ANALYSIS OF BISPHENOL A IN INDONESIAN CANNED FOOD BY GAS CHROMATOGRAPHY
Keywords:Bisphenol A, Canned foods, Contamination analysis, Gas chromatography-flame ionization detection, Validation
Objective: This study aimed to design and optimize a gas chromatography-flame ionization detection (GC-FID) method to determine the bisphenol A (BPA)
content in Indonesian canned food samples.
Methods: GC with Hewlett-Packard-1 capillary columns (length, 30 m; inside diameter, 0.25 mm; and film thickness, 0.25 Î¼m) was used with a column
temperature of 150Â°C that was programmed to increase by 10Â°C/min to 260Â°C. Injector and detector temperatures were 280 and 300Â°C, respectively,
the gas flow rate was 1.0 mL/min, and injection volume was 3.0 Î¼L. Three types of canned food samples were prepared by ethyl acetate extraction and
stored under four different conditions (4â€“8Â°C, 25â€“30Â°C, 40Â°C for 30 min, and 40Â°C for 60 min) to determine BPA migration levels.
Results: Method validation (system compatibility, selectivity, calibration curve linearity, accuracy, and precision) was acceptable for BPA
concentrations ranging from 2 to 15 Î¼g/mL, with a coefficient of correlation of 0.99983. The limits of detection and quantitation were 0.287 and
0.956 Î¼g/mL, respectively. Only one canned food sample type (Group A) showed BPA contamination under all storage conditions and exceeded the
recommended guidelines for daily ingestion.
Conclusion: The optimized GC-FID method was selective and relatively sensitive in the detection and quantitation of BPA. Furthermore, higher
storage temperatures and durations increased the level of BPA migration into food.
Food and Drug Supervisory Agency of the Republic of Indonesia.
Bisphenol A (BPA) on Food Packaging. Jakarta: Food and Drug
Supervisory Agency of the Republic of Indonesia; 2014.
U.S Department of Health and Human Sciences. NTP-CERHR
Monograph on the Potential Human Reproductive and Developmental
Effects of bisphenol A. North Carolina: US State U.S Department of
Health and Human Sciences; 2008.
Rykowska I, Wasiak W. Properties, threats, and methods of analysis of
bisphenol A and its derivatives. Acta Chromatogr 2006;16:7-27.
Shrinithivihahshini N, Mahamuni D, Praveen N. Bisphenol A migration
study in baby feeding bottles of selected brands available in the Indian
market. Curr Sci 2014;25:1081-4.
Dutta M, Paul G. Bisphenol A dose-and time-dependently induces
oxidative stress in rat liver mitochondria vivo. Asian J Pharm Clin Res
Indonesian Ministry of Trade. Indonesian Production and Tradeâ€“
Indonesia Technical Requirements Information System (INATRIMS);
Available from: http://www.inatrims.kemendag.go.id/id/product/
accessed on 2017 Jan 23].
Poole CF. Ionization-based detectors for gas chromatography. J
Chromatogr A 2015;1421:137-53.
Obreshkova D, Tsvetkova DD, Ivanov K. Simultaneous identification
and determination of total content of aminoacids in food supplementstablets
by gas chromatography. Asian J Pharm Clin Res 2012;5:57-68.
Preet R, Gupta RC. Determination of fatty acids in Solanum surattense
burm.F. By using gas chromatography. Asian J Pharm Clin Res
Gandjar IG, Rohman A. Chemical Pharmacy Analysis. Yogyakarta:
Pustaka Pelajar; 2007. p. 463-80.
Farajzadeh M, Abbaspour M, Mogaddam M, Ghorbanpour H. Determination
of some synthetic phenolic antioxidants and bisphenol A in honey using
dispersive liquid-liquid microextraction followed by gas chromatograhyflame
ionization detection. Food Anal Methods 2015;8:20135-43.
Zuo Y, Zhu Z. Simultaneous identification and quantification of
-cumylphenol, 2,4-bis-(dimethylbenzyl)phenol and bisphenol A in
prawn Macrobrachium rosenbergii. Chemosphere 2014;107:447-53.