CORRELATION OF (PRO)RENIN RECEPTOR AND VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION LEVEL IN THIRD TRIMESTER OF PREECLAMPSIA PLACENTAS

Nelly Marissa; Sri Widia A Jusman; Yuditiya Purwosunu; Ani Retno Prijanti

doi:10.22159/ajpcr.2018.v11i12.23173

Authors

Nelly Marissa Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Sri Widia A Jusman Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Yuditiya Purwosunu Department of Biochemistry and Molecular Biology, Centre of Hypoxia and Oxidative Stress Studies, FKUI, Jakarta, Indonesia 10430.
Ani Retno Prijanti Department of Biochemistry and Molecular Biology, Centre of Hypoxia and Oxidative Stress Studies, FKUI, Jakarta, Indonesia 10430.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i12.23173

Keywords:

Preeclampsia, Placenta, Vascular endothelial growth factor, (Pro) renin receptor(P)RR

Abstract

Objective: This cross-sectional study was designed to correlate the expression of mRNA and protein of (pro)renin receptor ([P]RR) and vascular endothelial growth factor (VEGF) gene in preeclampsia placentas.

Methods: We used 34 placenta tissues of normal pregnancy and 34 preeclampsia placenta tissues. Real-time polymerase chain reaction was used to measure relative expression of VEGF and (P)RR mRNAs, and protein concentration was measured using Sandwich ELISA technique.

Results: VEGF mRNA relative expression of preeclampsia placenta was 2.83 times higher than the normal placenta (p=0.02). (P)RR mRNA relative expression on preeclampsia placenta was 1.7 times higher than the normal one (p=0.039). Expression of protein (P)RR and VEGF was lower on preeclampsia shown by p<0.05, R=0.441. From this research, it was considered that low protein of (P)RR and VEGF contributes to preeclampsia.

Conclusion: There was a correlation between (P)RR and VEGF. It considers that there is any involvement of (P)RR on angiogenesis through VEGF expression in preeclampsia placenta.

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Author Biography

Nelly Marissa, Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia

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