IDENTIFICATION OF GENES/ ENZYMES RESPONSIBLE FOR ANTIBIOTIC RESISTANCE IN VIBRIO ALGINOLYTICUS STRAIN PTS13
Keywords:
Vibrio alginolyticus, Antibiotic, Resistance, Bla TEM, Bla CTX-M, Gyra, Bla VIMAbstract
Vibrio alginolyticus is a halophilic Gram-negative pathogenic bacterium. It is considered as one of the most frequent species living freely in water and sediment and can survive in sea water even in famine conditions while maintaining their virulence. Gram-negative bacteria are highly efficient in up-regulating or acquiring genes that code for mechanisms of antibiotic resistance, especially in the presence of antibiotic selection pressure. Furthermore, they have available to them a plethora of resistance mechanisms, often using multiple mechanisms against the same antibiotic or using a single mechanism to affect multiple antibiotics. This study began with the observation of multi-resistant Vibrio alginolyticus strain recovered from the coastal sediment of Tuticorin beach. The aim of this study was to determine the prevalence of some important resistance genes encoding bla TEM, bla CTX-M, gyrA and bla VIM in the test isolate i. e. Vibrio alginolyticus strain PTS13.
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Acar JF, FW Goldstein. Trends in bacterial resistance to fluoroquinolones. Clin Infect Dis 1997;24(1):67-73.
Andersson DI, Levin BR. The biological cost of antibiotic resistance. Curr Opin Microbiol 1999;2(5):489-93.
Anne Vianney, Tal M Lewin, Wayne F, Beyer Jr, Jean Claude Lazzaroni, Raymond Portalier, et al. Webster, Membrane topology and mutational analysis of the tolq protein of escherichia coli required for the uptake of macromolecules and cell envelope integrity. J Bacteriol 1994;176(3):822-9.
Arlet G, Barrett TJ, Butaye P, Cloeckaert A, Mulvey MR, White DG. Salmonella resistance to extended-spectrum cephalosporins: prevalence and epidemiology. Microbes Infect 2006;8:1945–54.
Baharoglu Z, Mazel D. Vibrio cholerae triggers SOS and mutagenesis in response to a wide range of antibiotics: a route towards multiresistance. Antimicrob Agents Chemother 2011;55:2438–41.
Barlow M, Reik RA, Jacobs SD, Medina M, Meyer MP. High rate of mobilization for blaCTX-M. Emerg Infect 2008;14:423–8.
Bartowsky E, Normark S. Interactions of wild-type and mutant AmpR of Citrobacter freundiiwith target DNA. Mol Microbiol 1993;10:555–65.
Bauer AW, WM Kirby, JC Sherris, M Jurck. Antibiotic susceptibility testing by a standard single disc method. Am J Clin Pathol 1996;451:493-6.
Bauernfeind A, H Grimm, S Schweighart. A new plasmidic cefotaximase in a clinical isolate of Escherichia coli. Infec 1990;18:743-7.
Ben Kahla-Nakbia A, A Besbesa, K Chaieba, M Rouabhiab, A Bakhroufa. Survival of Vibrio alginolyticus in sea water and retention of virulence of its starved cells. Mar Environ Res 2007;46:469-78.
Biedenbach DJ, RN Jones. Fluoroquinolone-resistant Haemophilus influenzae: frequency of occurrence and analysis of confirmed strains in the sentry antimicrobial surveillance program (North and Latin America). Diagn Microbiol Infect 2000;36:255–9.
Biedenbach DJ, GJ Moet, RN Jones. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the sentry antimicrobial surveillance program (1997-2002). Diagn Microbiol Infect 2004;50(1):59-69.
Blazquez J, Couce A, Rodriguez-Beltran J, Rodriguez-Rojas A. Antimicrobials as promoters of genetic variation. Curr Opin Microbiol 2012;15:561–9.
Bonnet R. Growing group of extended-spectrum beta-lactamases: The CTX-M enzymes. Antimicrob Agents Chemother 2004;48:1–14.
Bradford PA. Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 2001;14(4):933-51.
Buschmann AH, Tomova A, López A, Maldonado MA, HenrÃquez LA. Salmon aquaculture and antimicrobial resistance in the marine environment. PLoS One 2012;7(8):1.
Bush K. Bench-to-bedside review: the role of β-lactamases in antibiotic-resistant Gram-negative infections. Crit Care 2010;14(3):224.
Bush K, GA Jacoby. Updated functional classification of β-lactamases. Antimicrob Agents Chemother 2010;54(3):969-76.
Cambau E, F Bordon, E Collatz, L Gutmann. Novel gyrA point mutation in a strain of Escherichia coli resistant to fluoroquinolones but not to nalidixic acid. Antimicrob Agents Chemother 1993;37:1247–52.
Canton R, A Novais, A Valverde, E Machado, L Peixe, F Baquero, et al. Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe. Clin Microbiol Infect 2008;14:144-53.
