EVALUATION OF ANTI-INFLAMMATORY (IN VIVO) ACTIVITY OF ARIFLEX LINIMENT IN COMPARISON WITH DICLOFENAC GEL IN CARRAGEENAN INDUCED RAT PAW EDEMA MODEL
Keywords:Ariflex liniment, Diclofenac gel, Anti-inflammatory activity, Carrageenan induced rat paw edema model
Objective: The present study was conducted to evaluate anti-inflammatory activity of Ariflex liniment (conceptualized and developed by Ari Healthcare Pvt. Ltd) in comparison with Diclofenac gel in carrageenan induced rat paw edema model.
Methods: Wistar rats of either sex weighing 150-180 g were taken and divided into 3 groups with 6 animals in each group i.e. Group 1 (Controlled Group), Group 2 (Diclofenac gel) and Group 3 (Ariflex liniment). The study drugs were topically applied 30 min prior to carrageenan injection. After 30 min 1% w/v of 0.05 ml carrageenan was injected subcutaneously in the paw. The paw was marked with ink at the level of lateral malleolus and immersed in mercury up to the lateral malleolus mark. The paw volume was measured plethysmographically, immediately after injection i.e. on 0 min, and then on 30 min,1h, 2h,3h, 4h and 5hr after injection.
Results: Diclofenac gel sodium treated group showed significant inhibition (p<0.01) of paw edema at 30 min, 1, 2, 3, 4 and 5th hrs as compared to control group. Ariflex Liniment showed significant inhibition (p<0.05) of paw edema at 30 min, 1, 2, 3, and 4th hrs as compared to the control group. Group treated with Ariflex Liniment did not show any significant decrease in paw edema volume at 5th hrs when compared to the control group.
Conclusion: Ariflex Liniment possesses anti-inflammatory activity.
2. Nam JL, Wunthro KL, Vollenhoven RF Van, Pavelka K, Valesini G, Hensor EMA, et al. Current evidence for the management of rheumatoid arthritis with biological disease-modifying antirheumatic drugs: a systematic literature review informing the EULAR recommendations for the management of RA. Ann Rheum Dis 2010;69:976-86.
3. Radha MS, Gangadhar MR. Prevalence of knee osteoarthritis patients in Mysore city, Karnataka. Int J Recent Sci Res 2015;6:3316-20.
4. Mahajan A, Verma S, Tandon V. Osteoarthritis. JAPI 2005;53:634-41.
5. Tallheden T, Bengtsson C, Brantsing C, Carlsson L, Peterson L, Brittberg M, et al. Proliferation and differentiation potential of chondrocytes from osteoarthritic patients. Arthritis Res Ther 2005;7:R560-8.
6. Scheinfeld N. A comprehensive review and evaluation of the side effects of the tumor necrosis factor alpha blockers etanercept, infliximab and adalimumab. J Dermatol Treatment 2004;15:280-94.
7. Kongtharvonskul J, Anothaisintawee T, McEvoy M, Attia J, Woratanarat P, Thakkinstian A. Efficacy and safety of glucosamine, diacerein, and NSAIDs in osteoarthritis knee: a systematic review and network meta-analysis. Eur J Med Res 2015;20:24.
8. Tascioglu F, Armagan O, Tabak Y, Corapci I, Oner C. Low power laser treatment in patients with knee osteoarthritis. Swiss Med Wkly 2004;134:254-8.
9. Shastri B. Yogaratnakar with vidyotini commentary commentary. Shastri L, Poorvardha. Varanasi, Choukhamba Prakashan; 2012. p. 531.
10. Liu W, Qiao W, Liu Z, Wang X, Jiang R, Li S, et al. Gaultheria: phytochemical and pharmacological characteristics. Molecules 2013;18:12071-108.
11. Silva J, Abebe W, Sousa S, Duarte V, Machado M, Matos F. Analgesic and anti-inflammatory effects of essential oils of eucalyptus. J Ethnopharmacol 2003;89:277-83.
12. Hernandez Ortega M, Ortiz Moreno A, Hernandez Navarro M, Chamorro Cevallos G, Dorantes Alvarez L, Necoechea Mondragon H. Antioxidant, antinociceptive, and anti-inflammatory effects of carotenoids extracted from dried pepper (Capsicum Annuum L.). J Biomed Biotechnol 2012. DOI:10.1155/2012/524019
13. Rita de Cassia da Silveira e Sa. A review on anti-inflammatory activity of monoterpenes. Molecules 2013;18:1227-54.
14. Silva Filho SE, de Souza Silva Comar FM, LAM Wiirzler LAM, do Pinho RJ, Grespan R, Bersani Amado CA, et al. Effect of camphor on the behavior of leukocytes in vitro and in vivo in acute inflammatory response. Trop J Pharm Res 2014;13:2031-7.
15. Hashempur MH, Homayouni K, Ashraf A, Salehi A, Taghizadeh M, Heydari M. Effect of linum usitatissimum L. (linseed) oil on mild and moderate carpal tunnel syndrome: a randomized, double-blind, placebo-controlled clinical trial. DARU 2014;22:43.
16. Chunekar KC. Bhavaprakasha nighantu of bhavamishra. Chaukhambha Publications; 2010. p. 809.
17. Flores MP, Castro A, Nascimento J. Topical analgesics. Rev Bras Anestesiol 2012;62:244-52.
18. Reddy V, Rao G, Lakshmi G. A review on anti-arthritic activity of some medicinal plants. J Global Trends Pharm Sci 2014;5:2061-73.
19. Balakrishnan A. Therapeutic uses of peppermint–a review. J Pharm Sci Res 2015;7:474-6.