Int J App Pharm, Vol 10, Issue 4, 2018, 74-81Original Article



Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies (Affiliated to Pondicherry University), Lawspet, Puducherry 605008, India

Received: 03 Mar 2018, Revised and Accepted: 24 May 2018


Objective: Titanium dioxide nanoparticles (TiO2 NPs) are widely used in pharmaceutical, cosmeceutical, biomedical and industrial applications. The adverse effects of TiO2 NPs are also increasing alarmingly. The purpose of this study is to investigate the toxicity of TiO2 NPs on biochemical and histological changes in different regions of brain in adult male Wistar rats.

Methods: Two different doses of TiO2 NPs (50 mg/kg b. w and 100 mg/kg b. w) administered orally for 14 d along with one control group, each group consisting of six animals. Standard biochemical methods were adopted for the estimation of enzymes alkaline phosphatase, 5’ nucleotidase, ATPases and gamma-glutamyl transpeptidase. Trace elements calcium, sodium, potassium and magnesium as well as metals like iron, zinc and copper were also estimated.

Results: When compared with the control group, the enzymes ATPases, ALP, 5’-NT and GGT activities were significantly decreased in both the TiO2 NPs treated groups. Ca, Na, Fe, Cu and TiO2 contents were significantly increased in both the experimental groups, while the K, Mg and Zn contents decreased. However, the changes in the parameters studied were more in 100 mg treated group (p<0.001) when compared to the 50 mg treated group (p<0.05and p<0.01). Moreover, it is also evident that different regions responded differently due to TiO2 NPs exposure. The changes were maximum in the cerebral hemisphere (p<0.001) followed by medulla oblongata (p<0.001) and cerebrum (p<0.05).

Conclusion: The results clearly imply that TiO2 NPs could impair the electrochemical gradient, ionic homeostasis and membrane stability in different regions of the rat brain.

Keywords: Nanoparticles, Titanium, Brain, Membrane enzymes, Trace metals


TiO2 NPs is one of the most widely applied engineered nanoparticles in the nanotechnology field, where it is consequently used in paints, paper, plastics, ceramics, toothpaste, food colorants, drug capsule, catalysts and wastewater treatment [1, 2]. The yearly production of TiO2 NPs in 2002 was approximately 3,000 tons per annum, and is estimated to increase to 2.5 million metric tons annually by 2025 [3]. It is reported that in sunscreen and cosmetic products approximately 50% of TiO2 NPs are used [4]. It is evident from previous studies that less than 100 nm NPs have a high level of photocatalytic activity [5]. The unique characteristics of TiO2 particle is that it can enter the human body quickly and then imposes potential health risks on human [6, 7]. The adverse toxic effects of TiO2 NPs have been studied in several animal models like rats, mice, rabbits and human following exposure by multiple routes of administration, including whole-body exposure, dermal exposure, gastric lavage, and inhalation [1, 2, 8, 9]. Few studies reported that inhaled or injected nanoparticles enter the systemic circulation and migrate to various parts to exert adverse effects [10, 11].

The effects of TiO2 NPs on hepatic and renal dysfunctions were demonstrated by several studies through the detection of different functional enzymes such as alanine aminotransferase (ALT) and aspartic aminotransferase (AST) [1, 12-15]. Our previous study in this laboratory also observed that TiO2 NPs significantly alter these enzymes affecting liver and kidney of rats [16]. Nanoparticles can cross the blood-brain barrier (BBB) and enter in the central nervous system (CNS) of the exposed animals [11, 17]. Dermal exposure of TiO2 NPs could penetrate up to the brain via the skin as it was detected in the brain of mice [18]. TiO2 NPs could impair the short and long-term synaptic plasticity and also reactive oxygen species (ROS) mediated oxidative stress-induced damage and inflammatory response in rat brain [19]. TiO2 NPs were also shown to stimulate excess ROS generation in the brain microglial cells and cause neuron damages [20].

