK 1992;72:529-42.

Destoumieux D, Bulet P, Loew D, Dorsselaer V A, Rodriguea J, Bachere E. Penaedine, a new family or antimicrobial peptides isolated from the shrimp penaeusvannamei(Decapoda). J Biol Chem 1997;72:28398-406.

Ravichandran S, Jeyalakshmi S, Sudha S, Anbuchezhian R. Antimicrobial peptides from the haemolymph of the prawn Macrobrachiumrosenbergii. J of the Bangladesh Pharm Society (BDPS) 2010;5:6267.

Noga EJ, Arroll TW, Fan Z. Specificity and some physiochemical characteristics of the antibacterial activity from the blue crabs Callinectessapidus. Fish Shellfish Immunol 1996;6(6):403-12.

Cuthbertson BJ, Bullesbach EE, Gross PS. Discovery of synthetic penaeidin activity against antibiotic resistant. Chem Biol Drug Des 2006;68:120-7.

Kawababa SI, Nagayama R, Hirata M, Shigenaga T, Agarwala KL, Saito T, et al. Tachycitin, a small granular component in horseshore crab hemocytes is an antimicrobial protein with chitin-binding activity. J Biochem 1996;120:1253-60.

Ravichandran S, Rameshkumar G, Anthonysa y A, Kumaravel K. Antibacterial activity from the haemolymph of some Brachyuran crabs. American-Eurasian J of Scientific Res 2009;4(1):26-33.

Published

31-08-2014

How to Cite

Hajirasouli, M., and J. Pazooki. “ANTIMICROBIAL POTENTIALOF HEMOLYMPH AND HEPATOPANCREAS OFPORTUNUSSEGNISCRABS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 601-3, https://journals.innovareacademics.in/index.php/ijpps/article/view/2765.

Issue

Section

Original Article(s)