EFFICACY OF MOUTH RINSE FORMULATION BASED ON CETYLPYRIDINIUM CHLORIDE 0.1% IN THE CONTROL OF DENTAL CALCULUS BUILDUP
Keywords:Mouth rinse, Cetylpyridinium chloride, Anticalculus
Objective: This study aimed at comparing the antiplaque, anticalculus, and antigingivitis potentials of a mouth rinse containing essential oil, alcohol,
zinc, and fluoride with a mouth rinse containing cetylpyridinium chloride (CPC) 0.1% over 1-, 2-, and 3-month periods.
Methods: This study was a double-blind, parallel randomized clinical trial with a 3-day run-in phase. Respondents were asked to gargle twice daily
with 15 ml of mouth rinse for 30 seconds after brushing teeth. Respondents were 80 females with a mean age of 21 years, and a single dental examiner
was employed throughout the study to decrease the variance. Prophylaxis was performed for all respondents before the intervention. Three mouth
rinses were tested: Group 1 with the mouth rinse containing CPC 0.1%, Group 2 as the negative control, and Group 3 as the positive control with a
mouth rinse containing alcohol. Evaluations were conducted by plaque index, gingival index, calculus index, and CariScreen examinations.
Results: The clinical trial showed that the mouth rinse with alcohol and the mouth rinse containing CPC 0.1% were effective in inhibiting bacterial
buildup (antiplaque) and have anticalculus properties, but with no statistically significant antigingivitis effect.
Conclusion: It was found that the mouth rinse containing alcohol has similar effectiveness with CPC 0.1% mouth rinse, but side effects, such as a
burning sensation, were reported in the alcohol-containing mouth rinse.
Darby I. Non-surgical management of periodontal disease. Aust Dent J
;54 Suppl 1:S86-95.
Bowen DM. Lasers and nonsurgical periodontal therapy. J Dent Hyg
Cortelli SC, Cortelli JR, Shang H, Costa R, Charles CA. Gingival health
benefits of essential-oil and cetylpyridinium chloride mouthrinses: A
-month randomized clinical study. Am J Dent 2014;27(3)119-26.
Table 7: Number of tooth surfaces (n=3291) with calculus measurement results, with categorical statistical approach, which were
yesâ€‘orâ€‘no calculus buildup (surfaceâ€‘level analysis)
Mouth rinse After 1 month After 2 months After 3 months
26 50 83
36 81 116
28 60 86
341 0.011* 0.014*
Table 8: Post hoc analysis of Table 7 in p values from the continuity correction
Mouth rinse After 2 months After 3 months
versus 2 0.005* 0.012*
versus 3 0.371 0.858
versus 3 0.066 0.024*
Table 9: Gingival measurement results
Before After 1 month After 2 months After 3 months pâ€‘values
0 0.04 0.17 0.31 0.000
0 0.08 0.17 0.33 0.000
0 0.17 0.17 0.31 0.000
pâ€‘values 0.132 (Kruskalâ€“Wallis test) 0.437 (Kruskalâ€“Wallis test) 0.469 (Kruskalâ€“Wallis test) 0.934 (1â€‘way ANOVA)
Int J App Pharm, Special Issue (October)
Maharani et al.
Costa X, Laguna E, Herrera D, Serrano J, Alonso B, Sanz M. Efficacy
of a new mouth rinse formulation based on 0.07% cetylpyridinium
chloride in the control of plaque and gingivitis: A 6-month randomized
clinical trial. J Clin Periodontol 2013;40(11):1007-15.
GarcÃa V, Rioboo M, Serrano J, Oâ€™Connor A, Herrera D, Sanz M.
Plaque inhibitory effect of a 0.05% cetyl-pyridinium chloride mouthrinse
in a 4-day non-brushing model. Int J Dent Hyg 2011;9(4):266-73.
Lamont T. Lower concentration of chlorhexidine and cetyl-pyridinium
chloride mouthwash demonstrates some efficacy. Evid Based Dent
Deinzer R, Jahns S, Harnacke D. Establishment of a new marginal
plaque index with high sensitivity for changes in oral hygiene.
J Periodontol 2014;85(12):1730-8.
Gupta D, Jain A. Effect of cinnamon extract and chlorhexidine
gluconate (0.2%) on the clinical level of dental plaque and gingival
health: A 4-week, triple-blind randomized controlled trial. J Int Acad
Fazilat S, Sauerwein R, McLeod J, Finlayson T, Adam E, Engle J,
et al. Application of adenosine triphosphate-driven bioluminescence
for quantification of plaque bacteria and assessment of oral hygiene in
children. Pediatr Dent 2010;32(3):195-204.
Asahi Y, Noiri Y, Miura J, Maezono H, Yamaguchi M, Yamamoto R, et al.
Effects of the tea catechin epigallocatechin gallate on Porphyromonas
gingivalis biofilms. J Appl Microbiol 2014;116(5):1164-71.
Cortelli SC, Cortelli JR, Wu MM, Simmons K, Charles CA. Comparative
antiplaque and antigingivitis efficacy of a multipurpose essential oilcontaining
mouthrinse and a cetylpyridinium chloride-containing
mouthrinse: A 6-month randomized clinical trial. Quintessence Int
Noiri Y, Okami Y, Narimatsu M, Takahashi Y, Kawahara T, Ebisu S.
Effects of chlorhexidine, minocycline, and metronidazole on
Porphyromonas gingivalis strain 381 in biofilms. J Periodontol
Rawlinson A, Pollington S, Walsh TF, Lamb DJ, Marlow I, Haywood J,
et al. Efficacy of two alcohol-free cetylpyridinium chloride
mouthwashes-a randomized double-blind crossover study. J Clin
Sreenivasan PK, Haraszthy VI, Zambon JJ. Antimicrobial efficacy
of 0Â·05% cetylpyridinium chloride mouthrinses. Lett Appl Microbiol
Latimer J, Munday JL, Buzza KM, Forbes S, Sreenivasan PK,
McBain AJ. Antibacterial and anti-biofilm activity of mouthrinses
containing cetylpyridinium chloride and sodium fluoride. BMC
Murata Y, Miyamoto E, Ueda M. Antiplaque activity of some
antimicrobial agents using a simple in vitro method. Caries Res