• Madhumithra S. K. Department of Biomedical Engineering, SSN College of Engineering, Kalavakkam, Chennai, 603110, Tamil Nadu, India
  • Balashanmugam P. Centre for Human & Organisational Resources Development (CHORD), CSIR-Central Leather Research Institute, Chennai, 600020, Tamil Nadu, India
  • Mosachristas K. Avanz Bio Pvt. Ltd., Ganthipuram, East Tambaram, Chennai, 600059, Tamil Nadu, India
  • Tamil Selvi A. Centre for Human & Organisational Resources Development (CHORD), CSIR-Central Leather Research Institute, Chennai, 600020, Tamil Nadu, India
  • Subashini R. Department of Biomedical Engineering, SSN College of Engineering, Kalavakkam, Chennai, 603110, Tamil Nadu, India



Gold nanoparticles, Pistacia vera, Cytotoxicity, Apoptosis, Ovarian cancer cell lines, Fluorescence microscopy


Objective: To synthesize the gold nanoparticles by a biological method using the extract obtained from the shells of Pistacia vera (P. vera) and to study its effective role in the anticancer activity.

Methods: The synthesis of gold nanoparticles using the extract obtained from the shells of Pistacia vera was confirmed by the color change and substantiating the same using ultraviolet (UV) visible spectroscopy. The size and the shape of the particles were studied using field emission scanning electron microscopy (FESEM). The stability of the nanoparticles was assessed by using the UV visible spectroscopy and Fourier-transform infrared spectroscopy (FTIR). The anticancer activity of the gold nanoparticles on the cancer cell lines was studied on PA1 ovarian cancer cell lines using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nature of cell death was analyzed using the fluorescence microscopy.

Results: The ruby red color confirmed the formation of gold nanoparticles and it was substantiated by the absorption peak at 543.2 nm in the UV visible spectroscopy. The gold nanoparticles synthesized from the Pistacia vera shell showed the spherical shape and were in the size of around 10-40 nm when analyzed with FESEM. The different functional groups were indicated in the FTIR spectra which were consisting of phenol, alcohol, alkenes and aromatics.

Conclusion: The synthesis of the gold nanoparticle using the extract obtained from the shells of Pistacia vera has effective anticancer activity.


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Kadian R. Nanoparticles: a promising drug delivery approach. Asian J Pharm Clin Res 2018;11:30-5.

Pandey P, Dahiya M. A brief review on inorganic nanoparticles. J Crit Rev 2014;3:18-26.

Bastús NG, Comenge J, Puntes V. Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus Ostwald ripening. Langmuir. 2011;27:11098-105.

Grzelczak M, Perez-Juste J, Mulvaney P, Liz-Marzan LM. Shape control in gold nanoparticle synthesis. Chem Soc Rev 2008;37:1783-91.

Kumar P, Roy I. Applications of gold nanoparticles in clinical medicine. Int J Pharm Pharm Sci 2016;8:11-6.

Zhang X. Gold nanoparticles: recent advances in the biomedical applications. Cell Biochem Biophys 2015;72:771-5.

Chen YS, Hung YC, Liau I, Huang GS. Assessment of the in vivo toxicity of gold nanoparticles. Nanoscale Res Lett 2009;4:858-64.

Antony E, Sathiavelu M, Arunachalam S. Synthesis of silver nanoparticles from the medicinal plant Bauhinia acuminata and biophytum sensitivum–a comparative study of its biological activities with plant extract. Int J Appl Pharm 2017;9:22-9.

Pantidos N, Horsfall LE. Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. J Nanomed Nanotechnol 2014;5:1-10.

Singh P, Kim YJ, Zhang D, Yang DC. Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol 2016;34:588-99.

Dykman L, Khlebtsov N. Gold nanoparticles in biomedical applications: recent advances and perspectives. Chem Soc Rev 2012;41:2256-82.

Elia P, Zach R, Hazan S, Kolusheva S, Porat ZE, Zeiri Y. Green synthesis of gold nanoparticles using plant extracts as reducing agents. Int J Nanomed 2014;9:4007-21.

Bozorgi M, Memariani Z, Mobli M, Salehi Surmaghi MH, Shams-Ardekani MR, Rahimi R. Five pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): a review of their traditional uses, phytochemistry, and pharmacology. Sci World J 2013;1-33.

