DEVELOPMENT AND VALIDATION OF DOUBLE DIVISOR RATIO SPECTRA DERIVATIVE SPECTROPHOTOMETRY METHOD FOR TERNARY MIXTURE OF DEXTROMETHORPHAN HYDROBROMIDE, DOXYLAMINE SUCCINATE AND PSEUDOEPHEDRINE HYDROCHLORIDE IN TABLET DOSAGE FORM

RIDA EVALINA TARIGAN; MUCHLISYAM; SITI MORIN SINAGA; ZUL ALFIAN

doi:10.22159/ijap.2020v12i6.39037

Authors

RIDA EVALINA TARIGAN Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
MUCHLISYAM Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
SITI MORIN SINAGA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
ZUL ALFIAN Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, 20155, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020v12i6.39037

Keywords:

Dextromethorphan hydrobromide, Doxylamine succinate, Pseudoephedrine hydrochloride, Spectrophotometry, Double divisor ratio spectra derivative

Abstract

This study aimed to develop spectrophotometry method by double divisor ratio spectra derivative to determine the levels of dextromethorphan hydrobromide (DEX), doxylamine succinate (DOX) and pseudoephedrine hydrochloride (PSE) in tablet dosage form using ethanol as solvent. The method is based on the use of the coincident spectra of the derivative of the ratio spectra obtained using a double divisor and measuring at the wavelengths selected. Then, the method was applied to determine the levels of DEX, DOX and PSE in tablet dosage form. The selected wavelengths for determination of DEX, DOX and PSE are 277.0 nm, 243.0 nm, and 243.2 nm, respectively. The mean % recoveries were found to be in 100.88%, 100.05%, and 100.26% for DEX, DOX and PSE, respectively. The method is successfully applied to analyze DEX, DOX and PSE in pharmaceutical formulation with no interference from excipients as indicated by the recovery study. All validation parameters were within the acceptable range.

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