PREPARATION AND CHARACTERIZATION OF EDIBLE OIL NANOEMULSIONS FOR ENHANCED STABILITY AND ORAL DELIVERY OF CURCUMIN
DOI:
https://doi.org/10.22159/ijap.2018v10i6.28726Keywords:
Edible oil nanoemulsions, Nanoemulsion, Olive oil emulsions, Curcumin delivery system, Oral bioavailability, Release kineticsAbstract
Objective: This work aims to improve the oral bioavailability and long-term aqueous stability of curcumin using various edible oil nanoemulsions (NEs).
Methods: NEs were optimized using the water titration method. Curcumin was loaded into optimized emulsions, and the physicochemical characteristics were determined. Long-term stability of curcumin in the edible oil NEs was analyzed by determining the droplet size, PDI and curcumin concentrations over 4 mo. Release of curcumin from the NEs was determined using a Franz diffusion apparatus and analysed using 5 mathematical models.
Results: The absorbance of curcumin was linear over the concentration range of 1 to 10 μg. ml-1. The LOD and LOQ ranged from 0.57 to 1.26μg. ml-1 and 1.89 to 4.19μg. ml-1 respectively. All the NEs were monodisperse and had a droplet size less than 150 nm. Long-term emulsion stability shows no change in droplet size and PI (Dunnett's multiple comparisons test with a confidence interval of 95%). Olive oil NE showed significantly low release in gastric fluid (9.28%) with a good release (92.99%) in intestinal fluid and 48% in a body fluid by 8 h.
Conclusion: The work highlights the use of olive oil NEs as a delivery vehicle for curcumin with excellent release characteristics and the ability to protect curcumin in an aqueous environment.
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Sari T, B Mann, R Kumar, R Singh, R Sharma, M Bhardwaj, et al. Preparation and characterization of nanoemulsion encapsulating curcumin. Food Hydrocoll 2015;43:540-6.
Chainani Wu N. Safety and anti-inflammatory activity of curcumin: a component of tumeric (Curcuma longa). J Altern Complement Med 2003;9:161-8.
Sharma O. Antioxidant activity of curcumin and related compounds. ‎Biochem Pharmacol 1976;25:1811-2.
Ravindran J, S Prasad, BB Aggarwal. Curcumin and cancer cells: how many ways can curry kill tumor cells selectively? AAPS J 2009;11:495-510.
Basniwal RK, HS Buttar, V Jain, N Jain. Curcumin nanoparticles: preparation, characterization, and antimicrobial study. J Agric Food Chem 2011;59:2056-61.
Mishra S, K Palanivelu. The effect of curcumin (turmeric) on Alzheimer's disease: an overview. Ann Indian Acad Neurol 2008;11:13.
Wongcharoen W, A Phrommintikul. The protective role of curcumin in cardiovascular diseases. Int J Cardiol Heart Vasc 2009;133:145-51.
Anand P, AB Kunnumakkara, RA Newman, BB Aggarwal. Bioavailability of curcumin: problems and promises. Mol Pharm 2007;4:807-18.
Bisht S, G Feldmann, S Soni, R Ravi, C Karikar, A Maitra, et al. Polymeric nanoparticle-encapsulated curcumin ("nanocurcumin"): a novel strategy for human cancer therapy. J Nanobiotechnol 2007;5:3.
Kurzrock R, L Li. Liposome-encapsulated curcumin: in vitro and in vivo effects on proliferation, apoptosis, signaling, and angiogenesis. J Clin Oncol 2005;23:4091.
Athira G, A Jyothi. Preparation and characterization of curcumin loaded cassava starch nanoparticles with improved cellular absorption. Int J Pharm Pharm Sci 2014;6:171-6.
Liu A, H Lou, L Zhao, P Fan. Validated LC/MS/MS assay for curcumin and tetrahydrocurcumin in rat plasma and application to the pharmacokinetic study of phospholipid complex of curcumin. J Pharm Biomed Anal 2006;40:720-7.
John V, G Kuttan, K Krishnankutty. Anti-tumor studies of metal chelates of synthetic curcuminoids. J Exp Clin Cancer Res 2002;21:219-24.
McClements DJ, Y Li. Structured emulsion-based delivery systems: controlling the digestion and release of lipophilic food components. Adv Colloid Interface Sci 2010;159:213-28.
Lovelyn C, AA Attama. Current state of nanoemulsions in drug delivery. J Biomater Nanobiotechnol 2011;2:626-39.
Constantinides PP, JP Scalart. Formulation and physical characterization of water-in-oil microemulsions containing long-versus medium-chain glycerides. Int J Pharm 1997;158:57-68.
Marques MR, R Loebenberg, M Almukainzi. Simulated biological fluids with possible application in dissolution testing. Dissolution Technol 2011;18:15-28.
Gupta A, HB Eral, TA Hatton, PS Doyle. Nanoemulsions: formation, properties, and applications. Soft Matter 2016; 12:2826-41.
Delmas T, H Piraux, AC Couffin, I Texier, FO Vinet, P Poulin, et al. How to prepare and stabilize very small nanoemulsions. Langmuir 2011;27:1683-92.
Nasr A, A Gardouh, M Ghorab. Effect of oils, surfactants and cosurfactants on phase behavior and physicochemical properties of self-nanoemulsifying drug delivery system (SNEDDS) for irbesartan and olmesartan. Int J Appl Pharm 2016;8:1-9.
Al-Adham I, A Al-Nawajeh, E Khalil, P Collier. The antimicrobial activity of oil-in-water microemulsions is predicted by their position within the microemulsion stability zone. Int Arab J Antimicrob Agents 2012;2:1-8.
Waterman E, B Lockwood. Active components and clinical applications of olive oil. Altern Med Rev 2007;12:331-43.
Zheng B, Z Zhang, F Chen, X Luo, DJ McClements. Impact of delivery system type on curcumin stability: comparison of curcumin degradation in aqueous solutions, emulsions, and hydrogel beads. Food Hydrocoll 2017;71:187-97.
Shelat P, VK Mandowara, DG Gupta, S Patel. Formulation of curcuminoid loaded solid lipid nanoparticles in order to improve oral bioavailability. Int J Pharm Pharm Sci 2015;7:278-82.
Sutradhar KB, M Amin. Nanoemulsions: increasing possibilities in drug delivery. Eur J Nanomed 2013;5:97-110.
Dash S, PN Murthy, L Nath, P Chowdhury. Kinetic modeling on drug release from controlled drug delivery systems. Acta Pol Pharm 2010;67:217-23.
Gouda R, H Baishya, Z Qing. Application of mathematical models in drug release kinetics of carbidopa and levodopa ER tablets. J Development Drugs 2017;6:1-8.
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