TY - JOUR AU - Sopyan, Iyan AU - Irawati, Widiastuty AU - Indriyati, Wiwiek PY - 2018/01/07 Y2 - 2024/03/28 TI - A NOVEL OF BEZAFIBRATE ANALYSIS METHODS IN URINE (IN VITRO) USING SOLID PHASE EXTRACTION– HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-UV DETECTOR JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 10 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2018v10i1.22769 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/22769 SP - 94-97 AB - <p class="MsoNormal" style="margin-top: 6.0pt; margin-right: 0in; margin-bottom: 6.0pt; margin-left: 0in; text-align: justify; line-height: normal;"><strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN;" lang="EN">Objective: </span></strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN;" lang="EN">Bezafibrate is the second generation of fibrate groups used as the drug of choice in the treatment of hyperlipidemia. The purpose of this study is to obtained a validated method for analyzing bezafibrate in urine using solid phase extraction (SPE)-High performance liquid chromatography (HPLC).</span></p><p class="MsoNormal" style="margin-top: 6.0pt; margin-right: 0in; margin-bottom: 6.0pt; margin-left: 0in; text-align: justify; line-height: normal;"><strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN;" lang="EN">Methods</span></strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN;" lang="EN">:<strong> </strong>Solid phase extraction (SPE) using hydrophilic-lipophilic balance (HLB) cartridge was performed for bezafibrate extraction from urine, afterward, a validation of analysis method using high-performance liquid chromatography (HPLC)-(UV) detection was conducted to parameters, including: selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). </span></p><p class="MsoNormal" style="margin-top: 6.0pt; margin-right: 0in; margin-bottom: 6.0pt; margin-left: 0in; text-align: justify; line-height: normal;"><strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN;" lang="EN">Results</span></strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN;" lang="EN">:<strong> </strong>Recovery extraction using SPE resulted %recovery 85-110%. The analysis was performed by high-performance liquid chromatography using reversed phase, C18 octadecylsilane (ODS) columns 250 x 26 mm, particle size 10 μl, with the composition of 0.01 M acetate buffer with pH 3.55: with percent composition (45:55) and 0.8 ml/minute on 230 nm UV detection. Validation includes selectivity, linearity, accuracy, precision LOD, and LOQ have fulfilled requirement value. </span></p><pre style="margin-top: 6.0pt; margin-right: 0in; margin-bottom: 6.0pt; margin-left: 0in; text-align: justify; tab-stops: .5in;"><strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-ansi-language: EN;" lang="EN">Conclusion</span></strong><span style="font-size: 8.0pt; font-family: 'Cambria','serif'; mso-ansi-language: EN;" lang="EN">:<strong> </strong>The result of recovery extraction using SPE and validation of method exhibited the values that fulfilled the requirements and can be used for analysis bezafibrate in the urine.</span></pre><p><!--[if gte vml 1]><v:shapetype id="_x0000_t32" coordsize="21600,21600" o:spt="32" o:oned="t" path="m,l21600,21600e" filled="f"> <v:path arrowok="t" fillok="f" o:connecttype="none"/> <o:lock v:ext="edit" shapetype="t"/></v:shapetype><v:shape id="_x0000_s1026" type="#_x0000_t32" style='position:absolute; margin-left:-.6pt;margin-top:12.7pt;width:503.05pt;height:.5pt;z-index:251658752' o:connectortype="straight" strokeweight="2.5pt"> <v:shadow color="#868686"/></v:shape><![endif]--><!--[if !vml]--><span style="mso-ignore: vglayout; position: absolute; z-index: 251658752; margin-left: -3px; margin-top: 15px; width: 675px; height: 5px;"><img src="file:///C:/Users/INNOVA~1/AppData/Local/Temp/msohtmlclip1/01/clip_image001.gif" alt="" width="675" height="5" /></span></p> ER -