ANALYSIS OF SYNTHETIC RED DYES IN RED SPINACH SAMPLES (AMARANTHUS TRICOLOR L.)
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.04Keywords:
Amaranth, Karmoisin, Paper chromatography, Ponceau 4R, Red spinach, Rhodamin B, Thin-layer chromatography-densitometry, thinlayer chromatographyAbstract
Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples.
Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by
paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol–
ethanol–water (4:5:5) and isobutanol–ethanol–water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol–ethanol–water (3:7:1).
Results: No synthetic red dyes were found in the seven red spinach samples.
Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.
Downloads
References
Cahyadi W. Analysis and Health Aspects of Food Additives. 2nd ed.
Jakarta: Penerbit Bumi Aksara; 2008. p. 61-5.
Fig. 1: Differences of pH and temperature between experiments performed on different days
The 2nd Physics and Technologies in Medicine and Dentistry Symposium (PTMDS), Universitas Indonesia. Depok, Indonesia 18
Int J App Pharm, Vol 10, Special Issue 1, 2018
Mudjajanto ES, Pertanyaan H. Eddy Setyo Mudjajanto. Food and
Nutrition Technology Observer. Bogor: Harian Pagi Jurnal Bogor;
Baliwati YF, Khosman A, Dwiriana CM. Introduction to Food and
Nutrition. Jakarta: Penerbit Penebar Swadaya; 2004. p. 90-1.
Neumann HG. Aromatic amines: Mechanisms of carcinogenesis
and implications for risk assessment. Front Biosci (Landmark Ed)
;15:1119-30.
Brüschweiler BJ, Merlot C. Azo dyes in clothing textiles can be cleaved
into a series of mutagenic aromatic amines which are not regulated yet.
Regul Toxicol Pharmacol 2017;88:214-26.
Mahindru SN. Food Additives: Characteristics, Detection and
Estimation. New Delhi: Tata McGraw-Hill Publishing Company; 2000.
p. 91-105.
National Standardization Agency. Methods for Food Coloring Testing.
Jakarta: National Standardization Agency; 1992.
Ozdemir H. Dyeing properties of natural dyes extracted from the junipers
leaves (J. excelsa Bieb. and J. oxycedrus L.). J Nat Fiber 2017;14:134-42.
Ghouila H, Meksi N, Haddar W, Mhenni MF, Jannet HB. Extraction,
identification and dyeing studies of isosalipurposide, a natural chalcone
dye from Acacia cyanophylla flowers on wool. Industr Crops Prod
;35:31
Published
How to Cite
Issue
Section
