• LIUDMYLA SLOBODIANIUK Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001, Ternopil, Ukraine
  • LILIIA BUDNIAK Department of Pharmacy Management, Economics and Technology, І. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001, Ternopil, Ukraine
  • SVITLANA MARCHYSHYN Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001, Ternopil, Ukraine
  • ROXOLANA BASARABA Department of Pharmacy, Bukovinian State Medical University, Theatralna sq. 2, 58002, Chernivtsi, Ukraine



Antennaria dioica Gaertn, Polysaccharides, Monosaccharides, Amino acids, GCMS, HPLC


Objective: The purpose of our study was to determine sugars and amino acids content of Antennaria dioica Gaertn. herb. In continuation of the investigation of biologically active substances from Antennaria dioica Gaertn., it advisable to study the qualitative composition and quantitative contents of sugars and amino acids from the herb of this plant.

Methods: The herb of Antennaria dioica Gaertn. was analyzed for the content of sugars by GC/MS. The amino acids were identified and quantified by HPLC method.

Results: The results of GC/MS analysis showed that in Antennaria dioica Gaertn. herb D-glucose had the highest content (7.16±0.09) mg/g, followed by D-fructose (5.27±0.06) mg/g and D-saccharose (6.72±0.08) mg/g. In the raw material a large amount of monosaccharides derivative–Myo-inositol was revealed, a content of which was (2.12±0.06) mg/g. We determined 17 bound and 16 free amino acids in the Antennaria dioica Gaertn. by HPLC method.

Conclusion: The contents of primary metabolites provide opportunities for creating medicine and food supplements. The results show that Antennaria dioica Gaertn. is a rich source of these important biologically active substances. The resulting data will be used with the further purpose to produce new drugs of natural origin.


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Stoiko L, Kurylo Khr. Development of optimal technology of alcohol extract Centaurium erythraea Rafn. herb. Arch Balkan Med Union 2018;53:523-8.

Babota M, Mocan A, Vlase L, Cris O, Ielciu I, Gheldiu AM, et al. Phytochemical analysis, antioxidant and antimicrobial activities of Helichrysum arenarium (L.) moench. and Antennaria dioica (L.) gaertn. flowers. Molecules 2018;23:409.

Erbar C, Leins P. Diversity of styles and mechanisms of secondary pollen presentation in basal asteraceae-new insights in phylogeny and function. Flora Morphol Distrib Funct Ecol Plants 2015;217:109-30.

Bohm BA, Stuessy TF. Flavonoids of the sunflower family (Asteraceae) springer-verlag wien; New York, USA: Introduction to the Sunflower Family; 2001. p. 1.

Garcia Herrera P, Sanchez Mata MC, Camara M, Fernandez Ruiz V, Diez Marques C, Molina M, et al. Nutrient composition of six wild edible mediterranean Asteraceae plants of dietary interest. J Food Compos Anal 2014;34:163–70.

Bessada SMF, Barreira JCM, Oliveira MBPP. Asteraceae species with most prominent bioactivity and their potential applications. A Rev Ind Crops Prod 2015;76:604–15.

Bayer RJ. Antennaria. Flora of North America Editorial Committee. New York: Oxford University Press; 2006. p. 388.

Marchyshyn S, Basaraba R, Berdey T. Investigation of phenolic compounds of Antennaria dioica (L.) Gaertn. herb. Pharma Innov J 2017;6:09-11.

Semeniv DV, Belik GV. The experience of Antennaria dioica application in folk medicine and prospects of this plant use for creation of new phytohemostatics. Ukr Biopharm J 2016;6:37-41.

Mericli AH. Constituents of Antennaria dioica. J Nat Prod 1983;46:941.

Coady Y, Boylan F. Ethnopharmacology in Ireland: an overview. Braz J Pharmacogn 2014;24:197-205.

Abad MJ, Bedoya LM, Bermejo P. Fighting multidrug resistance with herbal extracts, essential oils and their components. Academic Press: San Diego, USA; 2013. p. 205-19.

