ANTIBACTERIAL EFFECTS OF 0.1% CHLORINE DIOXIDE ON ACTINOMYCES SP. AS AN AGENT OF BLACK STAIN

Maria Clarissa Eunike; Eva Fauziah; Margaretha Suharsini

doi:10.22159/ijap.2017.v9s2.19

Authors

Maria Clarissa Eunike Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Eva Fauziah Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Margaretha Suharsini Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2017.v9s2.19

Keywords:

Actinomyces sp, Black stain, Bacterial viability, Chlorine dioxide, Chlorhexidine

Abstract

Objective: This study aimed to assess the antibacterial effects of 0.1% chlorine dioxide and 0.1% chlorhexidine mouthrinses on the bacterial viability of Actinomyces sp. as an agent of black stain.

Methods: The authors conducted a clinical trial involving 16 children ages 6â€“11 with at least 8 black-stained teeth. Subjects were randomized into 2 groups and instructed to rinse with chlorine dioxide or chlorhexidine mouthrinse twice daily. At baseline and after 7 days, samples of black stain plaque were collected, and Actinomyces sp. was cultured. Its bacterial viability was evaluated using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay.

Results: After 7 days, Actinomyces sp. viability was remarkably reduced in both groups, and there was a significantly higher reduction in viability in the 0.1% chlorine dioxide group than there was in the 0.1% chlorhexidine group.

Conclusion: Mouthrinse containing 0.1% chlorine dioxide has a greater antibacterial effect against Actinomyces sp. than mouthrinse containing 0.1% chlorhexidine.

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