ANTIOXIDANT ACTIVITY AND ANTIBACTERIAL SCREENING OF TUBERS OF AMORPHOPHALLUS KONKANENSIS AND AMORPHOPHALLUS BULBIFER (ARACEAE)
Keywords:
Antioxidant, Antibacterial, Phenolics, HPLC, Amorphophallus konkanensis, Amorphophallus bulbiferAbstract
Objective: Antioxidant activity (AOA) and antibacterial screening of tubers of unexplored Amorphophallus konkanensis Hett., Yadav & Patil (AKT) and Amorphophallus bulbifer (Roxb.) Bl. (ABT).
Methods: Antioxidant activity was evaluated by using ferric reducing antioxidant power (FRAP), 1 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl (OH•) radical, nitric oxide (NO∙) radical scavenging activities. Phenolics were determined by total phenolic content (TPC) assay. Antibacterial screening of different solvent extracts of tubers was tested against Gram positive and Gram negative bacterial strains by using the agar diffusion method. HPLC analysis was carried out for active phenolic compounds.
Results: Tubers were extracted with three different solvents (ethanol, acetone and water). Acetone extracts of AKT and ABT exhibited the highest phenolic content 29.37±0.83 and 20.62±1.04 mg GAE/g. The AKT and ABT acetone extracts possesses highest free radical scavenging activity towards FRAP (0.632 & 0.586 O. D), DPPH (85.78% & 82.68%), hydroxyl (OH∙) radical (82.43% & 80.26%), nitric oxide (NO∙) radical (81.34% & 80.8%). The phenolic compounds tannic acid, gallic acid, quercetin, p-coumaric acid, catechin were identified by the HPLC method in acetone extracts. Among the different extracts, acetone extract of both the species of Amorphophallus showed significant antibacterial activity against all Gram positive and Gram negative bacterial strains tested.
Conclusion: The results indicated that AKT and ABT can be considered as good sources of natural antioxidant for medicinal, commercial, nutraceutical and functional food applications. Antibacterial efficacy shown by these plants provides a scientific basis for their traditional uses in remedies.
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References
Halliwell B. Free Radicals, Antioxidants and human disease: curiosity, cause, or consequence? Lancet 1994;344:721-4.
Chen K, Geoff W, Richard NB, Youngping B. Antioxidant activities of extracts from five anti-viral medicinal plants. J Ethnopharmacol 2005;96:201-5.
Halliwell B. Antioxidants and human diseases: a general introduction. Nutr Rev 1997;55:44-52.
Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem 2003;83:547–50.
How YL, Yau YL. Evaluation of antioxidant activities of the methanolic extracts of selected ferns in malaysia. Int J Env Sci Dev 2011;2(6):442-7.
Roy S, Dutta CM, Paul SB. Antibacterial activity of Araceae: an overview. Int J Res Ayur and Pharm 2013;4(1):15-7.
Umamaheswari A, Shreevidya R, Nuni A. In vitro Antibacterial activity of Bougainvillea spectabilis leaves extracts. Advan Biol Res 2008;2:01-05.
Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH. Natural plant chemicals: Sources of industrial and medicinal. Mater Sci 1985;228:1154-60.
Ikegami F, Fujii Y, Ishihara K, Satoh T. Toxicological aspects of Kampo medicines in clinical use. Chem Biol Int 2003;145:235-50.
Izzo AA. Drug interactions with St. John’s Wort (Hypericum perforatum): A review of the clinical evidence. Int J Clin Pharmacol Ther 2004;42:139-48.
Angayarkanni J, Ramkumar KM, Poornima T, Priyadarshini U. Cytotoxic activity of Amorphophallus paeoniifolius tuber extracts in vitro. Am Eurasian J Agric Environ Sci 2007;2(4):395–9.
Yusuf M, Chowdhury JU, Yahab MA, Begum J. Medicinal plants of Bangladesh, BCSIR Laboratories, Bangladesh; 1994. p. 2.
Chopra RN, Chopra IC, Handa KL, Kapur LD. Indigenous Drugs of India (2nd Edition), U. N. Dhur and Sons Private Ltd., 15, Bankim Chattrjee street, Calcutta; 1958.
Nataraj HN, Murthy RLN, Ramachandra SS. In vitro quantification of flavonoids and phenolic content of suran. Int J Chem Tech Res 2009;1:1063-7.
