TY - JOUR AU - IKHSAN, MUHAMMAD AU - HARAHAP, YAHDIANA AU - SAPUTRI, FADLINA CHANY PY - 2022/09/07 Y2 - 2024/03/29 TI - DEVELOPMENT AND VALIDATION OF HPLC-UV METHOD FOR SIMULTANEOUS ANALYSIS OF ACRYLAMIDE AND GLYCIDAMIDE IN VOLUMETRIC ABSORPTIVE MICROSAMPLING JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 14 IS - 5 SE - Original Article(s) DO - 10.22159/ijap.2022v14i5.45440 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/45440 SP - 170-174 AB - <p><strong>Objective: </strong>Acrylamide is a carcinogenic compound that can be found in commonly consumed foods and cigarette smoke. This compound is metabolized by cytochrome P450 in the human body to a more reactive metabolite, glycidamide. This study aimed to optimize and validate a sensitive HPLC-UV method for determining acrylamide and glycidamide simultaneously in the volumetric absorptive microsampling (VAMS) sample.</p><p><strong>Methods: </strong>Isoniazid as an internal standard was added to the VAMS sample containing acrylamide and glycidamide prior to protein precipitation. The analytes and internal standard were separated using reversed-phase chromatography with the C18 Sunfire<sup>TM</sup>Waters® column (5 µm; 250 mm x 4.6 mm) and an ultraviolet detector.</p><p><strong>Results: </strong>The optimum chromatographic condition was eluted at a column temperature of 30 °C with a mobile phase of 6 mmol potassium dihydrogen phosphate pH 3.5–methanol (96:4 v/v) using a flow rate of 0.50 ml/min and was detected at 210 nm. The LLOQ was obtained at 1.0 µg/ml for both acrylamide and glycidamide. The calibration curve was linear over the concentration range of 1.0-100.0 µg/ml.</p><p><strong>Conclusion: </strong>The developed bioanalytical method was valid based on US FDA Guideline for Bioanalytical Method Validation 2018.</p> ER -