Canton R, A Oliver, TM Coque, MC Varela, JC Perez-Diaz, Baquero F. Epidemiology of extended-spectrum beta-lactamase-producing Enterobacter isolates in a spanish hospital during a 12-year period. J Clin Microbiol 2002;40:1237–43.
Canton R, TM Coque. The CTX-M beta-lactamases pandemic. Curr Opin Microbiol 2006;9:466-75.
Chanawong A, FH M’Zali, J Heritage, JH Xiong, Hawkey PM. Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among enterobacteriaceae in the people’s republic of china. Antimicrob Agents Chemother 2002;46:630–7.
Christopher T Walsh, John W Trauger, Patrice Courvalin, E Julian, Davies. Mechanism of antibiotic action and resistance. Lancet Infec Diseases; 2001.
Conceicâo TA, Brizio A. Duarte first Isolation of blaVIM-2 in Klebsiella oxytoca clinical Isolates from portugal. Antimicrob Agents Chemother . 2005;49(1):476.
Cornaglia G, Akova M, Amicosante G. Metallo-beta-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues. Int J Antimicrob Agents 2007;29(4):380-8.
Datta N, P Kontomichalou. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature 1965;208:239–41.
Drlica K, X Zao. Microbiol DNA gyrase, topoisomerase IV, and the 4-quinolones. Mol Biol Rev 1997;61:377–92.
Drlica K, H Hiasa, R Kerns, M Malik, A Mustaev, X Zhao. Quinolones: action and resistance updated. Curr Top Med Chem 2009;9:981–98.
Ellington MJ, J Kistler; DM Livermore, Woodford N. Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. J Antimicrob Chemother 2007;59(2):321–2.
Fabrega A, J Sanchez-Cespedes, S Soto, J Vila. Quinolone resistance in the food chain. Int J Antimicrob Agents 2008;31:307–15.
Fritsche TR, HS Sader, MA Toleman. Emerging metallo-beta-lactamase-mediated resistances: a summary report from the worldwide sentry antimicrobial surveillance program. Clin Infect Dis 2005;(41 Suppl 4):S276–8.
Gales AC, KA Gordon, WW Wilke, MA Pfaller, RN Jones. Occurrence of single-point gyrA mutations among ciprofloxacin-susceptible Escherichia coli isolates causing urinary tract infections in latin America. Diagn Microbiol Infect Dis 2000;36:61–4.
Giakkoupi P, L Tzouvelekis, G Daikos, V Miriagou, G Petrikkos, N Legakis, et al. Discrepancies and Interpretation problems in susceptibility testing of VIM-1-producing klebsiella pneumoniae isolates. J Clin Microbiol 2005;43(1):494-6.
Goering RV. Mims' Medical Microbiology 4th ed. Mosby Elsevier; 2008.
Gullberg E, S Cao, OG Berg, C Ilback, L Sandegren, D Hughes, et al. Selection of resistant bacteria at very low antibiotic concentrations. PLoS Pathog. 2011;7:1002-158.
Gutierrez A, L Laureti, S Crussard, H Abida, A Rodriguez Rojas, J Blazquez, et al. J Antimicrob Chemother 2011;66:539–42.
Haldorsen BC, Ø Samuelsen. Multiresistente Gram-negative bakterier-et økende globalt helseproblem. Bioingeniøren. 2012;2:16-21.
Hall BG, M Barlow. Revised ambler classification of β-lactamases. J Antimicrob Chemother 2005;55(6):1050-1.
Harriague A, Covazzi Brino MD, Zampini M, Albertelli G, Pruzzo C, Misic C. "Vibrios in association with sedimentary crustaceans in three beaches of the northern Adriatic Sea (Italy) ". Mar Pollut Bull 2008;56:574-9.
Hawkey PM. Prevalence and clonality of extended-spectrum beta-lactamases in Asia. Clin Microbiol Infect 2008;14:159-65.
Heisig P, H Schedletzky, H Falkenstein-Paul. Mutations in gyrA gene of a highly fluoroquinolone-resistant clinical isolate of Escherichia coli. Antimicrob Agents Chemother 1993;37:696–701.
Herna´ndez-Lucas I, Gallego-Herna´ndez AL, Encarnacio´n S, Ferna´ndez-Mora, M, Martı´nez-Batallar AG, Salgado H, et al. The LysR-type transcriptional regulator LeuO controls expression of several genes in Salmonella enterica serovar Typhi. J Bacteriol 2008;190:1658–70.
Heroven AK, Dersch P. RovM, a novel LysR-type regulator of the virulence activator generovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis. Mol Microbiol 2006;62:1469–83.
Hiroshi Nikaido. Multidrug resistance in bacteria. Annu Rev Biochem 2009;78:119–46.
Jacoby GA. AmpC β-lactamases. Clin Microbiol Rev 2009;22(1):161-82.
Jalal KCA, B Akbar John, BY Kamaruzzaman, K Kathiresan. Antibiotic Resistant Bacteria from Coastal Environment-A Review. In: Marina Pana (ed.) Antibiotic Resistant Bacteria-A Continuous Challenge in the New Millennium. Rijeka, In Tech; 2012. p. 143-58.
