BIOACTIVE POTENTIAL OF STREPTOMYCES VARIABILIS - DV-35 ISOLATED FROM THOTTADA MARINE SEDIMENTS, KANNUR, KERALA
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s3.14643Abstract
ABSTRACT
Objective: This study was aimed to evaluate the bioactive potential (antimicrobial and antioxidant) of marine actinobacteria isolated from Thottada
marine sediment, Kannur, Kerala.
Methods: The study involved isolation of marine actinobacteria from Thottada marine sediments, followed by antimicrobial and antioxidant activity
of the crude extract. Finally, identification of potential isolates.
Results: In this study, a total of 6 actinobacterial colonies were isolated, out of which only one isolate DV-35 showed maximum anti-microbial and
anti-oxidant activity. The isolate DV-35 showed maximum inhibitory activity against Gram-negative bacterial pathogens Pseudomonas aeruginosa
(18 mm) followed by Salmonella typhi (17 mm) and Klebsiella sp. (13 mm) at a dose of 100 μg/ml. In antifungal activity, extract showed maximum
activity against Aspergillus niger (19 mm) followed Aspergillus flavus (17 mm) at a dose of 100 μg/ml. Simultaneously the extract was evaluated for its
antioxidant activity. The potential isolate DV-35 also showed moderately higher antioxidant activity in various screening assay, viz., 2,2-diphenyl-1-
picrylhydrazyl scavenging assay (80.12%), metal chelating assay and reducing power assay (76%) at a concentration of 100 μg/ml. Potential isolate
DV-35 was identified as Streptomyces variabilis DV-35 using molecular 16S rRNA sequencing.
Conclusion: The results obtained suggest that the extract is having bioactive metabolites that can be considered as a potential source for antimicrobial
and anti-oxidant drug development.
Keywords: Marine actinomycetes, Anti-microbial activity, Anti-oxidant activity, Pseudomonas aeruginosa, Aspergillus niger, Streptomyces variabilis.
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