Husak L, Dakhym I, Marchyshyn S, Nakonechna S. Determination of sugars and fructans content in Stachys sieboldii. Int J Green Pharm 2018;12:70-4.

Chen Y, Xie MY, Wang YX, Nie SP, Li C. Analysis of the monosaccharide composition of purified polysaccharides in ganoderma atrum by capillary gas chromatography. J Phytochem Anal 2009;20:503-10.

Kozachok S, Marchyshyn S, Ostapchuk A, Zavyalova L. Monosaccharide composition of Herniaria glabra L. and Herniaria polygama. J Gay Curr Issues Pharm Med Sci 2016;29:142-4.

Jámbor A, Molnar Perl I. Quantitation of amino acids in plasma by high performance liquid chromatography: simultaneous deproteinization and derivatization with 9-fluorenylmethyloxycarbonyl chloride. J Chromatogr A 2009; 1216:6218-23.

Hanczko R, Jambor A, Perl A, Molnar Perl I. Advances in the ophthalaldehyde derivatizations: comeback to the o-phthalaldehydeethanethiol reagent. J Chromatogr A 2007;1163:25-42.

Jámbor A, Molnar Perl I. Amino acid analysis by high-performance liquid chromatography after derivatization with 9-fluorenylmethyloxycarbonyl chloride literature overview and further study. J Chromatogr A 2009;1216:3064-77.

Stoiko L, Dakhym I, Pokotylo O, Marchyshyn S. Polysaccharides in Centaurium erythraea Rafn. Int J Res Ayurveda Pharm 2017;8:252-5.

Mergenthaler P, Lindauer U, Dienel GA, Meisel A. Sugar for the brain: the role of glucose in physiological and pathological brain function. Tren Neur 2013;36:587-97.

Costantino D, Minozzi G, Minozzi F, Guaraldi C. Metabolic and hormonal effects of myo-inositol in women with polycystic ovary syndrome: a double-blind trial. Eur Rev Med Pharmacol Sci 2009;13:105-10.

Stanford Kr I, Takahashi H, So K, Alves Wagner AB, Prince NB, Lehnig AC, et al. Maternal exercise improves glucose tolerance in female offspring. Diabetes 2017;66:2124-36.

Yoshino J, Conte C, Fontana L, Mittendorfer B, Imai Sh, Schechtman KB, et al. Resveratrol supplementation does not improve metabolic function in non-obese women with normal glucose tolerance. Cell Metab 2012;16:658-64.

Szabados L, Savoure A. Proline: a multifunctional amino acid. Trends Plant Sci 2009;15:89-97.

Verbruggen N, Hermans C. Proline accumulation in plants: a review. Amino Acids 2008;35:753-59.

Moran Palacio EF, Tortoledo Ortiz O, Yanez Farias GA, Zamora Alvarez LA, Stephens Camacho NA, Sonanez Organis JG, et al. Determination of amino acids in medicinal plants from southern sonora, mexico. Trop J Pharm Res 2014;13:601-6.

Wu G. Amino acids: metabolism, functions, and nutrition. Amino Acids 2009;37:1-17.

Rhoads MJ, Wu G. Glutamine, arginine, and leucine signaling in the intestine. Amino Acids 2009;37:111-22.

Karpyuk UV, Kislichenko VS, Gureva IG. HPLC determination of free and bound amino acids in Bryonia alba. Chem Nat Comp 2015;51:399-400.

Karpiuk UV, Abudayeh ZHM, Kyslychenko VS, Yemelianova OІ. Isolation and identification of some primary metabolites, micro-and macroelements of Aesculus hippocastanum L. seeds. Intern J Pharm Phytochem Res 2017;9:108-13.

Radovick S, Margaret H, MacGillivray. Pediatric endocrinology: a practical clinical guide, Second Edition Contemporary Endocrinology. 2d ed. Springer Science and Business Media; 2013. p. 624.



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