STUDY OF IN VITRO ANTI-INFLAMMATORY ACTIVITY OF ETHNOMEDICINAL PLANTS OF SIKKIM VISCUM ARTICULATUM AND ACORUS CALAMUS
Objective: The present study was carried out to evaluate the anti-inflammatory property of ethnomedicinal plants Viscum articulatum and Acorus
Methods: Human red blood cells membrane stabilization method was applied to assess the anti-inflammatory property of both the plants.
Results: It was observed that both plant extracts have the anti-inflammatory potential comparable with that of the drug indomethacin. However, A.
calamus was found to provide slightly more inhibition of hemolysis (76.8%) than that of standard drug (indomethacin) (72.8%), whereas V. articulatum
provided slightly lesser inhibition (68%) in comparison to the drug. There was the tendency of increase protection along with the increase in the
concentration of the extract, and maximum protection was achieved at the maximum concentration of 5000 Âµg/ml.
Conclusion: The study proves the anti-inflammatory efficacy of both the plants and they hold a good prospect for drug development against
Keywords: Anti-inflammatory, Viscum articulatum, Acorus calamus, Sikkim.
Srivastava TN, Kapaki BK. Resource survey of plants of potential
economic value of Sikkim Himalaya. Bull Medico Ethno Bot Res
Panda AK, Misra S. Health traditions of Sikkim Himalaya. J Ayurveda
Integr Med 2010;1(3):183-9.
Das B. Encyclopedia of Tibetan Medicine. Vol. 1-3. Delhi: Sri Satguru
Publication; 1994. p. 103-10.
Singh G. Recent considerations in nonsteroidal anti-inflammatory drug
gastropathy. Am J Med 1998;105(1B):31S-8.
Griffin MR, Yared A, Ray WA. Nonsteroidal anti-inflammatory
drugs and acute renal failure in elderly persons. Am J Epidemiol
Hossain MM, Ahamed SK, Dewan SM, Hassan MM, Istiaq A,
Islam MS, et al. In vivo antipyretic, antiemetic, in vitro membrane
stabilization, antimicrobial, and cytotoxic activities of different extracts
from Spilanthes paniculata leaves. Biol Res 2014;47(1):45.
Joseph J, Bindhu AR, Aleykutty NA. In vitro and in vivo
Anti-inflammatory activity of Clerodendrum paniculatum Linn.
Leaves. Indian J Pharm Sci 2013;75(3):376-9.
Saleem TK, Azeem AK, Dilip C, Sankar C, Prasanth NV, Duraisami R.
Anti-inflammatory activity of the leaf extacts of Gendarussa vulgaris
Nees. Asian Pac J Trop Biomed 2011;1(2):147-9.
Chippada SC, Volluri SS, Bammidi SR, Vangalapati M. In vitro
anti-inflammatory activity of methanolic extract of Centella asiatica by
HRBC membrane stabilisation. Rasayan J Chem 2011;4(2):457-60.
Shailesh G, Seema K, Dwivedi S. In-vitro anti-inflammatory activity
of Sarcostemma acidum Wight. and Arn. Indian herb by Human red
blood cell membrane stabilization method. Int J Pharm Teach Pract
Panda AK. Medicinal plants use and primary health care in Sikkim. Int
J Ayurvedic Herb Med 2012;2(2):253-9.
Joshi P. Ethnobotany of the Primitive Tribes in Rajasthan. Jaipur, India:
Printwell; 1995. p. 89.
Chou CT. The anti-inflammatory effect of Tripterygium wilfordi Hook
on adjuvant induced paw edema in rats and inflammatory mediatorâ€™s
release. Phytother Res 1997;17:152-4.
Sadique JA, Al-Rqobah WA, Bugharith ME, El-Gin-dy AR. The
bioactivity of certain medicinal plants on the stabilization of RBC
membrane system. Fitoterapia 1989;6:525-32.
Vijayasarathy V, Sharma LK, Prakash A. Indigenous drug treatment for
hemorrhoids. Probe 1981;120:285-7.
Kim H, Han T, Lee SG. Anti-inflammatory activity of a water extract of
Acorus calamus Leaves on keratinocyte HaCa T cells. J Ethnopharmacol
Shi GB, Wang B, Wu Q, Wang TC, Wang CL, Sun XH, et al.
Evaluation of the wound-healing activity and anti-inflammatory
activity of aqueous extracts from Acorus calamus L. Pak J Pharm Sci
Di Rosa M, Giroud JP, Willoughby DA. Studies on the mediators of
the acute inflammatory response induced in rats in different sites by
carrageenan and turpentine. J Pathol 1971;104(1):15-29.
Saha C, Hegde P, Friboulet A, Bayry J, Kaveri SV. Viscum
album - mediated COX-2 inhibition implicates destabilization of
COX-2 mRNA. PLoS One 2015;10(2):e0114965.
Othman RA, Moghadasian MH. Beyond cholesterol-lowering effects of
plant sterols: Clinical and experimental evidence of anti-inflammatory
properties. Int Life Sci Inst 2011;69(7):371-82.
Kam PC, Liew S. Traditional Chinese herbal medicine and anaesthesia.
Leu YL, Kuo SM, Hwang TL, Chiu ST. The inhibition of superoxide
anion generation by neutrophils from Viscum articulactum. Chem
Pharm Bull (Tokyo) 2004;52(7):858-60.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.