IN VITRO CYTOTOXICITY OF BIOSYNTHESIZED GOLD NANOPARTICLES FROM SHELLS OF PISTACIA VERA L
Keywords:Gold nanoparticles, Pistacia vera, Cytotoxicity, Apoptosis, Ovarian cancer cell lines, Fluorescence microscopy
Objective: To synthesize the gold nanoparticles by a biological method using the extract obtained from the shells of Pistacia vera (P. vera) and to study its effective role in the anticancer activity.
Methods: The synthesis of gold nanoparticles using the extract obtained from the shells of Pistacia vera was confirmed by the color change and substantiating the same using ultraviolet (UV) visible spectroscopy. The size and the shape of the particles were studied using field emission scanning electron microscopy (FESEM). The stability of the nanoparticles was assessed by using the UV visible spectroscopy and Fourier-transform infrared spectroscopy (FTIR). The anticancer activity of the gold nanoparticles on the cancer cell lines was studied on PA1 ovarian cancer cell lines using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nature of cell death was analyzed using the fluorescence microscopy.
Results: The ruby red color confirmed the formation of gold nanoparticles and it was substantiated by the absorption peak at 543.2 nm in the UV visible spectroscopy. The gold nanoparticles synthesized from the Pistacia vera shell showed the spherical shape and were in the size of around 10-40 nm when analyzed with FESEM. The different functional groups were indicated in the FTIR spectra which were consisting of phenol, alcohol, alkenes and aromatics.
Conclusion: The synthesis of the gold nanoparticle using the extract obtained from the shells of Pistacia vera has effective anticancer activity.
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