• ADANG FIRMANSYAH School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia, Sekolah Tinggi Farmasi Indonesia, Bandung, Indonesia
  • ILMA NUGRAHANI School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
  • KOMAR RUSLAN WIRASUTISNA School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
  • SLAMET IBRAHIM School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia



Boron-silica based mesoporous, Curcuminoid, Isolation, Turmeric extract


Objective: The purpose of this study was to develop the isolation method for curcuminoid from turmeric extract using boron-silica based mesoporous as an adsorbent.

Methods: The formation of mesoporous materials were conducted using the sol-gel technique. The characterization of mesoporous materials was analyzed using a scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier transforms infrared spectrometry (FTIR). The extraction of turmeric was done by solvent extraction using ethanol 96 %. The isolation of curcuminoid was achieved by the adsorption method using mesoporous materials, both for silica-based mesoporous (MCM) and boron-silica based mesoporous (BMCM). The elution of curcuminoid-loaded mesoporous was conducted using various solvents. The concentration of total curcuminoid and its compounds was measured by visible spectrometry and high-performance liquid chromatography (HPLC).

Results: Morphology of MCM and BMCM shows the homogenous regular spherical shape, but having a different size. MCM has a smaller diameter particle size (500-600 nm) compared to BMCM (700-900 nm). On the other hand, BMCM has a smaller pore size (1-5 nm) compared to MCM (5-20 nm). The FTIR spectra of BMCM shows the additional vibration at 1400-1600 cm for B-O-H bond. Visible spectrometry measurement shows that the highest concentration of curcuminoid eluted from BMCM is 65.411±0.056 ppm by using ethyl acetate as a solvent, while the concentration of curcuminoid eluted from MCM is 11.503±0.054 ppm by using the same solvent. The results of curcuminoid adsorption and elution, indicating that ethyl acetate is the best solvent to elute curcuminoid due to its 98.83 % purity using HPLC analysis.

Conclusion: It was concluded that boron-silica based mesoporous showed stronger curcuminoid adsorption than silica-based mesoporous therefore found to be a potential adsorbent for curcuminoid isolation from turmeric extract.


Download data is not yet available.


Jurenka JS. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev 2009;14:141-53.

Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci 2008;65:1631-52.

Moghadamtousi SZ, Kadir HA, Hassandarvish P, Tajik H, Abubakar S, Zandi KA. Review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed Res Int 2014;2014:1-12.

Jayaprakasha GK, Rao LJM, Sakariah KK. Improved HPLC method for the determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin. J Agric Food Chem 2002;50:3668-72.

Braga MEM, Leal PF, Carvalho JE, Meireles MAA. Comparison of yield, composition, and antioxidant activity of turmeric (Curcuma longa L.) extracts obtained using various techniques. J Agric Food Chem 2003;51:6604-11.

Peret Almeida L, Cherubino APF, Alves RJ, Dufosse L, Gloria MBA. Separation and determination of Physico-chemical characteristics of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Res Int 2005;38:1039-44.

Pawar HA, Gavasane AJ, Choudhary PD. A novel and simple approach for extraction and isolation of curcuminoid from turmeric rhizomes. Nat Prod Chem Res 2018;6:1-4.

Panigrahi S, Hirlekar R. A new stability-indicating RP-HPLC method for determination of curcumin: an application to nanoparticulate formulation. Int J Pharm Pharm Sci 2016;8:149-55.

Paramasivam M, Poi R, Banerjee H, Bandyopadhyay A. High-performance thin-layer chromatographic method for quantitative determination of curcuminoids in Curcuma longa germplasm. Food Chem 2009;113:640-4.

Chao IC, Wang CM, Li SP, Lin LG, Ye WC, Zhang QW. Simultaneous quantification of three curcuminoid and three volatile components of Curcuma longa using pressurized liquid extraction and high-performance liquid chromatography. Molecules 2018;23:1-9.

Lateh L, Yuenyongsawad S, Chen H, Panichayupakaranant P. A green method for preparation of curcuminoid-rich Curcuma longa extract and evaluation of its anticancer activity. Pharmacogn Mag 2019;15:730-5.

Vadia N, Rajput S. Mesoporous material, MCM-41: a new drug carrier. Asian J Pharm Clin Res 2011;4:44-53.

Bhagiyalakshmi M, Yun LJ, Anuradha R, Jang HT. Utilization of rice husk ash as silica source for the synthesis of mesoporous silicas and their application to CO2 adsorption through TREN/TEPA grafting. J Hazard Mater 2010;175:928-38.

Li T, Shi S, Goel S, Shen X, Xie X, Chen Z, et al. Recent advancements in mesoporous silica nanoparticles towards therapeutic applications for cancer. Acta Biomater 2019;89:1-13.

Sharmiladevi S, Priya AS, Sujitha MV. Synthesis of mesoporous silica nanoparticles and drug loading for gram-positive and gram-negative bacteria. Int J Pharm Pharm Sci 2016;8:196-201.

Sanjay C, Ghate VM, Lewis SA. Mesoporous silica particles for dermal drug delivery: a review. Int J Appl Pharm 2018;10:23-6.

Bolouki A, Rashidi L, Vasheghani Farahani E, Piravi Vanak Z. Study of mesoporous silica nanoparticles as nanocarriers for sustained release of curcumin. Int J Nanosci Nanotechnol 2015;11:139-46.

He Q, Shi J. Mesoporous silica nanoparticle-based nano-drug delivery systems: synthesis, controlled drug release and delivery, pharmacokinetics and biocompatibility. J Mater Chem 2011;21:5845-55.

Patil A, Chirmade UN, Trivedi V, Lamprou DA, Urquhart A, Douroumis D. Encapsulation of water-insoluble drugs in mesoporous silica nanoparticles using supercritical carbon dioxide. J Nanomed Nanotechnol 2011;2:111.

Firmansyah A, Nugrahani I, Wirasutisna KR, Ibrahim S. Formation of boron-silica based mesoporous and studies of its adsorption ability for curcuminoids. Biointerface Res Appl Chem 2020;10:7977-81.

Günaydin Ş, Yilmaz A. Improvement of solubility of celecoxib by inclusion in MCM-41 mesoporous silica: drug loading and release. Turk J Chem 2015;39:317-33.

Trong On D, Joshi PN, Kaliaguine S. Synthesis, stability and state of boron in boron-substituted MCM-41 mesoporous molecular sieves. J Phys Chem 1996;100:6743-8.

Paris JL, Cabañas MV, Manzano M, Vallet Regi M. Polymer-grafted mesoporous silica nanoparticles as ultrasound-responsive drug carriers. ACS Nano 2015;9:11023-33.

Uysal B. Activity of B(OEt)3-MCM-41 catalyst in the MPV reduction of crotonaldehyde. J Chem Sci 2013;125:1385-93.

Du PD, Hieu NT, To TC, Bach LG, Tinh MX, Mau TX, Khieu DQ. Aminopropyl functionalized MCM-41: synthesis and application for adsorption of Pb(II) and Cd(II). Adv Mater Sci Eng 2019;2019:1-15.

Dandekar DV, Gaikar VG. Microwave-assisted extraction of curcuminoids from Curcuma longa. Sep Sci Technol 2002;37:2669-90.

Dandekar DV, Gaikar VG. Hydrotropic extraction of curcuminoids from turmeric. Sep Sci Technol 2003;38:1185-215.



How to Cite




Original Article(s)