TOXICITY ANALYSIS OF CRAB SHELL CHITOSAN ARGINYLGLYCYLASPARTIC ACID SCAFFOLD MEMBRANE AND ITS EFFECT ON HUMAN DENTAL PULP CELL VIABILITY

Tri Kurnia Dewi; Mauldina Shabrina; Dewi Fatmasuniarti; Lisa Amir; Erik Idrus

doi:10.22159/ijap.2017.v9s2.40

Authors

Tri Kurnia Dewi Department, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Mauldina Shabrina Department, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Dewi Fatmasuniarti Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Lisa Amir Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Erik Idrus Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2017.v9s2.40

Keywords:

Crab shells, Chitosan, Scaffold, Arginylglycylaspartic acid, Toxicity, Human dental pulp cells

Abstract

Objective: Crab shell chitosan is a biomaterial used for scaffolding. In Indonesia, Badan Tenaga Nuklir Nasional has made a crab shell chitosan arginylglycylaspartic acid (RGD) scaffold membrane. The purpose of adding RGD was to enhance cell attachment to the scaffold. The objective of this research is to analyze the toxicity of crab shell chitosan RGD scaffold membrane on human dental pulp cells and its effect on their viability

Methods: Human dental pulp cells were cultured for 5Â days in Minimum Essential Medium Alpha (Î±-MEM) complete containing amphotericin B, penicillin, streptomycin, and fetal bovine serum. Then, the treatment group was exposed to crab shell chitosan RGD scaffold membrane and crab shell chitosan scaffold membrane incubated for 24 h. The toxicity of the crab shell chitosan RGD scaffold membrane was analyzed with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Result: The result of this research is that crab shell chitosan RGD scaffold membrane did not decrease the percentage of viability of human dental pulp cells.

Conclusion: It is concluded that crab shell chitosan RGD scaffold membrane does not have toxic effects on human dental pulp cells.

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References

Lee M, Li W, Siu RK, Whang J, Zhang X, Soo C, et al. Biomimetic apatite-coated alginate/chitosan microparticles as osteogenic protein carriers. Biomaterials 2009;30:6094-101.

Guleria M, Dua H, Rohila S, Sharma AK. Stem cells in dentistry. Indian J Dent Sci 2014;6:107-12.

Casagrande L, Cordeiro MM, NÃ¶r SA, NÃ¶r JE. Dental pulp stem cells in regenerative dentistry. Odontology 2011;99:1-7.

Nawi NS, Ariffin Z, Alam MK, Noor SN, Hassan A. The assessment of proliferation rate of dental pulp stem cells and stem cell from human exfoliated deciduous teeth by using two different scaffold. Int Med J 2013;20:593-6.

Amir LR, Suniarti DF, Utami S, Abbas B. Chitosan as a potential osteogenic factor compared with dexamethasone in cultured macaque dental pulp stromal cells. Cell Tissue Res 2014;358:407-15.

Bellis SL. Advantages of RGD peptides for directing cell association with biomaterials. Biomaterials 2011;32:4205-10.

Fernandes LL, Resende CX, Tavares DS, Soares GA, Castro LO, Granjeiro JM. Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering. Polimeros 2011;21:1-6.

Tsai WB, Chen YR, Li WT, Lai JY, Liu HL. RGD-conjugated UV-crosslinked chitosan scaffolds inoculated with mesenchymal stem cells for bone tissue engineering. Carbohydr Polym 2012;89:379-87.

Prabaharan M, Sivashankari PR. Prospect of bioactive chitosan-based scaffold in tissue engineering and regenerative medicine. Chitin and Chitosan for Regenerative Medicine. Springer Series on Polymer and Composite Materials In: Dutta P, editor. New Delhi: Springer; 2016. p. 47-57.

Chen YL, Lee HP, Chan HY, Sung LY, Chen HC, Hu YC, et al. Composite chondroitin-6-sulfate/dermatan sulfate/chitosan scaffolds for cartilage tissue engineering. Biomaterials 2007;28:2294-305.