CNS is a main target for various environmental pollutants and xenobiotic chemicals, including heavy metals and metal oxides. Airborne metal dust or fumes is a foremost way of occupational exposure, causing acute and chronic disorders such as metal fume fever and chronic obstructive pulmonary disease (COPD) [21]. Direct disruption of neuronal cell membranes by nanoparticles would allow their entry into the brain [6, 21, 22]. Membrane-bound enzymes such as alkaline phosphatase (ALP), 5’ nucleotidase (5’-NT) and gamma-glutamyl transpeptidase (GGT) are predominantly concentrated in the vascular endothelium in the brain. Membrane ATPases are primary active transporters of cations that maintain a steep gradient and that form the basis for a range of essential cellular physiological processes. ATPase activity is an important toxicological tool to analyze the effect of xenobiotics.

Metal ions such as calcium (Ca), sodium (Na), potassium (K), iron (Fe), copper (Cu), zinc (Zn) and magnesium (Mg) are vital to life and participate in numerous metabolic processes in every living cell with considerable specificity and selectivity as components of enzymes and other molecules complexes. These metals have to be maintained at a fixed level to avoid any toxic effects. Metal homeostasis imbalance and neuronal loss are both present in neurodegenerative diseases [23]. To gain new insights into the mechanisms underlying the brain membrane damage mediated trace metal disturbances caused by TiO2 NPs, this study was aimed to investigate the penetration of TiO2 NPs and further biochemical changes in the brain by administering TiO2 NPs through intragastric route to Wistar rats, by assessing the changes in membrane-bound enzymes, and trace metals in different brain regions.


Chemicals and dose preparation

TiO2 NPs (CAS NO: 13463-67-7) used in the present study was purchased from Sigma-Aldrich Chemicals Co. (St, Louis, MO 63103, USA). The crystal profile as characterized by automatic X-ray diffractometer (XRD, PANlytical XPERT-PRO) equipped with 240 mmradius goniometer. The data were collected in the mode of continuous scanning with a power setting of 40kV, 30 mA, scanning speed of 10 °/sec using Cu Kα radiation (λ=0.154 nm). TiO2 nanoparticles were suspended in water and ultrasonicator for 5-20 min. The suspension was dipped on the cleaned silicon wafer and dried in an oven at 45 °C. The morphology and particle size of TiO2 nanoparticles were obtained with a high-resolution transmission electron microscopy (TEM, FEI Tecnai G2 F30S-Twin microscope).

A 0.9 % saline solution (NaCl) was used as a suspending agent. TiO2 NPs suspension was prepared using physiological saline solution. The powdered TiO2 NPs were dispersed in the fresh sterilized physiological saline solution, and the suspension was ultrasonicated for 15-20 min and mechanically vibrated for 2-3 min to get a homogenous suspension and to disperse completely as much as possible.

Animals and treatment

Healthy adult male albino rats of Wistar strain (Rattus norvegicus) weighing 250-260 g obtained from the central animal house, central siddha research institute, Chennai were used for the present investigation. The animals were fed ad libitum with standard pellet diet and had free access to drinking water. All experimental and protocols described in the present study was approved by the institutional animal ethics committee (IAEC Approval No. 138/PHARMA/SCRI, 2013) and are in accordance with guidelines as per “guide for the care and use of laboratory animals” published by NIH publication (NO 85-23 revised 1996) and with permission from committee for the purpose of control and supervision of experiments on animals (CPCSEA), Ministry of Social Justice and Empowerment, Government of India.

Animals were randomly divided into three groups.

Group I–Control: Rats were given a normal saline solution as vehicle orally by gavage, daily for 14 d.

Group II–TiO2 NPs treatment: Rats were treated with TiO2 NPs dissolved in normal saline solution at a dose of 50 mg/kg body weight daily for 14 d, orally by gavage.

Group III–TiO2 NPs treatment: Rats were treated with TiO2 NPs dissolved in normal saline solution at a dose of 100 mg/kg body weight daily for 14 d, orally by gavage.

