PHARMACOKINETIC PROFILE OF METFORMIN HYDROCHLORIDE IN DRIED BLOOD SPOT OF HEALTHY SUBJECTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYâ€“PHOTODIODE ARRAY
Keywords:Dried blood spot, High-performance liquid chromatography, Metformin hydrochloride, Pharmacokinetic profile, Validation
Objective: This study aimed to analyze the metformin hydrochloride in dried blood spot (DBS) sample and evaluate a pharmacokinetic profile in six
healthy subjects who administered 850-mg metformin hydrochloride in tablet using high-performance liquid chromatographyâ€“photodiode array
Methods: Metformin analysis was performed on data of six healthy subjects who administered 850 mg of metformin hydrochloride as tablets. Blood
samples were taken at 12-time points at intervals of up to 12 h, and data were analyzed using HPLCâ€“PDA.
Results: A linear calibration curve was obtained in the range of 25â€“5000 ng/mL, with r=0.9990. Pharmacokinetic profiles of metformin were obtained
using DBS samples from six healthy participants, with a Cmax of 347.3â€“416.22 ng/mL. The average tmax and t1/2 were obtained at 3 and 2 h, respectively,
and the area under the curve (AUC)0-t/AUC0-âˆž ratio was >80% for all subjects.
Conclusion: The DBS biosampling technique can be developed for application in pharmacokinetic study of metformin HCl.
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