IN VITRO CYTOTOXIC ANTICANCER POTENTIAL OF BIOACTIVE FRACTION ISOLATED FROM INDONESIAN TIDAL SPONGE CALTHROPELLA SP.
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i1.23655Keywords:
Calthropella sp, 3-(4, 5-Dimethylthiazol-2-y1)-2, 5-diphenyltetrazolium bromide assay, MCF-7, H-460, HepG-2Abstract
Objective: This study was taken to examine the cytotoxicity of the bioactive fraction isolated from marine sponge Calthropella sp. as a preliminary anticancer assay and identify its bioactive compounds.
Methods: The cytotoxic activity was assessed by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide assay against three human cancer cell lines, namely human breast (MCF-7), human lung (H-460), and human liver (HepG-2). The bioactive compounds were identified using a high-resolution liquid chromatography–mass spectroscopy (LC–MS).
Results: The active fraction 7 showed moderate to strong cytotoxic activity on all cell lines tested and promising a strong potent cytotoxicity against MCF-7 cell lines with IC50 value as low as 1.925 μg/mL comparable to control, cisplatin (IC50 0.977 μg/mL). In regard to the promising bioactive compounds, the high-resolution LC–MS predicted the existing of several known compounds such as bengamide Q, clavepictine A, 4'-N-methyl-5'- hydroxystaurosporine, carteriofenone A, and one strong possibility of a new compound.
Conclusion: This study has revealed that the isolated bioactive fraction of Indonesian tidal sponge, Calthropella sp., possesses potential anticancer properties with a promising significant cytotoxicity on MCF-7 cell lines (IC50 1.925 μg/mL).
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References
World Health Organization. Global Status Report on Noncommunicable Diseases. Switzerland: WHO Press; 2011. p. 176.
Indonesian Ministry of Health. Riset Kesehatan Dasar 2013 Badan Litbangkes Dan Data Penduduk Sasaran. Jakarta: Pusdatin Kementerian Kesehatan RI; 2014. p. 306.
Florea AM, Büsselberg D. Cisplatin as an anti-tumor drug: Cellular mechanisms of activity, drug resistance and induced side effects. Cancers (Basel) 2011;3:1351-71.
Blunt JW, Copp BR, Hu WP, Munro MH, Northcote PT, Prinsep MR, et al. Marine natural products. Nat Prod Rep 2009;26:170-244.
Simmons TL, Andrianasolo E, McPhail K, Flatt P, Gerwick WH. Marine natural products as anticancer drugs. Mol Cancer Ther 2005;4:333-42.
Molinski TF, Dalisay DS, Lievens SL, Saludes JP. Drug development from marine natural products. Nat Rev Drug Discov 2009;8:69-85.
Mehbub MF, Lei J, Franco C, Zhang W. Marine sponge derived natural products between 2001 and 2010: Trends and opportunities for discovery of bioactives. Mar Drugs 2014;12:4539-77.
Graham-Evans B, Tchounwou PB, Cohly HH. Cytotoxicity and proliferation studies with arsenic in established human cell lines: keratinocytes, melanocytes, dendritic cells, dermal fibroblasts, microvascular endothelial cells, monocytes and tcells. Int J Mol Sci 2003;4:13-21.
Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 1988;48:589-601.
Susilawati S, Sabirin M, Harno DP, Chairil A. Antioxidant activity of 2,6,4’-trihydroxy-4-methoxy benzophenone from ethyl acetate extract of leaves of mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.). Indo J Chem 2011;11:180-5.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Lit M. A Database of the Marine Natural Product Literature. Royal Society of Chemistry (UK). Available from: http://www.pubs.rsc.org/ marinlit/. [Last accessed on 2015 Nov ???].
White KN, Tenney K, Crews P. The bengamides: A Mini-review of natural sources, analogues, biological properties, biosynthetic origins, and future prospects. J Nat Prod 2017;80:740-55.
Hernández LM, Blanco JA, Baz JP, Puentes JL, Millán FR, Vázquez FE, et al. 4’-N-methyl-5’-hydroxystaurosporine and 5’-hydroxystaurosporine, new indolocarbazole alkaloids from a marine Micromonospora sp. Strain. J Antibiot (Tokyo) 2000;53:895-902.
Raub MF, Cardellina JH 2nd, Choudhary MI, Ni CZ, Clardy J, Alley MC. Clavepictines A and B: Cytotoxic quinolizidines from the tunicate Clavelina picta. J Am Chem Soc 1991;113:3178.
Habli Z, Toumieh G, Fatfat M, Rahal ON, Gali-Muhtasib H. Emerging cytotoxic alkaloids in the battle against cancer: Overview of molecular mechanisms. Molecules 2017;22. pii: E250.
Ardiansah B. Recent reports on pyrazole-based bioactive compounds as candidate for anticancer agents. Asian J Pharm Clin Res 2017;10:45-51.
Ismail MM, Soliman DH, Farrag AM, Sabour R. Synthesis, antitumor activity, pharmacophore modelling and QSAR studies of novel pyrazolesand pyrazolo [1,5-a] pyrimidines against breast adenocarcinoma MCF-7 cell line. Int J Pharm Pharm Sci 2016;8:434-42.
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