At the end of the experiment, in order to maintain comparable conditions blood samples are usually collected at the morning after an overnight fasting. The fixed time schedule eliminates the possible effect of diurnal variations. 24 h after the experimental period the animals were sacrificed by cervical decapitation. Blood was collected separately for serological and haematological analyses. Brain was removed, cleared off the adhering tissues and weighed. Brain was dissected out; cerebrum, cerebellum and medulla oblongata were separated and processed immediately for biochemical and histological analysis. All the biochemical estimations were carried out by standard spectrophotometric techniques.

Membrane-bound enzymes assay

The brain samples were cooled in ice-cold, 50 mmol Tris–HCl buffer (pH 7.55) and homogenized. The homogenate was centrifuged for 10 min at 3000 rpm, the supernatant was used for the assay of ATPases, ALP, 5’-NT and GGT.

The ATPases activity estimated according to the method of Takeo and Sakanashi, [24]. The activities of total, Na+/K+, Mg2+ and Ca2+ATPases were determined using appropriate inhibitors of ATP; the inorganic phosphate liberated was estimated by the method of Fiske and Subbarow, [25]. Appropriate standard (sodium dihydrogen phosphate) was also run in each batch of the assay. The non-specific activity was subtracted from each activity detected. The enzyme activity was calculated from the standard graph. The enzyme activity is expressed as units/mg protein.

The activity of ALP was assayed by the method of Bessey et al. [26]. Each 0.5 ml reaction mixture contained 0.5 ml of glycine buffer and the mixture was placed in a water bath at 37 °C for 5 min. exactly after 30 min, the reaction was arrested by the addition of 10 ml of sodium hydroxide. The colour developed was read at 410 nm, in a spectrophotometer. 0.1 ml of concentrated HCL was added, mixed and the optical density was read at 400 nm. The ALP activity was calculated from the calibration curve obtained using para-nitrophenol standard. ALP activity is expressed as units/mg protein.

5’-NT was assayed following the method of Gerlach and Hiby, [27]. The activity of 5’-NT was measured by the selective complete inhibition of the enzymes by nickel ions. The assay is carried out in the presence and absence of nickel ions, in their presence the activity of nonspecific phosphatase was determined, while in their absence the sum of the activity of nonspecific phosphatase and 5’-NT was measured. The difference in the amount of phosphate ion liberated per unit in the two assays is a measure of 5’-NT activity expressed as units/mg protein.

The GGT activity was estimated by the colorimetric method of Orlowski and Meister, [28]. The enzyme catalyses the transfer of γ-glutamyl groups from γ-glutamyl donors to the amino acids, peptide or water. The sample contains 0.5 ml of the substrate, 1 ml Tris-HCl buffer and 2.2 ml glycylglycine. 0.2 ml of enzyme solution was added to the above mixture then incubated. After incubation for 30 min at 37˚C, the reaction was arrested by the addition of 1 ml of 10% acetic acid to the test and control tubes. The control tubes received substrate after incubation. Standard p-nitroaniline was also treated similarly. The amount of liberated p-nitroaniline in the supernatant was with and without the substrate. The substrate incubated in the absence of enzyme under the same condition was used as a reference blank. The enzyme activity was expressed as micromoles of p-nitroaniline formed/min/mg protein of tissue extract.

Metal content analysis

Approximately 0.2–0.3 g of tissues were weighed, digested and analyzed form metal contents. Briefly, prior to elemental analysis, these tissues were digested with ultrapure grade nitric acid overnight. After adding 0.5 ml hydrogen peroxide (H2O2), the mixed solutions were placed at 160 °C with high-pressure reaction containers in an oven-chamber until the samples were completely digested. Then, the solutions were incubated at 120 °C to remove the remaining nitric acid until the solutions were colourless and clear. Finally, the remaining solutions were diluted to 3 ml with 2% nitric acid. Inductively coupled plasma-mass spectrometry (ICP-MS) (Agilent 7500 CE, USA) was used to determine the titanium concentration in the samples. Indium 20 ng/ml was chosen as an internal standard element. The detection limit of titanium was 0.074 ng/ml. Data are expressed as nanograms per gram tissue.

