SURVEILLANCE OF BACTERIA METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA IN PATIENTS ADMITTED TO ORTHOPEDIC DEPARTMENT IN A TERTIARY REFERRAL HOSPITAL

Authors

  • Pulin Bihari Das Department of Orthopedics, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan University, K-8, Kalinga Nagar, Bhubaneswar – 751 003, Odisha, India.
  • Monali Priyadarshini Mishra Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan University, K-8, Kalinga Nagar, Bhubaneswar – 751 003, Odisha, India.
  • Siba Narayan Rath Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan University, K-8, Kalinga Nagar, Bhubaneswar – 751 003, Odisha, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i6.24136

Keywords:

Surgical site infections, Hospital-acquired infection, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa

Abstract

Objective: Methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged independently in diverse geographic zones and MRSA and Pseudomonas aeruginosa cause surgical site infections. Nosocomial surveillance in orthopedic surgery wards of the hospital for 16 months is presented.

Methods: A total of 621 wound swabs were cultured on blood and MacConkey agar plates for bacteria and Sabouraud dextrose agar for fungi.

Results: From 468 bacterial colonies, 98 MRSA and 74 P. aeruginosa strains and 41 fungal strains were isolated, and fungal strains were 13 strains of Aspergillus niger, and 28 strains of Candida albicans. P. aeruginosa and S. aureus strains were susceptible to antibiotics tobramycin, ciprofloxacin, piperacillin, vancomycin, levofloxacin, and amoxyclav. Similarly, A. niger and C. albicans were susceptible to antifungals, amphotericin B (AMB), liposomal AMB, itraconazole, voriconazole, posaconazole, and caspofungin.

Conclusion: Isolated MRSA strains were resistant to presently used common antibiotics, which attribute to the leading causatives of post-operative infection in orthopedic wounds, specifically.

Downloads

Download data is not yet available.

References

WHO. Prevention of Hospital Acquired Infections: A Practical Guide. Malta: Department of Communicable Disease, Surveillance and Response; 2002. Available from: http://www.who.int/csr/resources/ publications/whocdscsreph200212.pdf. [Last accessed on 2010 Jul 20].

Endalafer N, Gebre-Selassie S, Kotisso B. Nosocomial bacterial infections in a tertiary hospital in Ethiopia. J Infect Prev 2011;12:38-43.

Napolitano MN. Perspectives in surgical infections: What does the Future hold? Surg Infect 2010; 11:111-23.

Datta R, Huang SS. Risk of infection and death due to methicillin-resistant Staphylococcus aureus in long-term carriers. Clin Infect Dis 2008;47:176-81.

Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in US hospitals: Analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 2004;39:309-17.

Nguyen D, MacLeod WB, Cam PD, Cong QT. Incidence and predictors of surgical-site infections in vietnam. Infect Control Hosp Epidemiol 2001;22:485-93.

Whitehouse JD, Friedman ND, Kirkland KB, Richardson WJ, Sexton DJ. The impact of surgical-site infections following orthopedic surgery at a community hospital and a university hospital: Adverse quality of life, excess length of stay, and extra cost. Infect Control Hosp Epidemiol 2002;23:183-9.

Pittet D. Compliance with hand disinfection and its impact on hospital-acquired infections. J Hosp Infect 2001;48 Suppl A: S40-6.

Tammelin A, Ljungqvist B, Reinm¨uller B. Comparison of three distinct surgical clothing systems for protection from air-borne bacteria: a prospective observational study. Patient Saf Surg 2012;15:23.

Dubey D, Rath S, Sahu MC, Patnaik L, Debata NK, Padhy RN. Surveillance of infection status of drug resistant Staphylococcus aureus in an Indian teaching hospital. Asian Pacif J Trop Dis 2013;3:133-42.

Rath S, Padhy RN. Surveillance of acute community acquired urinary tract bacterial infections. J Acute Dis 2015;3:186-95.

Mishra MP, Debata NK, Padhy RN. Surveillance of multidrug resistant uropathogenic bacteria in hospitalized patients in Indian. Asian Pac J Trop Biomed 2013;3:315-24.

Rath S, Panda M, Sahu MC, Padhy RN. Bayesian analysis of two diagnostic methods for paediatric ringworm infections in a teaching hospital. J Mycol Med 2015;25:191-9.

StarÄević S, Munitlak S, Mijović B, Mikić D, Suljagić V. Surgical site infection surveillance in orthopedic patients in the military medical academy, belgrade. Vojnosanit Pregl 2015;72:499-504.

Lilani SP, Jangale N, Chowdhary A, Daver GB. Surgical site infection in clean and clean-contaminated cases. Indian J Med Microbiol 2005;23:249-52.

Patel SM, Patel MH, Patel SD, Soni ST, Kinariwala DM, Vegad MM. Surgical site infections: Incidence and risk factors in a tertiary care hospital, Western India. Nat J Community Med 2012;3:193-6.

Mahesh CB, Shivakumar S, Suresh BS, Chidanand SP, Vishwanath Y. A prospective study of surgical site infections in a teaching hospital. J Clin Diagn Res 2010;4:114-9.

Malik S, Gupta A, Singh KP, Agarwal J, Singh M. Antibiogram of aerobic bacterial isolates from post-operative wound infections at a tertiary care hospital in India. J Infect Dis Antimicrob Agents 2011;28:45-52.

Al-Mulhim FA, Baragbah MA, Sadat-Ali M, Alomran AS, Azam MQ. Prevalence of surgical site infection in orthopedic surgery: A 5-year analysis. Int Surg 2014;99:264-8.

Uçkay I, Pittet D, Vaudaux P, Sax H, Lew D, Waldvogel F, et al. Foreign body infections due to Staphylococcus epidermidis. Ann Med 2009;41:109-19.

Momaya AM, Hlavacek J, Etier B, Johannesmeyer D, Oladeji LO,Niemeier TE, et al. Risk factors for infection after operative fixation of tibial plateau fractures. Injury 2016;47:1501-5.

Techaoei S, Eakwaropas P, Khemjira J, Warachate K. Structure characterization and evaluation potential of antimicrobial. Extracts from Phellinus linteus against skin infectious pathogens, Staphylococcus epidermidis atcc12228 and Propionibacterium acnes dmst14916. Int J Pharm Sci 2017;9:70-81.

Penta J, Jannu K, Musthyala R. Antimicrobial studies of selected antibiotics and their combination with enzymes. Int J Pharm Sci 2010;2:43-4.

Published

07-06-2018

How to Cite

Das, P. B., M. P. Mishra, and S. N. Rath. “SURVEILLANCE OF BACTERIA METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA IN PATIENTS ADMITTED TO ORTHOPEDIC DEPARTMENT IN A TERTIARY REFERRAL HOSPITAL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 6, June 2018, pp. 381-5, doi:10.22159/ajpcr.2018.v11i6.24136.

Issue

Section

Original Article(s)