• Sonny Prasetyo Wardojo Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Ratna Meidyawati Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • Munyati Usman Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.



Microleakage, Dentin, Composite, Grape seed extract solution


Objectives: The purpose of this study is to evaluate the influence of grape seed extract (GSE) solution as dentin biomodification on microleakage
between composite resin and dentin.
Methods: A total of 52 dentin specimens were divided into two groups. Group 1 involved the application of GSE solution for 30 seconds after etching.
Group 2 served as the control group.
Results: The GSE solution group significantly increases microleakage between the composite resin and dentin compared to the control group.
Conclusions: The application of GSE solution after etching can increase microleakage between composite resin and dentin.


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Wirsching E. Contemporary options for restoration of anterior teeth

with composite. Quintessence Int 2015;46(6):457-63.

Staehle HJ, Wolff D, Frese C. More conservative dentistry: Clinical

long-term results of direct composite resin restorations. Quintessence

Int 2015;46(5):373-80.

Predigao J, Swift E. Fundamental concepts of enamel and dentin

adhesion. In: Roberson M, Heymann H, Swift E, editors. Studervant’s

Art and Science Operative Dentistry. 5th ed. St. Louis: Mosby Elsevier;

p. 239-61.

Perdigão J, Reis A, Loguercio AD. Dentin adhesion and MMPs: A

comprehensive review. J Esthet Restor Dent 2013;25(4):219-41.

Mazzoni A, Tjäderhane L, Checchi V, Di Lenarda R, Salo T, Tay FR,

et al. Role of dentin MMPs in caries progression and bond stability.

J Dent Res 2015;94(2):241-51.

Rosales-Leal JI. Microleakage of Class V composite restorations

placed with etch-and-rinse and self-etching adhesives before and after

thermocycling. J Adhes Dent 2007;9 Suppl 2:255-9.

Angelina A. Potensi Ekstrak Biji Anggur Sebagai Larutan Irigasi

Saluran Akar Dalam Membersihkan Smear layer (Tesis). Jakarta:

Universitas Indonesia; 2013.

University of Maryland Medical Center (UMMC). APGAR; 2011.

Available from:


Liu Y, Wang Y. Proanthocyanidins’ efficacy in stabilizing dentin

collagen against enzymatic degradation: MALDI-TOF and FTIR

analyses. J Dent 2013;41(6):535-42.

Liu Y, Dusevich V, Wang Y. Addition of grape seed extract

renders phosphoric acid a collagen-stabilizing etchant. J Dent Res


Singla M, Aggarwal V, Kumar N. Effect of chlorhexidine cavity

disinfection on microleakage in cavities restored with composite using

a self-etching single bottle adhesive. J Conserv Dent 2011;14(4):374-7.

Malik S, Bal C, Singh R. Tensile bond strength of total-etch and selfetch

adhesives on moist and dry dentin in-vivo study. Indian J Compr

Dent Care 2014;4:422-7.

Reddy S. Nano composites-synthesis, characterization and industrial

applications. Alex Eng J 2014;6:14-6.

Swapna MU, Koshy S, Kumar A, Nanjappa N, Benjamin S, Nainan

MT. Comparing marginal microleakage of three Bulk Fill composites

in Class II cavities using confocal microscope: An in vitro study.

J Conserv Dent 2015;18(5):409-13.

Nguyen C. A new in vitro method for study of microleakage of dental

restorative material. University of Adelaide Australia; 2013. p. 15-18.

Ahlberg KM, Assavanop P, Tay WM. A comparison of the apical dye

penetration patterns shown by methylene blue and india ink in rootfilled

teeth. Int Endod J 1995;28(1):30-4.

Kalra M, Iqbal K, Nitisusanta LI, Daood U, Sum CP, Fawzy AS. The

effect of proanthocyanidins on the bond strength and durability of resin

sealer to root dentine. Int Endod J 2013;46(2):169-78.

Kaur R, Bak C, Khanna R, Bhullar K, Malhotra S. Effect of pretreatment

with grape seed extract on the bond strength of self etch adhesive.

Indian J Compr Dent Care 2014;4:15-8.

Green B, Yao X, Ganguly A, Xu C, Dusevich V, Walker MP, et al. Grape

seed proanthocyanidins increase collagen biodegradation resistance in

the dentin/adhesive interface when included in an adhesive. J Dent


da Silva NR, Calamia CS, Coelho PG, Carrilho MR, de Carvalho RM,

Caufield P, et al. Effect of 2% iodine disinfecting solution on bond

strength to dentin. J Appl Oral Sci 2006;14(6):399-404.

Liu Y, Wang Y. Effect of proanthocyanidins and photo-initiators on

photo-polymerization of a dental adhesive. J Dent 2013;41(1):71-9.



How to Cite

Wardojo, S. P., Meidyawati, R., & Usman, M. (2017). INFLUENCE OF GRAPE SEED EXTRACT SOLUTION ON MICROLEAKAGE BETWEEN COMPOSITE RESIN AND DENTIN. International Journal of Applied Pharmaceutics, 9, 17–19.



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