Histological examination

For histology, Different brain regions were fixed for 48 h in 10% formalin-saline and dehydrated bypassing successfully through different mixtures of ethyl alcohol, cleaned in xylene and embedded in paraffin wax. Sections of the tissue (5–6 µm thick) were prepared using a rotary microtome, stained with haematoxylin and eosin (HandE) dye and then mounted in a neutral deparaffinized xylene medium for microscopic examination.

Statistical analysis

The data were subjected to statistical analysis using one-way analysis of variance (ANOVA) according to Zar, [29]. When the F test was found to be significant, the data were subjected to Student-Newman-Keuls (SNK) test to assess the significance of individual variations between the treatment groups.


Characterization of TiO2 nanoparticles

The microstructure, morphology, particle size and crystal profile of TiO2 nanoparticles were characterized by XRD and subsequent observation by TEM (fig. 1). The crystalline structure of TiO2 nanoparticles is clearly evident from the observation by TEM (fig. 1A). It is well evident from the XRD characterization the particle size of the TiO2 nanoparticles used in this study is having the size 33.95 nm (fig. 1B).

Fig. 1: Morphology and microstructure characterized by TEM (A) and X-ray diffraction pattern (B) of TiO2 nanoparticles

Membrane-bound enzymes

Fig. 2(A) shows the activity of Ca2+ dependent ATPase in cerebrum, cerebellum and medulla oblongata of the control and experimental rats. In 50 mg TiO2 NPs exposed rat group, a major inhibition of Ca2+dependent ATPase activity was observed in the cerebellum (p<0.001) followed by medulla oblongata (p<0.01) and cerebrum (p<0.05). In 100 mg TiO2 NPs treated group, the activity of Ca2+ dependent ATPase was significantly inhibited (p<0.001) in all the three regions. The reduction of this enzyme due to TiO2 NPs exposure is more and almost same (34%) in cerebellum and medulla oblongata when compared to cerebrum where the reduction was only 27%.

Fig. 2(B) shows the activity of Mg2+ dependent ATPase in control and experimental rats exposed to TiO2 NPs. The activity of Mg2+dependent ATPase was significantly decreased in the cerebrum (p<0.01) and cerebellum (p<0.05) of 50 mg TiO2 NPs treated groups whereas, no significant change was observed in medulla oblongata. In 100 mg treated group a noteworthy reduction (p<0.001) was observed in all the three regions. The reduction of this enzyme due to TiO2 NPs exposure is almost same in cerebellum and medulla oblongata (27%) when compared to cerebrum where the reduction was only 22%.

Fig. 2: Activities of Ca2+ ATPase (A), Mg2+ ATPase and Na+/K+ ATPase in the cerebrum, cerebellum and medulla oblongata of rats exposed to 50 and 100 mg/kg b. w of TiO2 NPs for 14 d. The results are expressed as mean±SEM (n = 6) per treatment and respective control groups. Levels of significance values are *p<0.05, **p<0.01 and ***p<0.001 compared with control group. *p<0.05 considered to be statistically significant

Fig. 2(C) shows the effect of TiO2 NPs on the activities of Na+/K+ dependent ATPase in control and experimental rat groups. In 50 mg exposed rats, the activity of Na+/K+ dependent ATPase was significantly reduced only in the cerebrum (p<0.05) and medulla oblongata (p<0.01), while there were no significant changes observed in the cerebellum. On other hand, in 100 mg administered group much more reduction of the activity of Na+/K+ dependent ATPase in the cerebellum (p<0.001) and medulla oblongata (p<0.001) followed by cerebrum (p<0.01) was noticed. The reduction of this enzyme almost same in cerebrum and cerebellum (37%) when compared to medulla oblongata where the decrement was only 27%.

