ANALYSIS OF THE POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM THE SALIVA AND TONGUE DORSUM TO INHIBIT THE GROWTH OF FUSOBACTERIUM NUCLEATUM

Authors

  • Azzara Nurfitri Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Hedijanti Joenoes Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Boy M Bachtiar Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2017.v9s1.06

Keywords:

Streptococcus salivarius, Fusobacterium nucleatum, Probiotic

Abstract

Objective: Analyzing the potential of S. salivarius isolated from the saliva and tongue dorsum of adults to inhibit the growth of Fusobacterium
nucleatum.
Methods: Polymerase chain reaction, deferred antagonism test, and well-diffused agar test.
Results: Inhibition of the growth F. nucleatum by S. salivarius isolated from the tongue dorsum (p>0.05). No inhibition to the growth of F. nucleatum
by S. salivarius isolated from the saliva. No inhibition to the growth of F. nucleatum by the protein produced by S. salivarius.
Conclusions: The growth of F. nucleatum was not inhibited by S. salivarius isolated from the saliva but by S. salivarius isolated from the dorsum of the
tongue.

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References

Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF,

Sumpradit N, et al. Antibiotic resistance - the need for global solutions.

Lancet Infect Dis 2013;13(12):1057-98.

Horz H, Meinelt A, Houben B, Conrads G. Distribution and persistence

of probiotic Streptococcus salivarius K12 in the human oral cavity as

determined by real-time quantitative polymerase chain reaction. Oral

Microbiol Immunol 2007;22(2):126-30.

Fauzi AA, Shafiei Z, Baharin B, Mohd N. Isolation of lactobacillus

from periodontally healthy subjects and its antimicrobial activity

against periodontal pathogens. Sains Malays 2013;42(1):19-24.

Burton JP, Wescombe PA, Macklaim JM, Chai MH, Macdonald K,

Hale JD, et al. Persistence of the oral probiotic Streptococcus salivarius

M18 is dose dependent and megaplasmid transfer can augment their

bacteriocin production and adhesion characteristics. PLoS One

;8(6):e65991.

Tamura S, Yonezawa H, Motegi M, Nakao R, Yoneda S, Watanabe H,

et al. Inhibiting effects of Streptococcus salivarius on competencestimulating

peptide-dependent biofilm formation by Streptococcus

mutans. Oral Microbiol Immunol 2009;24(2):152-61.

Kaci G, Goudercourt D, Dennin V, Pot B, Doré J, Ehrlich SD, et al.

Anti-inflammatory properties of Streptococcus salivarius, a commensal

bacterium of the oral cavity and digestive tract. Appl Environ Microbiol

;80(3):928-34.

Newman MG, Takei HH, Carranza FA. Carranza’s Clinical

Periodontology. 9th ed. St. Louis, Missouri: Elsevier Saunders; 2002.

Barbour A, Philip K. Variable characteristics of bacteriocin-producing

Streptococcus salivarius strains isolated from Malaysian subjects.

PLoS One 2014;9(6):e100541.

Bowe WP, Filip JC, DiRienzo JM, Volgina A, Margolis DJ. Inhibition

of propionibacterium acnes by bacteriocin-like inhibitory substances

(BLIS) produced by Streptococcus salivarius. J Drugs Dermatol

;5(9):868-70.

Wescombe PA, Upton M, Dierksen KP, Ragland NL, Sivabalan S,

Wirawan RE, et al. Production of the lantibiotic salivaricin A and its

variants by oral streptococci and use of a specific induction assay

to detect their presence in human saliva. Appl Environ Microbiol

;72(2):1459-66.

Prasad S, Morris PC, Hansen R, Meaden PG, Austin B. A novel

bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio

harveyi. Microbiology 2005;151(9):3051-8.

Ogawa A, Furukawa S, Fujita S, Mitobe J, Kawarai T, Narisawa N, et al.

Inhibition of Streptococcus mutans biofilm formation by Streptococcus

salivarius FruA. Appl Environ Microbiol 2011;77(5):1572-80.

Roger P, Delettre J, Bouix M, Béal C. Characterization of Streptococcus

salivarius growth and maintenance in artificial saliva. J Appl Microbiol

;111(3):631-41.

Brown L, Wolf JM, Prados-Rosales R, Casadevall A. Through the wall:

Extracellular vesicles in Gram-positive bacteria, mycobacteria and

fungi. Nat Rev Microbiol 2015;13(10):620-30.

Alakomi HL, Skyttä E, Saarela M, Mattila-Sandholm T, Latva‑Kala K,

Helander IM. Lactic acid permeabilizes gram-negative bacteria

by disrupting the outer membrane. Appl Environ Microbiol

;66(5):2001-5.

MacDonald KW. The Role of Streptococcus salivarius as a Modulator

of Homeostasis in the Oral Cavity. Electronic Thesis and Dissertation

Repository. University of Western Ontario; 2015.

Barbour A, Tagg J, Abou-Zied OK, Philip K. New insights into the

mode of action of the lantibiotic salivaricin B. Sci Rep 2016;6:31749.

Weerkamp AH, McBride BC. Adherence of Streptococcus salivarius

HB and HB-7 to oral surfaces and saliva-coated hydroxyapatite. Infect

Immun 1980;30(1):150-8.

Barbour A, Philip K, Muniandy S. Enhanced production,

purification, characterization and mechanism of action of salivaricin

lantibiotic produced by Streptococcus salivarius NU10. PLoS One

;8(10):e77751.

Draper LA, Cotter PD, Hill C, Ross RP. The two peptide lantibiotic

lacticin 3147 acts synergistically with polymyxin to inhibit Gram

negative bacteria. BMC Microbiol 2013;13:212.

Brötz H, Sahl HG. New insights into the mechanism of action of

lantibiotics - Diverse biological effects by binding to the same

molecular target. J Antimicrob Chemother 2000;46(1):1-6.

Published

30-10-2017

How to Cite

Nurfitri, A., Joenoes, H., & Bachtiar, B. M. (2017). ANALYSIS OF THE POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM THE SALIVA AND TONGUE DORSUM TO INHIBIT THE GROWTH OF FUSOBACTERIUM NUCLEATUM. International Journal of Applied Pharmaceutics, 9, 8–12. https://doi.org/10.22159/ijap.2017.v9s1.06

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