FORMULATION AND EVALUATION OF MICROEMULSION CONTAINING NEEM SEED OIL

Abstract

Objective: The objective of the present study was to formulate Microemulsion containing seed oil. Neem seed (Azadirachtaindica) oil was extracted from its seeds by the soxhlet apparatus. Acetone is used as a solvent. PEG 400 and Carbopol 940p was select as surfactant, co-surfactant and hydrogel thickening agent. Microemulsions were characterized for pH, viscosity, spreadability, in vitro drug transport study and in vivo antibacterial activity and shows satisfactory results. Antibacterial activity of formulation against E. coli Shows at a concentration of 3%. The neem seed oil microemulsion has the potential for antibacterial activity.

Methods: A ratio of surfactant and cosurfactant i.e, S/CoSchoosen and corresponding mixture was made. The mixture was mixed with oil. Each mixture was mixed thoroughly using magnetic stirrer until homogenous dispersion/solution was obtained. Double distilled water was used in this formulations as to prevent the incorporation of surface active impurities. The mixture was titrated with water and ambient temperature with constant stirring at the endpoint where the mixture become clouded, the quantity of aquous phase added. The percentage of three different pseudo-phases incorporated were calculated. 

Results: Solubility studies in various solvents reveals that the oil is insoluble in distilled water and ethanol. Soluble in methanol.

Conclusion: It was observed that the microemulsion having multilamellar nature. Batches with carbopol shows better homogenous distribution. The stability of microemulsion prepared with carbopol 71 was gretter than with xanthan gum. The in vitro study of microemulsion was performed and Batch (F7) is optimized batch which shows highest drug release.