Fig. 3(A) shows the activity of ALP in the brain of rats treated with TiO2 NPs. The activity of ALP is significantly reduced in the cerebrum (p<0.01) and cerebellum (p<0.001), whereas this enzyme activity was unaltered in the medulla oblongata of 50 mg TiO2 NPs treated group. The ALP activity was further reduced in 100 mg treated group in all the three regions (p<0.001).

The changes in the activity of 5’-NT of rat cerebrum, cerebellum and medulla oblongata is shown in fig. 3(B). In cerebrum and medulla oblongata regions, the activity of 5’-NT was not influenced significantly by TiO2 NPs in both the experimental groups. Interestingly, this enzyme activity was significantly decreased (p<0.01) in the cerebellum of 100 mg treated group.

The result presented in the fig. 3(C) indicates the activity of GGT in the control and experimental rats. TiO2 NPs did not alter the GGT activity in medulla oblongata exposed for 50 mg treated group, whereas significant reduction (p<0.01) was observed in cerebrum and cerebellum of the same group. In100 mg TiO2 NPs exposed group the activity was significantly reduced in the cerebrum (p<0.001), medulla oblongata (p<0.01) followed by cerebellum (p<0.05).

Fig. 3: Activities of ALP (D), 5’NT (E) and GGT (F) in the cerebrum, cerebellum and medulla oblongata of rats exposed to 50 and 100 mg/kg b. w of TiO2 NPs for 14 d. The results are expressed as mean±SEM (n = 6) per treatment and respective control groups. Levels of significance values are *p<0.05, **p<0.01 and ***p<0.001 compared with control group. *p<0.05 considered to be statistically significant

Metal content analysis

The contents of brain elements such as Ca, Mg, Na, K, Zn, Fe and Cu were determined. The contents of Ca, Mg, Na, K, Zn and Fe in rat brain regions are listed in table 1. It can be seen that exposure toTiO2 NPs has obviously changed the trace metal contents in the rat brain regions of cerebrum cerebellum and medulla oblongata. Increased contents of Ca, Na, Fe and Cu were found in different brain regions, while the contents of K, Mg and Zn were decreased in the same regions of brain from TiO2 NPs treated groups than those of the control.

Table 1: Effect of TiO2NPs on metal contents different regions of the adult male wistar rats

Metal contents

(µg/g tissue)

Experimental groups Cerebrum Cerebellum Medulla oblongata
Ca Control 175.40±7.57 156.80±7.08 138.70±6.94
50 mg/kg b. w. 228.80±10.08** 189.09±9.94* 149.90±6.37
100 mg/kg b. w. 238.20±11.78** 231.30±11.85*** 165.20±7.89*
Na Control 167.60±7.17 156.00±5.94 137.20±5.83
50 mg/kg b. w. 194.40±8.39* 182.70±7.15* 172.10±6.31
100 mg/kg b. w. 203.70±8.65** 183.30±7.72* 171.20±6.79**
K Control 2213.90±95.59 2247.50±101.50 1986.90±89.97
50 mg/kg b. w. 1910.00±78.78* 1908.00±76.53* 1668.30±69.08*
100 mg/kg b. w. 1903.70±78.57 1883.70±78.33* 1663.70±61.86*
Mg Control 624.70±24.77 596.00±23.43 398.00±14.44
50 mg/kg b. w. 608.40±22.93 499.40±21.07* 366.40±11.83
100 mg/kg b. w. 573.80±21.07 429.90±18.54*** 365.20±12.00
Fe Control 133.20±7.39 129.90±7.26 108.60±5.16
50 mg/kg b. w. 164.90±7.69* 169.70±7.43** 126.90±6.96
100 mg/kg b. w. 165.00±7.64* 171.60±7.82** 158.60±6.89***
Zn Control 94.40±4.53 86.20±4.29 64.60±3.66
50 mg/kg b. w. 80.60±3.78* 71.60±3.34* 48.10±2.72**
100 mg/kg b. w. 75.50±3.14** 70.40±2.75* 47.50±2.28**
Cu Control 12.10±0.56 16.30±0.86 9.00±0.49
50 mg/kg b. w. 16.10±0.74** 22.30±0.96*** 15.60±0.82***
100 mg/kg b. w. 19.60±0.97*** 25.00±1.04*** 15.00±0.76***

