FORMULATION OF SERUM GEL CONTAINING ANGELICA KEISKEI LEAF EXTRACT AS AN ANTIOXIDANT AND TYROSINASE ENZYME INHIBITOR

DIAH LIA AULIFA; RIZKA Y. NOERFITRI; DEBY TRISTIYANTI; ARIF BUDIMAN

doi:10.22159/ijap.2020v12i3.37303

Authors

DIAH LIA AULIFA Department of Pharmaceutical Biology, Indonesian School of Pharmacy, JI Soekarno Hatta no.354, Bandung, Indonesia
RIZKA Y. NOERFITRI Department of Pharmaceutical Biology, Indonesian School of Pharmacy, JI Soekarno Hatta no.354, Bandung, Indonesia
DEBY TRISTIYANTI Department of Pharmaceutical Technology, Indonesian School of Pharmacy, JI Soekarno Hatta no.354, Bandung, Indonesia
ARIF BUDIMAN Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy Universitas Padjadjaran, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020v12i3.37303

Keywords:

Angelica keiskei extract, Serum gel, Antioxidant, Tyrosinase enzyme inhibitor

Abstract

Objective: The aim of this study was to formulate and evaluate the efficacy of Angelica keiskei (AK) leaf extract in serum gel as an antioxidant and tyrosinase enzyme inhibitor.

Methods: The leaves of AK were extracted using the maceration method, while the antioxidant and tyrosinase enzyme inhibitory activities were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and L-Tyrosine as assay and substrate, respectively. Subsequently, the extract was formulated into serum gel and evaluated for physical stability, antioxidant and inhibitory activity to tyrosinase enzyme.

Results: The results showed that AK leaf extract has antioxidant activity, with an IC₅₀ value = 7.73 mg/ml, while the tyrosinase enzyme inhibitory power had an IC₅₀ value = 500 mg/ml. Based on physical stability, the serum gel was stable after 28 d of storage. The serum gel of AK leaf extract also showed antioxidant activity and tyrosinase enzyme inhibition with IC₅₀ value of 16.68 mg/ml and 741.62 mg/ml, respectively.

Conclusion: This study demonstrated that the serum gel of AK leaf extract has potential as a skin lightening agent and is safe for use as a topical preparation.

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