EFFICACY OF IONIC LIQUID [MIM]BR-BASED MAE ON RESVERATROL AND PHENOLIC COMPOUNDS EXTRACTION FROM GNETUM GNEMON SEEDS AND THEIR DPP-4 INHIBITORY ACTIVITY
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.36Keywords:
Gnetum gnemon, [bmim]Br, Trans-resveratrol, Microwave-assisted extraction, Dipeptidyl peptidase-4, Phenolic contentAbstract
Objective: This study aimed to determine the inhibitory activity of 1-butyl-3-methylimidazolium bromide ([bmim]Br) extracts of melinjo seeds
(Gnetum gnemon) on dipeptidyl peptidase-4 (DPP-4).
Methods: Melinjo seeds were extracted by a [bmim]Br microwave-assisted method using various extraction parameters and the inhibitory activity
of DPP-4 of all extracts was determined in 96-well microplates using Cayman inhibitor screening assay. Determination of trans-resveratrol content
was conducted using a reverse-phase high-performance liquid chromatography method. The total phenolic content was determined using a 96-well
microplate reader. Data analysis for the determination of the optimal extraction conditions was developed by response surface methodology.
Results: The extract obtained from the third run showed the highest inhibition (28.73%) against DPP-4 activity with the total phenolic content of
1.96 mg gallic acid equivalent/g the seed powder.
Conclusion: The analytical results revealed the following optimal conditions: Solvent concentration 1.5 M, liquid-solid ratio 23:1, and extraction time
15 min.
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