Kashaninejad M, Mortazavi A, Safekordi A, Tabil LG. Some physical properties of pistachio (Pistacia vera L.) nut and its kernel. J Food Eng 2006;72:30-8.

Roitman JN, Merrill GB, Beck JJ. Survey of ex situ fruit and leaf volatiles from several Pistacia cultivars grown in California. J Sci Food Agric 2011;91:934-42.

Goli AH, Barzegar M, Sahari MA. Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chem 2005;92:521-5.

Almehdar H, Abdallah HM, Osman AM, Abdel Sattar EA. In vitro cytotoxic screening of selected Saudi medicinal plants. J Nat Med 2012;66:406-12.

Mubarak Ali D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B 2011;85:360-5.

Balashanmugam P, Durai P, Balakumaran MD, Kalaichelvan PT. Phytosynthesized gold nanoparticles from C. roxburghii DC. leaf and their toxic effects on normal and cancer cell lines. J Photochem Photobiol B 2016;165:163-73.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Singh AK, Srivastava ON. One-step green synthesis of gold nanoparticles using black cardamom and effect of pH on its synthesis. Nanoscale Res Lett 2015;10:353-65.

Verma HN, Singh P, Chavan RM. Gold nanoparticle: synthesis and characterization. Vet World 2014;7:72-7.

Jain PK, Lee KS, El-Sayed IH, El-Sayed MA. Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. J Phys Chem B 2006;110:7238-48.

Kasthuri J, Veerapandian S, Rajendiran N. Biological synthesis of silver and gold nanoparticles using apiin as reducing agent. Colloids Surf B 2009;68:55-60.

Jeyaraj M, Rajesh M, Arun R, Mubarak Ali D, Sathishkumar G, Sivanandhan G, et al. An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells. Colloids Surf B 2013;102:708-17.

Castro L, Blazquez ML, Munoz JA, Gonzalez F, Ballester A. Biological synthesis of metallic nanoparticles using algae. IET Nanobiotechnol 2013;7:109-16.

Taha A, Shamsuddin M. Biosynthesis of gold nanoparticles using Psidium guajava leaf extract. Malaysian J Fundamental Appl Sci 2013;9:119-22.

Dwivedi AD, Gopal K. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloids Surf A 2010;369:27-33.

Demiral Ä°, Atilgan NG, Åžensoz S. Production of biofuel from soft shell of pistachio (Pistacia vera L.). Chem Eng Commun 2008;196:104-15.

Açıkalın K, Karaca F, Bolat E. Pyrolysis of pistachio shell: effects of pyrolysis conditions and analysis of products. Fuel 2012;95:169-77.

Preethi R, Padma PR. Anticancer activity of silver nanobioconjugates synthesized from piper betle leaves extract and its active compound eugenol. Int J Pharm Pharm Sci 2016;8:201-5.

Ashwini S, Suresh Babut V, Saritha, Manjula Shantaram. Seaweed extracts exhibit anticancer activity against HeLa cell lines. Int J Curr Pharm Res 2017;9:114-7.

Singh N, Ranjan V, Deeba Zaidi HS, Singh A, Lodha D, Sharma R, et al. Insulin catalyzes the curcumin-induced wound healing: an in vitro model for gingival repair. Indian J Pharmacol 2012;44:458-62.

Preethi R, Padma PR. Green synthesis of silver nanobioconjugates from Piper betle leaves and its anticancer activity on A549 cells. Asian J Pharm Clin Res 2016;9:252-7.

Baskić D, Popovic S, Ristic P, Arsenijevic NN. Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biol Int 2006;30:924-32.

Curcic MG, Stankovic MS, Mrkalic EM, Matovic ZD, Bankovic DD, Cvetkovic DM, et al. Antiproliferative and proapoptotic activities of methanolic extracts from Ligustrum vulgare L. as an individual treatment and in combination with palladium complex. Int J Mol Sci 2012;13:2521-34



How to Cite

K., M. S., P., B., K., M., A., T. S., & R., S. (2018). IN VITRO CYTOTOXICITY OF BIOSYNTHESIZED GOLD NANOPARTICLES FROM SHELLS OF PISTACIA VERA L. International Journal of Applied Pharmaceutics, 10(4), 162–167.



Original Article(s)