EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i4.16930Abstract
Objective: The intent  of this report  was to investigate the effect of aqueous root extract of Clerodendrum serratum L. for antioxidant activity using divergent models viz. DPPH scavenging assay, Superoxide scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay.
Materials and Methods: The root of C. serratum was extracted using water. The yield of aqueous extract was 10%w/w. The outcome was examined statistically by the regression method.
Results and discussions: The IC50 values are 85.43 µg/ml and 107.59 µg/ml for DPPH radical scavenging and Superoxide scavenging assay respectively whereas  FRAP showed significant reducing power activity with increased concentration of sample. The pilot study showed, a significant correlation existed between concentrations of the extract and percentage engrossment of free radicals.
Conclusion: The antioxidant property may be corresponding to the polyphenols and flavonoids adjacent in the extract. These results clearly revealed that C. serratum might be effective against diseases analogous with free radical mediated.
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Keywords Clerodendrum serratum, DPPH, Superoxide, FRAP, Rutin, Antioxidant
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References
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Fig. 3: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of rutin
Fig. 4: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of aqueous root extract
Fig. 5: Ferric reducing antioxidant power assay showing antioxidant capacity based on the ability to reduce ferric ions of sample with increase in absorbance of sample and standard
Table 2: Superoxide scavenging assay
S.No
Concentration
(μg/ml)
Inhibition (%)
IC50 (μg/ml)
Rutin
Sample
Rutin
Sample
95±0.026
61±0.377
18
59
30±0.226
05±0.038
77±0.234
06±0.029
42±0.261
28±0.290
07±0.062
73±0.186
IC50: Inhibitory concentration 50%
Table 3: FRAP assay
S.No
Concentration
(μg/ml)
Absorbance
Rutin
Sample
134±0.007
094±0.005
162±0.023
127±0.009
189±0.006
149±0.008
216±0.005
181±0.011
244±0.020
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