A NOVEL REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF ALOE-EMODIN AND PIPERINE IN AYURVEDIC FORMULATION

LEENA TANDEL; REVATI SONONE; VANDANA JAIN

doi:10.22159/ajpcr.2020.v13i2.36326

Authors

LEENA TANDEL Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
REVATI SONONE Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
VANDANA JAIN Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i2.36326

Keywords:

Aloe-emodin, Piperine, Ayurvedic formulation, Reverse-phase high-performance liquid chromatographic, Validation

Abstract

Objective: The objective of this study was to develop and validate a novel, simple, rapid, accurate, and precise reverse-phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of aloe-emodin and piperine in the ayurvedic formulation as per the International Conference on Harmonization guidelines.

Methods: Chromatographic separation was achieved using a Prontosil C18 (250 × 4.6 mm, 5 μ), with a mobile phase consisting of 0.05% orthophosphoric acid and acetonitrile in the ratio of 50:50, at a flow rate of 1 ml/min and column temperature maintained at 28°C and ultraviolet (UV) detection at 225 nm.

Results: The retention time of aloe-emodin and piperine was found to be 9.38±0.2 min and 13.45±0.2 min, respectively. The linearity of aloe-emodin and piperine was tested in the range of 1–20 μg/ml. The correlation coefficient for aloe-emodin and piperine was found to be 0.998 and 0.997, respectively. The recovery values (98–102%) indicate a satisfactory accuracy. The percentage relative standard deviation for precision was found to be <2% which indicates that the method is precise.

Conclusion: A precise method for the simultaneous quantification of aloe-emodin and piperine was developed using high-performance liquid chromatography. Hence, the developed method can be used for quantitative and quality control analysis of formulations containing these phytoconstituents.

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Author Biography

LEENA TANDEL, Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

Associate Professor at oriental college of pharmacy, recipient of ugc-jrf in engg and tech.....successfully completed 2 minor research project granted by mumbai university.......several publications in national and international journal of good impact factor...

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