SPECTROPHOTOMETRIC QUANTIFICATION OF GLICLAZIDE IN PHARMACEUTICAL DOSAGE FORM
Keywords:Spectrophotometric, Quantification, Gliclazide, Cresol Red, Bromophenol blue
Objective: A simple and sensitive spectrophotometric method has been proposed for quantification of Gliclazide in pure and dosage form.
Methods: A visible spectrophotometric methods based on the ion-pair formation of drug with the dye Cresol Red (Method A) and Bromophenol Blue (Method B) in methanol medium.
Results: A purple colour complex was formed in Method A while orange colour was obtained by Method B with maximum absorption at 510 nm and 445 nm, respectively. The Beers law was found linear over the concentration range from 30 to 200µg/ml (Method A) and from 70 to 230µg/ml (Method B) with good correlation coefficients 0.9918 and 0.9916 respectively. The molar extinction coefficient were 0.1326 x 104 L/mol/cm (Method A) and 0.1294 x 104L/mol/cm (Method B), LOD 0.2563, 0.4681 and LOQ 0.6394µg/ml and 0.9256 µg/ml.
Conclusion: The proposed methods were statistically evaluated for accuracy, precision and linearity in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed methods can be applied in routine analytical and quality control laboratories for the quantification of Gliclazide. The methods are simple, sensitive, rapid and economical for the estimation of Gliclazide in pure and dosage forms.
Indian Pharmacopoeia. Vol. 2. New Delhi: Government of India, Ministry of Health and Welfare; 2010. p. 1416-17.
Usharani N, Keerthana K, Sarika M. Development and validation of a novel UV-derivative spectroscopic method for estimation of gliclazide tablets. World J Pharm Res. 2015;4(11):1564-72.
Merck. Index. An encyclopaedia of chemical drugs and BioLogicals. 12th. ed. Vol. 9811; 1996.
Ravi Kumar BVV. A RP-HPLC method development and validation for the estimation of gliclazide in bulk and pharmaceutical dosage forms. J Appl Pharm Sci. 2013;3(4):59-62.
Baghel US, Shrivastava B, Kumar D, Tiwari AP. Gradient HPLC method development and validation for simultaneous estimation of rosiglitazone and gliclazide. Asian Pac J Trop Dis. 2012;2:S756-60. doi: 10.1016/S2222-1808(12)60259-X.
Rouini MR, Mohajer A, Tahami MH. A simple and sensitive HPLC method for determination of gliclazide in human serum. J Chromatogr B Anal Technol Biomed Life Sci. 2003;785(2):383-6. doi: 10.1016/s1570-0232(02)00951-0, PMID 12554153.
AbuRuz S, Millership J, McElnay J. The development and validation of liquid chromatography method for the simultaneous determination of metformin and glipizide, gliclazide, glibenclamide or glimperide in plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;817(2):277-86. doi: 10.1016/j.jchromb.2004.12.018, PMID 15686996.
Agrawal YK, Gogoi PJ, Manna K, Bhatt HG, Jain VK. A supercritical fluid chromatography/tandem mass spectrometry method for the simultaneous quantification of metformin and gliclazide in human plasma. Indian J Pharm Sci. 2010;72(1):50-7. doi: 10.4103/0250-474X.62231, PMID 20582190.
Loni AB, Ghante MR, Sawant SD. Simultaneous UV spectrophotometric method for estimation of gliclazide and metformin hydrochloride in tablet dosage form. Pharm Chem. 2012;4(3):854-9.
Moitra K, Murthy PN, Mishra US. Simultaneous spectrophotometric analysis of rosiglitazone maleate and gliclazide in bulk drug and pharmaceutical formulation. Pharm Sin. 2012;3(2):170-6.
Dadhania KP, Nadpara PA, Agrawal YK. Development and validation of a spectrophotometric method for simultaneous estimation of gliclazide and metformin hydrochloride in bulk and tablet dosage form by simultaneous equation. Int J Pharm Sci Res. 2011;2(6):1559-63.
Reichal CR, Rao MG. Devlopment and validation of a spectrophotometric method for simultaneous estimation of gliclazide and sitagliptin phosphate monohydrate in bulk and pharmaceutical dosage form. Int J Pharm Pharm Sci. 2015;7(6):372-6.
Dhabale PN, Seervi CR. Simultaneous UV spectrophotometric method for estimation of gliclazide and metformin hydrochloride in tablet dosage form. Int J Chem Tech Res. 2010;2(2):813-7.
Pinderjit S, Kumar R, Harinder S. Application of UV spectrophotometric method for analysis of gliclazide in pharmaceutical dosage forms. Int J Pharmacy Pharm Sci. 2011;3(4):259-60.
Jamadar SA, Mulye SP, Karekar PS, Pore YV, Burade KB. Development and validation of UV spectrophotometric method for the determination of gliclazide in tablet dosage form. Pharm Chem. 2011;3(4):338-43.
Dhole SM, Khedekar PB, Amnerkar ND. Application of UV spectrophotometric and HPLC method for simultaneous determination of rosiglitazone maleate and gliclazide in combined tablet dosage form. Int J Pharm Chem Res. 2012;1(1):39-48.
El-Enany N. Spectrofluorimetric and spectrophotometric determination of gliclazide in pharmaceuticals by derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. J AOAC Int. 2003;86(2):209-14. doi: 10.1093/jaoac/86.2.209, PMID 12723907.
Revathi R, Saravanan VS, Mohan RP, Ethiraj T, Ganesan V. Spectrophotometric estimation of gliclazide in bulk and pharmaceutical dosage forms. Int Res J Pharm. 2010;1(1):277-81.
El-Enany N. Spectrophotometric determination of gliclazide in pharmaceuticals and biological fluids through ternary complex formation with eosin and palladium (II). Farmaco. 2004;59(1):63-9. doi: 10.1016/j.farmac.2003.08.007, PMID 14751318.
Chafle DM, Awale LS. Development and validation of direct spectrophotometric method for the estimation of glimepiride. J Pharm Res Int. 2021;33(33A):176-86. doi: 10.9734/jpri/2021/v33i30A31629.
How to Cite
Copyright (c) 2022 DILIP M. CHAFLE
This work is licensed under a Creative Commons Attribution 4.0 International License.