DETERMINATION OF ALLICIN IN ALLIUM SATIVUM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND STUDY OF GENOTOXIC EFFECT ON HUMAN LEUKOCYTES

Authors

  • Nishu Sekar Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu
  • Rajiv Sundaramoorthy Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu
  • Sristi Majumdar Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu
  • Krishnakshi Bhuyan Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu
  • Jyotirekha Das Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu
  • V G Abilash Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu

Abstract

Objectives: Allicin is an organosulfur compound featuring thiosulfinate functional group. The compound is formed in garlic after tissue damage, by
the action of enzyme alliinase on alliin. This study involves extraction of allicin from garlic using high-performance liquid chromatography (HPLC) and
study of it is the genotoxic effect on human leukocytes.
Methods: A simple and rapid reverse phase HPLC was used for the extraction of allicin. Quality allicin, in conjugation with chymosin, was used to
study its genotoxic effect on leukocytes.
Results: Garlic oil and garlic extract showed similar retention time, and we measured the products using genotoxic effects in human leukocyte culture
and it shows statistically not significant.
Conclusion: This study suggested that to take a lower concentration of garlic extracts benefits for health and these findings useful for further research.
Keywords: Allium sativum, Leukocytes, Chromosome, Genotoxic, High-performance liquid chromatography.

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Author Biographies

Nishu Sekar, Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu

Division of Biomolecules and Genetics, School of Biosciences and Technology

V G Abilash, Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu

Assistant Professor Senior,Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu

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Published

01-11-2015

How to Cite

Sekar, N., R. Sundaramoorthy, S. Majumdar, K. Bhuyan, J. Das, and V. G. Abilash. “DETERMINATION OF ALLICIN IN ALLIUM SATIVUM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND STUDY OF GENOTOXIC EFFECT ON HUMAN LEUKOCYTES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 6, Nov. 2015, pp. 153-6, https://journals.innovareacademics.in/index.php/ajpcr/article/view/8022.

Issue

Vol 8 Issue 6 (November - December) 2015

Section

Original Article(s)

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