The results are expressed as mean±SEM (n = 6) per treatment and respective control groups. Levels of significance values are *p<0.05, **p<0.01 and ***p<0.001 compared with control group. *p<0.05 considered to be statistically significant.

Table 2: Titanium content in different regions of the rat brain after treatment

Ti (ng/g tissue) Cerebrum Cerebellum Medulla oblongata
Control Not detected Not detected Not detected
50 m/kg b. w. 173.20±8.16 165.90±8.19 149.90±4.40
100 mg/kgb. w. 225.60±12.66 221.80±11.99 160.90±6.68

The results are expressed as mean±SEM (n = 6) per treatment and respective control groups. In control rats, titanium was not detected.

Fig. 4: Histology of the brain tissues (cerebrum, cerebellum and medulla oblongata) in rat caused by oral administration with TiO2 NPs for consecutive 14 d. (A) Control g(100x) shows normal cerebral architecture; (B) 50 mg/kg b. w. TiO2 NPs group (100x): arrow indicates calcification deposition in neurocyte; suggesting too much of calcium depositing and accumulating in the cerebrum; (C) 100 mg/kg b. w. TiO2 NPs group (100x): arrow indicates ependyma proliferation; (D) control group (100x) shows normal cerebellum architecture and covering meninges (m); (E) 50 mg/kg b. w. TiO2 NPs group (100x): arrow indicates congestion and oedema in meninges covering the cerebellum; (F) 100 mg/kg b. w. TiO2 NPs group (100x): arrow indicates degeneration in purkinje cells; (G) control group (100x) shows normal medulla oblongata architecture; (H) 50 mg/kg b. w. TiO2 NPs group (100x): arrow indicates Axonal dystrophy, suggesting pathological changes of the neuron; (I) 100 mg/kg b. w. TiO2 NPs group (100x): arrow indicates Focal hemorrhage in the medulla oblongata. The section was stained with H and E and examined by light microscopy

Titanium content

The content of titanium in the rat brain regions are shown in table 2. With the increasing doses of TiO2 NPs, the titanium accumulation in cerebrum, cerebellum followed by medulla oblongata was elevated in both the groups, but not detected in control group.

Histology observations of the brain regions

Different regions of the brain histological pictures are illustrated in fig. 4. In the 50 and 100 mg/kg b. w. TiO2 NPs treated groups, the brain tissues had abnormal pathological changes compared with the control, suggesting calcification of neurocyte in the cerebrum, which is a result of too much Ca depositing and accumulating in the brain, the proliferation of ependyma and spongiocyte, respectively (fig. 4B, E and F). The generation of calcification and Ca depositions is confirmed by the assay of Ca content of brain regions.


Most toxicants interact with intracellular target molecules to cause changes in cellular metabolism and function, which eventually lead to cell membrane damage and cell lysis. Metal-induced plasma membrane damage occurs directly through interaction with membrane components such as ion-dependent ATPases and ion channels, and indirectly as a consequence of cytosolic damage [30].

The role of Mg2+ ATPase is to maintain the high intracellular Mg2+ level in brain, changes of which can alter the rate of protein synthesis and cell growth [31]. Mg2+ -ATPase is involved in ATP synthesis through oxidative phosphorylation in mitochondria [32]. The observed decrease in activity of Mg2+ ATPase in may hamper the ATP synthesis and thus can lead to mitochondrial disorganization [33].