Yadu ND, Ajoy KG. Pharmacognostic evaluation and phytochemical analysis of the tuber of Amorphophallus paeoniifolius. Int J Pharm Res Dev 2010;2(9):44–9.
Sule A, Ahmed QU, Samah OA, Omar MN. Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria. J Med Plants Res 2011;5(1):7-14.
Jagtap UB, Panaskar SN, Bapat VA. Evaluation of antioxidant capacity and phenol content in Jackfruit (Artocarpus heterophyllus Lam.) fruit pulp. Plant Foods Hum Nutr 2010;65:99-104.
Ekrem K, Ercan B, Emrah D, Fatih T, Ilhami G. Antioxidant activity of Melissa officinalis leaves. J Med Plants Res 2011;5(2):217-22.
Lee HC, Kim JH, Jeong SM, Kim DR, Ha JU, Nam KC. Effect of far infrared radiation on the antioxidant activity of rice hulls. J Agric Food Chem 2003;51(15):4400–3.
Murthy KN, Chidambara, Rajasekaran T, Giridhar P, Ravishankar GA. Antioxidant property of Decalepis hamiltonii Wight and Arn. Indian J Exp Biol 2006;44:832-37.
Marcocci L, Packer L. Antioxidant assay of Ginkgo biloba extract EGB 761. Methods Enzymol 1994;234:462-75.
Kaur GJ, Arora DS. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Alternat Med 2009;9:30-4.
De S, Dey YN, Ghosh AK. Phytochemical investigation and chromatographic evaluation of the different extracts of tuber of Amorphophallus paeonifolius (Araceae). Int J Pharm Biomed Res 2010;1(5):150-7.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Nirali Prakashan 2009;6(6):16-7.
Biswas Nirupam, Balac Pauline, Narlakanti Sai Kishore, Haque MD Enamul, Hassan MD Mehedi. Identification of phenolic compounds in processed cranberries by HPLC method. J Nutr Food Sci 2013;3(1):1-5.
Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agri Food Chem 1999;47(10):3954–62.
Zhou K, Yu L. Antioxidant properties of bran extracts from Trego wheat grown at different locations. J Agri Food Chem 2004;52:1112–7.
Biglari F, Alkarkhi AFM, Easa AM. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Food Chem 2008;107:1636-41.
Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J Agric Food Chem 2002;50:2454-8.
Dorman HJ, Bachmayer O, Kosar M, Hiltunen R. Antioxidant properties of aqueous extracts from selected Lamiaceae species grown in Turkey. J Agric Food Chem 2004;52:762-70.
Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 2004;85:611-6.
Ozcelik C, Lee JH, Min DB. Effects of light, oxygen and pH on the absorbance of 2, 2-diphenyl-1-picrylhydrazyl. J Food Sci 2003;68:487–90.
Chourasiya RK, Jain PK, Jain SK, Nayak SS, Agrawal RK. In-vitro antioxidant activity of Clerodendron inerme Gaertn leaves. Res J Pharm Biol Chem Sci 2010;1:119-23.
Nagulendran KR, Velavan S, Mahesh R, Hazeena BV. In Vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. Electron J Chem 2007;4(3):440-9.
Sultana B, Anwar F, Pirzybyiski R. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica and Euginea jambolana Lam. Trees Food Chem 2007;104:1106-14.
Krishna KT, Ranjini CE, Sasidharan VK. Antibacterial and antifungal activity of secondary metabolities from some medicinal and other common plant species. J Life Sci 1997;2:14-9.
Natarajan E, Senthilkumar S, Francis Xavier T, Kalaiselvi V. Antibacterial activities of leaf extracts of Alangium salviifolium. J Trop Med Plants 2003;4:9-13.
Bauer R, Tittel G. Quality assessment of herbal preparations as a precondition of pharmacological and clinical studies. Phytomed 1996;2:193-8.
Springfield EP, Eagles PKF, Scott G. Quality assessment of South African herbal medicines by means of HPLC fingerprinting. J Ethnopharmacol 2005;101:75-83.
Singh S, Srivastava R, Choudhary S. Antifungal and HPLC analysis of the crude extracts of Acorus calamus, Tinospora cordifolia and Celestrus paniculatus. J Agric Tech 2010;6(1):149-58.
Jay JM. Modern Food Microbiology. 5th Ed. Chapman and Hall: New York; 1996.