GREEN TEA EXTRACT IN AN EYELASH GROWTH ENHANCER GEL FORMULATION: STABILITY TEST, EYE IRRITATION TEST, AND HUMAN EYELASH GROWTH ACTIVITY
Keywords:Green tea extract, Eyelash gel, Stability test, Nil, Eyelash growth activity
Objective: To formulate a green tea extract (GTE), which is often used as a hair growth product, to produce an eyelash gel with good stability, effectiveness, and safety for growing eyelashes.
Methods: GTE was formulated into a gel. A stability test was performed at a high temperature (40Â±2Â°C), room temperature (25Â±2Â°C), low temperature (4Â±2Â°C), and a cycling temperature. An in vitro hen's egg test-chorioallantoic membrane assay was performed to evaluate potential eye irritation. An eyelash growth test was conducted by length measurement using an eyelash ruler before and after 2 mo of application in human volunteers.
Results: The GTE gel was stable in storage at high, room, and low temperatures and at cycling temperatures and did not cause eye irritation. Eyelashes grew significantly more in the test group than in the placebo group after 2 mo of application (p<0.05).
Conclusion: GTE gel provides a new, safe, and effective option for growing natural eyelashes.
Hunt N, McHale S. The psychological impact of alopecia. BMJ 2005;331(7522):951-3.
Shaikh MY, Bodla AA. Hypertrichosis of the eyelashes from prostaglandin analog use: A blessing or a bother to the patient? J Ocul Pharmacol Ther 2006;22(1):76-7.
Cohen JL. Enhancing the growth of natural eyelashes: The mechanism of bimatoprost-induced eyelash growth. Dermatol Surg 2010;36(9):1361-71.
Draelos ZD. Cosmetic dermatology products and procedures 2nd ed. United Kingdom: Wiley Blackwell; 2016.
Kumar S, Singh N, Archana S. Green tea polyphenols: Versatile cosmetic ingredient. Int J Adv Res Pharm Bio Sci 2012;1:348-62.
Asyarie S, Immaculata MI, Triwahyuni E. Hair growth evaluation from combination of green tea extract and celery in a gel formulation. Acta Pharm Indonesia 2007;32:12-7.
Kwon OS, Han JH, Yoo HG, Chung JH, Cho KH, Eun HC, et al. Human hair growth enhancement in vitro by green tea epigallocatechin-3-gallate (EGCG). Phytomedicine 2007;14(7-8):551-5.
Eskes C. Eye Irritation. Available from: http://www.ec.europa.eu/consumers/sectors/cosmetics/files/doc/antest/(5)_chapter_3/3_eye_irritation_en.pdf. [Last accessed on 2016 Sep 13].
Vinardell MP, Mitjans M. Alternative methods for eye and skin irritation tests: An overview. J Pharm Sci 2008;97(1):46-59.
Cosmetic Europe. EU Ban on Animal Testing. Available from: https://www.cosmeticseurope.eu/safety-and-science-cosmetics-europe/alternative-methods/eu-ban-on-animal-testing.html. [Last accessed on 2016 May 14].
PETA. Cosmetics and Animal Testing. Available from: http://www.peta.org.uk/issues/animals-not-experiment-on/cosmetics/. [Last accessed on 2016 May 14].
Kanter J. E.U. Bans Cosmetics With Animal-tested Ingredients. 11th March, 2013. Available from: http://www.nytimes.com/2013/03/11/business/global/eu-to-ban-cosmetics-with-animal-tested-ingredients.html. [Last accessed on 2016 May 14].
Luepke NP. Henâ€™s egg chorioallantoic membrane test for irritation potential. Food Chem Toxicol 1985;23(2):287-91.
Cazedey EC, Carvalho FC, Fiorentino FA, GremiÃ£o MP, Salgado HR. CorrositexÂ®, BCOP and HET-CAM as alternative methods to animal experimentation. Braz J Pharm Sci 2009;45:759-66.
Gilleron L, Coecke S, Sysmans M, Hansen E, Van Oproy S, Marzin D, et al. Evaluation of a modified HET-CAM assay as a screening test for eye irritancy. Toxicol In Vitro 1996;10(4):431-46.
Djajadisastra J. Cosmetic Stability. Seminar Setengah Hari HIKI. Department of Pharmacy, Faculty of Mathematics and Sciences, University of Indonesia; 2003.
Yongho, H. Eyelash measuring device. Patent US 9226537 B1. 5 January; 2016.
Rusak G, Komes D, Likic S, Horzic D, Kovac M. Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used. Food Chem 2008;110:852-8.
Hu CJ, Gao Y, Liu Y, Zheng XQ, Ye JH, Liang YR, et al. Studies on the mechanism of efficient extraction of tea components by aqueous ethanol. Food Chem 2016;194:312-8.
Rowe RC, Sheskey PJ, Quin ME. Handbook of Pharmaceutical Excipient. USA: Pharmaceutical Press and American Pharmacist Association; 2009.
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