Ca2+ ATPase, a Ca2+ binding protein and being crucial for maintenance of neuronal Ca2+ homeostasis [34], was significantly inhibited when exposed to TiO2 NPs. Calcium signalling is used by neurons to control a variety of functions, including cellular differentiation, synaptic maturation, neurotransmitter release, intracellular signalling, and cell death [35]. Ca2+ -ATPase is a Ca2+ pump that transfers Ca2+ from the cytosol to the lumen of the endoplasmic reticulum at the expense of ATP hydrolysis. Therefore, it regulates the intracellular Ca2+ and, in turn, numerous cellular processes, such as exocytosis, cell proliferation, gene transcription, muscle contraction, and cell survival [36].

Na+-K+ -ATPases play an important role in epithelial cell interaction and pathologies related with polarized epithelial cells and malignant tumors [37]. Na+/K+ -ATPase is a key enzyme implicated in neural excitability, metabolic energy production [37]. This enzyme has a fundamental role in the regulation of epithelial cell morphology, signal transduction, and cell signalling [38]. Na+/K+ -ATPase activity significantly decreased in the brain of TiO2 NPs treated mice and it was suggested that Na+/K+ -ATPase could not export intracellular redundant Na+ and/or import extracellular K+ timely. Decreased activity of Na+/K+ -ATPase activity in the brain of TiO2 NPs exposed rats, may reduce Na+ and K+ electrochemical gradient, disturbed ionic homeostasis and impair the physiological functions of neurons. In the present study, the altered activities of enzyme which are major biochemical component of cell membrane is suggestive of altered cellular proliferation and movement. It has been reported that metals interferes with the cell proliferation and cell migration [39].

ALP plays a crucial role in the metabolism of the CNS. ALP is associated with transmembrane transport mechanism, ion transport, maintenance of ionic strength and epithelial cell growth, differentiation and secretary activities [40]. ALP activity changes relatively under pathological conditions. In experimental brain wounds, high enzyme activity was reported in reorganizing capillaries at the borders of the lesion, in microglia cells, and in amorphous material within the brain wound [41]. It has been shown that the ALP is involved in the mediation of membrane transport and transphorylation [42]. The altered activity of ALP observed in the brain of TiO2 NPs treated rats suggests cell damage in the brain. The decrease in the activity of ALP in the present study by administration of TiO2 NPs could be attributed to either leakage of the enzyme into the extracellular fluid as a result of the disruption of the ordered lipid bilayer of the membrane or inhibition of the enzyme activity.

5'-NT is an established plasma membrane marker enzyme in many mammalian cells where it exists as an ectoenzyme [43]. The enzyme activity controls intracellular levels of nucleoside 5’ mono-phosphates, and it is a major contributor to the cascade that completely hydrolyze extracellular ATP to adenosine, and thus of major pharmacological interest. Reduction in the activity of 5'-NT was found in metal-treated group compared to control group [44]. The inhibition of 5′-NT due to TiO2 exposure may result in a decrease in extracellular adenosine production.

GGT is also considered as a marker enzyme of cell membrane. GGT couples γ-glutamyl moiety to a suitable amino acid acceptor for transport into the cell and makes it suitable for the intracellular synthesis of GSH. GGT also mediates the cleaving of the dipeptidyl cysteinyl glycine, which provides cells with cysteine, a rate-limiting factor for the synthesis of GSH [45].

Trace elements are essential for normal brain structure and functions. Tiny amounts of these elements help in the formation of neurotransmitters and involved in the antioxidant defence and intracellular redox regulation and modulation of neural cells [46]. In the present study, TiO2 NPs treatment increased Ca content in brain regions, which was consistent with the histological observation of the brain tissues. Brain injury involving in calcification and calcium deposition in neurocytes of TiO2 NPs exposed rats, which may indirectly or directly disturb the homeostasis of trace elements in brain as already suggested by Hu et al. [4]. Deficiency or excess of these metals resulted in central nervous system disorders [47] and pathological situation of the brain, such as neuronal injury, neurodegenerative diseases, and brain ischemia [48].

