STUDY OF THE POSSIBLE ANTIHYPERTENSIVE AND HYPOLIPIDEMIC EFFECTS OF AN HERBAL MIXTURE ON L-NAME-INDUCED HYPERTENSIVE RATS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i5.12175Abstract
Objective: Hypertension is a chronic medical condition. Diet can improve blood pressure control and decrease the risk of health complication.
Methods: In this study, four plants: Roselle, Marjoram, Chamomile, and Doum were extracted by water. Equal portions of them were mixed. Lethal
dose 50% of the mixture was assayed; the dose which did not cause any mortality was 266.94 mg/100 g body weight. Animals were classified into five
groups: Negative control group, positive control group where hypertension was induced by L-name, two groups treated with two doses of the mixture,
and a group treated with prazosin as a standard treatment. Treatment of hypertensive rats continued for 4 successive weeks.
Results: Treatment with the mixture showed a significant reduction in blood pressure of hypertensive rats, as well as serum cholesterol, low-density
lipoprotein-cholesterol, and urea levels when compared to positive control group.
Conclusion: The results obtained suggest that the aqueous extract is efficient as an antihypertensive and hypolipidemic agent.
Keywords: Rats, Aqueous extract, Hypertension, Hyperlipidemia, L-name.
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References
World Health Organization. Regional Office for Southeast Asia. Hypertension Fact Sheet. Available from: http://www.searo.who.int/linkfiles/non_communicable_diseases_hypertension-fs.pdf. [Last accessed on 2012 Apr].
Ames RP. Hyperlipidemia in hypertension: Causes and prevention. Am Heart J 1991;122(4):1219-24.
Heran BS, Galm BP, Wright JM. Blood pressure lowering efficacy of alpha blockers for primary hypertension. Cochrane Database Syst Rev 2009;7(4):CD004643.
Finnerty FA Jr, Davidov M, Mroczek WJ, Gavrilovich L. Influence of extracellular fluid volume on response to antihypertensive drugs. Circ Res 1970;27 1 Suppl 1:71-82.
Chait A, Brunzell JD. Acquired hyperlipidemia (secondary dyslipoproteinemias). Endocrinol Metab Clin North Am 1990;19(2):259-78.
Wahabi HA, Alansary LA, Al-Sabban AH, Glasziuo P. The effectiveness of Hibiscus sabdariffa in the treatment of hypertension: A systematic review. Phytomedicine 2010;17(2):83-6.
Kim IH, Kim C, Seong K, Hur MH, Lim HM, Lee MS. Essential oil inhalation on blood pressure and salivary cortisol levels in prehypertensive and hypertensive subjects. Evid Based Complement Alternat Med 2012;2012:984203.
Srivastava JK, Shankar E, Gupta S. Chamomile: A herbal medicine of the past with bright future. Mol Med Rep 2010;3(6):895-901.
El-Gendy AA, El-Mileegy A, Ghyaty ED, Malek HA, El-Hamid M, Amany A. The beneficial dietary hypotensive and hypolipidaemic effects of Hyphaene thebaica (Doum). Int J Altern Med 2008;7(1):1.
Guenther E. The Essential Oils. Vol. 1. New York: D. Van-Nostrands Co.; 1953. p. 526-48.
Lewis H, Smith C. Sugar alcohols in fungi and green plants. Methods of detection and estimation. New Phytol 1967;66:185-204.
Shellard E. Practical Plant Chemistry. London: London Ritman Medical Publishing Co., Ltd.; 1957. p. 34-80.
Harborne JB. Phytochemicals Methods. London: Chapman and Hall Ltd.; 1973. p. 49-188.
Trease GE, Evans WC. Pharmacognosy. 11th ed. London: Bailliere Tindall: 1989. p. 45-50.
Shinoda J. Color reactions of flavone and flavonol derivatives. J Pharm Soc 1928;48:214-20.
Hanson JR. In: Newman AA, editor. Chemistry of Terpenes and Terpenoids. New York: Academic Press; 1972. p. 155-206.
Sofowara A. Medicinal Plants and Traditional Medicine in Africa. 3rd ed. Nigeria: Spectrum Books Ltd.; 1993. p. 150-153.
Karber G. Contribution to the collective treatment of pharmacological serial experiments. Arch Exp Pathol Pharm 1931;162:480-2.
Khayyal MT, el-Ghazaly MA, Abdallah DM, Nassar NN, Okpanyi SN, Kreuter MH. Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats. Arzneimittelforschung 2002;52(11):797-802.
Paget GE, Barnes JM. Evaluation of drug activities. In: Lawrence DR, Bacharach AL, editors. Pharmacometics. Vol. 1. New York: Academic Press; 1964. p. 161.
Irvine RJ, White J, Chan R. The influence of restraint on blood pressure in the rat. J Pharmacol Toxicol Methods 1997;38:157-62.
Sorg DA, Buckner B. A simple method of obtaining venous blood from small laboratory animals. Proc Soc Exp Biol Med 1964;115:1131-2.
Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem 1973;19(12):1350-6.
Fossati P, Prencipe L. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 1982;28(10):2077-80.
Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res 1970;11(6):583-95.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499-502.
Fawcett JK, Scott JE. A rapid and precise method for the determination of urea. J Clin Pathol 1960;13:156-9.
Schirmeister J. Creatinine forms a colored complex with picrate in an alkaline medium. Dtsch Med Wschr 1964;89:1018-640.
Liener IE. Toxic Constituents of Plant Food Stuffs. 2nd ed. New York: Academic Press; 1980. p. 286.
Sung JH, Jo YS, Kim SJ, Ryu JS, Kim MC, Ko HJ, et al. Effect of lutein on L-NAME-induced hypertensive rats. Korean J Physiol Pharmacol 2013;17(4):339-45.
Bachmann S, Mundel P. Nitric oxide in the kidney: Synthesis, localization, and function. Am J Kidney Dis 1994;24(1):112-29.
Bilanda DC, Dimo T, Dzeufiet Djomeni PD, Bella NM, Aboubakar OB, Nguelefack TB, et al. Antihypertensive and antioxidant effects of Allanblackia floribunda Oliv. (Clusiaceae) aqueous extract in alcohol- and sucrose-induced hypertensive rats. J Ethnopharmacol 2010;128(3):634-40.
Koffi NF, Marie-Solange T, Emma AA, Noel ZG. Ethno botanical study of plants used to treat arterial hypertension, in traditional medicine, by abbey and Krobou Population of Agboville. Eur J Sci Resvol 2009;35:85-98.
El-Gendy AA, El-Mileegy A, Ghyaty ED, Malek HA, El-Hamid M, Amany A. The beneficial dietary hypotensive and hypolipidaemic effects of Hyphaene thebaica (Doum). Int J Altern Med 2009;7:1.
Minato K, Miyake Y, Fukumoto S, Yamamoto K, Kato Y, Shimomura Y, et al. Lemon flavonoid, eriocitrin, suppresses exercise-induced oxidative damage in rat liver. Life Sci 2003;72(14):1609-16.
Daniel RS, Devi KS, Augusti KT, Sudhakaran Nair CR. Mechanism of action of antiatherogenic and related effects of Ficus bengalensis Linn. flavonoids in experimental animals. Indian J Exp Biol 2003;41(4):296-303.
Han LK, Zheng YN, Xu BJ, Okuda H, Kimura Y. Saponins from platycodi radix ameliorate high fat diet-induced obesity in mice. J Nutr 2002;132(8):2241-5.
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