• DENI RAHMAT Faculty of Pharmacy, Pancasila University, Jl Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
  • WAHYU WIDOWATI Faculty of Medicine, Maranatha Christian University, Jl Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
  • AHMAD FARIED Department of Neurosurgery and, Oncology and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung 40161, West Java, Indonesia
  • ITA MARGARETHA NAINGGOLAN Faculty of Medicine and Health Sciences, Atma Jaya Catholic University, Jl. Jenderal Sudirman No.51, South Jakarta 12930, Indonesia
  • DIDIK PRIYANDOKO Faculty of Mathematic and Natural Science Education, Indonesia University of Education, Jl. Dr. Setiabudi No. 229, Bandung 40154, West Java, Indonesia
  • ANARISA BUDIATI Faculty of Pharmacy, Pancasila University, Jl Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
  • TEDDY MARCUS ZAKARIA Faculty of Information Technology, Maranatha Christian University, Jl. Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
  • ERVI AFIFAH PT Aretha Medika Utama, Jl. Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia




WJMSC, Microencapsulation, Polymers, Stem cells, Drug delivery


Objective: WJMSC is a stem cell-derived from Wharton's Jelly the umbilical cord of pregnant women which has the ability to differentiate into other cells that are osetogenic, myogenic, neurogenic, and hematopoietic. Stem cell microencapsulation is a cell coating technique that is expected to act as a delivery vehicle. This study aims to make stem cell microencapsulation using various types of natural polymers.

Methods: WJMSC (Wharton's Jelly Mesenchymal Stem Cell) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in incubator with 37 °C and 5% of CO2. In this study, we investigated various of natural polymers (chitosan, glucomannan, inulin, fucoidan and amylopectin) in WJMSC microencapsulation. Viability cell of WJMSC microencapsulate was measured using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, and the condition medium (CM) was determined the measure EGF, IL-6, VEGF with ELISA.

Results: WJMSC microencapsulation using chitosan, oxidized glucomannan, inulin, fucoidan and oxidized amylopectin showed viability cell up to 100%. The EGF, IL-6, VEGF levels were increased in all tested polymers compared to negative control.

Conclusion: Tested polymers (chitosan, oxidized glucomannan, inulin, fucoidan, oxidized amylopectin) were not toxic to WJMSC and cell microencapsulation was successfully carried out.


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How to Cite

RAHMAT, D., WIDOWATI, W., FARIED, A., NAINGGOLAN, I. M., PRIYANDOKO, D., BUDIATI, A., ZAKARIA, T. M., & AFIFAH, E. (2022). MICROENCAPSULATION OF INDONESIAN POLYMER BIODIVERSITY IN WARTHON’S JELLY MESENCHYMAL STEM CELL (WJMSC). International Journal of Applied Pharmaceutics, 14(3), 91–94. https://doi.org/10.22159/ijap.2022.v14s3.19



Original Article(s)