The present study shows the increased Ca and decreased Zn levels in various brain regions of rats exposed to TiO2 NPs. Thilsing-Hansen and Jørgensen, reported the antagonism between Ca and Zn in the brain following oral administration of ZnO NPs [49]. In the present study also TiO2 NPs shows the antagonism between Ca and Zn in different regions of the brain. The changes in the Ca and Zn content caused by TiO2 NPs can disturb the ion homeostasis and cause a series of physiological disorders in the CNS as it has been suggested earlier [34].

Fe as an important trace element is essential for neuron development and function. It is required for various physiological events including mitochondrial respiration, oxygen transport and DNA synthesis [50]. Furthermore, iron contributes to oxidative stress through Fenton reaction, leading to damages in DNA, proteins and membranes [51]. Fe imbalance is a precursor to the neurodegenerative processes leading to Alzheimer’s disease (AD) and quantification of brain Fe content can be an effective marker for early diagnosis of AD [51]. In the present study, TiO2 NPs treatment increased Fe content in different regions of rat brain. Excessive Fe would induce cell injury by reacting with H2O2 to produce hydroxyl radical (OH), superoxide anions (O2−), and ROS [53]. The Fe overload in the brain may lead to neuron damage [54]. Fe is necessary in many cellular functions, especially in the brain, where it participates in many neuronal processes. In excess Fe is toxic to cells. The brain continuously accumulates Fe, resulting in increased iron storage within the cell. This effect may lead to oxidative stress.

Zn is the second most abundant transition metal in the body [55], which is involved in the protection of BBB against oxidative stress of free radicals and essential for the synthesis of more than 300 coenzymes or metaloproteins that mediate biogenic amines synthesis and metabolism [56]. Zn level is essential to maintain homeostasis within the brain and prevent the development of neurological disorders [48]. A reduction in brain Zn content can impair the spatial memory in adult rats [57]. The observed reduction in the Zn content in different regions of the brain by TiO2 NPs may affect the function of Zn containing glutaminergic neurons and impairment of learning ability. This suggestion has already been put forth by Hu et al. in mice [4].

Cu is essential for normal CNS development and function, an essential cofactor in numerous enzymes, including cytochrome-c-oxidase (CcO), Cu/Zn superoxide dismutase (Cu, Zn-SOD) and ceruloplasmin (Cp) that play a central role for controlling the homeostasis of neurotransmitter and basic brain function [58]. The observed increased Cu level in the present study may disrupt the homeostasis in the brain and adverse effects in different regions of brain as it has also been shown in Fe2O3 NPs administered rats [59]. These findings support the hypothesis that neuronal cells from the brain regions of TiO2 NPs exposed rats undergo oxidative stress, which could ultimately lead to neurotoxicity as also suggested earlier [4, 60, 61]. The changes in electrochemical gradient and ionic homeostasis clearly indicate the damage to the brain of rats exposed to TiO2 NPs subsequently affecting the CNS.

Metals primarily affect the mammalian brain and it has the capacity to damage the developing as well as the mature brain. The exact chemical mechanism by which TiO2 NPs influences brain is not clear. From this study, it is evident that TiO2 NPs have a definite influence on membrane-bound enzymes and trace metals in the brain of male rats. The altered biochemical parameters strongly suggest the alteration in cell permeability, and possible cell injury and damage to CNS.


Our results clearly imply that orally ingested TiO2 NPscould impair the electrochemical gradient, ionic homeostasis and membrane stability in different regions of the rat brain. These changes may definitely have an adverse impact on CNS and associated functions. However, further approach at the molecular level is in progress which will through more light on this aspect.


The authors are highly thankful to the Head of the Department of Zoology, Director, K. M. Centre for Post Graduate Studies, Puducherry and Research Officer, Department of Pharmacology, Director, Central Siddha Research Institute, Chennai, for providing all the facilities to carry out this work.


All the author have contributed equally




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