ANALYSIS REACTIVITY OF PUNICA GRANATUM POLYPHENOLS TO THE OSTEOCALCIN, BONE MORPHOGENETIC PROTEIN-2, AND COLLAGEN TYPE-1
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i12.29683Keywords:
Punica granatum, Reactivity, Osteocalcin, Bone matrix protein-2, Collagen Type-1Abstract
Objective: Punica granatum (PG) contains anthocyanins which are useful as antioxidants, anti-inflammatory, and prevent cancer, while also increasing bone cell proliferation and osteoblasts differentiation in bone remodeling.
Methods: The reactivity of osteocalcin protein markers with PG polyphenol fractionation hydrogels observed using enzyme-linked immunosorbent assay, the degree of reactivity was determined by optical density at 550 nm.
Results: PG butanol fraction has better reactivity compared to the total extract, ethyl, and hexane fraction. Based on the reactivity distribution, bone morphogenetic protein (BMP)-2 and collagen Type-1 had a dominant distribution compared to osteocalcin, but the theses proteins had a strong relation (r = 0.8) with probability (P < 0.05).
Conclusion: PG butanol fraction had better reactivity to osteocalcin, BMP-2, and collagen Type-1 compared with total extract, hexane, and ethyl fraction. The four PG polyphenol fractionations have dominant reactivity to BMP-2.
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