Jones RN, DJ Biedenbach, HS Sader. Emerging epidemic of metallo-beta-lactamase-mediated resistances. Diagn Microbiol Infect Dis 2005;51:77–84.
Khosravi Y, ST Tay, J Vadivelu. Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia. J Med Microbiol 2011;60(7):988–94.
Kliebe C, BA Nies, JF Meyer, RM Tolxdorff-Neutzling, B Wiedemann. Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother 1985;288:302–7.
Kohanski MA, MA De Pristo, JJ Collins. Sublethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis. Mol Cell 2010;37:311–20.
Koskiniemi S, M Pranting, E Gullberg, J Nasvall, DI Andersson. Activation of cryptic aminoglycoside resistance in Salmonella enterica. Mol Microbiol 2011;80:1464–78.
Kümmerer K. Antibiotics in the aquatic environment-A review-Part I. Chemosphere 2009;75:417-34.
Kureishi A, JM Diver, B Beckthold, T Schollaardt, LE Bryan. Cloning and nucleotide sequence of Pseudomonas aeruginosa DNA gyrase gyrA-gene from strain PAO1 and quinolone-resistant clinical isolates. Antimicrob Agents Chemother 1994;38:1944–52.
Lazzaroni JC, R Portalier. The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein. Mol Microbiol 1992;6:735-42.
Lin XM, H Li, C Wang, XX Peng. Proteomic analysis of nalidixic acid resistance in Escherichia coli: identification and functional characterization of OM proteins. J Proteome Res 2008;7(6):2399–405.
Livermore DM. β-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 1995;8(4):557-84.
Livermore DM. Bacterial resistance: origins, epidemiology, and impact. Clin Infect Dis 2003;(36 Suppl 1):S11-23.
Madigan MT, JM Martinko. Brock Biology of Microorganisms. Eleventh ed. Pearson Prentice Hall; 2006.
Malik M, KR Marks, A Mustaev, XL Zhao, K Chavda, RJ Kerns, et al. Current Prospects for the Fluoroquinolones as First-Line Tuberculosis Therapy. Antimicrob Agents Chemother 2011;55:2335–43.
Matthew M, Hedges RW, Smith JT. Types of beta-lactamase determined by plasmids in Gram-negative bacteria. J Bacteriol 1979;138:657–62.
Miriagou V, Tzelepi E, Gianneli D, Tzouvelekis LS. Escherichia coli with a self-transferable, multiresistant plasmid coding for metallo-β-lactamase VIM-1. Antimicrob Agents Chemother 2003;47:395–7.
National Nosocomial Infection Surveillance (NNIS) System Report. Data summary from January 1992 through June 2004, issued October 2004. Am J Infect Control 2004;32:470-85.
Nikaido H, M Vaara. Molecular basis of bacterial outer membrane permeability. Microbiol Rev 1985;49:1-32.
Pan XS, KA Gould, LM Fisher. Probing the differential interactions of quinazolinedione PD 0305970 and quinolones with gyrase and topoisomerase IV. Antimicrob Agents Chemother 2009;53:3822–31.
Paterson DL, Ko WC, A Von Gottberg, Mohapatra S, JM Casellas, H Goossens, et al. Implications of extended-spectrum beta-lactamase production in nosocomial infections international prospective study of Klebsiella pneumonia bacteremia. Ann Intern Med 2004;140:26-32.
Paterson DL, KM Hujer, AM Hujer, B Yeiser, MD Bonomo, LB Rice, et al. Extended-spectrum lactamases in Klebsiella pneumonia bloodstream isolates from seven countries: dominance and widespread prevalence of SHV-and CTX-M-type-lactamases. Antimicrob Agents Chemother 2003;47:3554–60.
Peleg A, Franklin C, Bell J, Spelmam D. Dissemination of the metallo β-lactamase gene bla IMP-4 among gram-negative pathogens in a clinical setting in Australia Clin Infect Dis 2005;41:1549-56.
Peleg AY, DC Hooper. Hospital-acquired infections due to Gram-negative bacteria. N Engl J Med 2010;362:1804-13.
Perez F, A Endimiani, KM Hujer, RA Bonomo. The continuing challenge of ESBLs.†Curr Opin Pharmacol 2007;7(5):459–69.
Pitout JD, Hossain A, Hanson ND. Phenotypic and molecular detection of CTX-M-beta-lactamases produced by Escherichia coli and Klebsiella spp. J Clin Microbiol 2004;42:5715–21.
Pitout JD, KB Laupland. Extended-spectrum-beta-lactamse-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis 2008;8:159-66.
Pitout JD, KB Laupland, L Poirel. Emergence of Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBLs n the community. J Antimicrob Chemother 2005;56:52-9.
Pitout JDD, BL Chow, Gregson DB, KB Laupland, S Elsayed, DL Church. Molecular epidemiology of metallo-β-lactamase-producing Pseudomonas aeruginosa in the Calgary Health Region: emergence of VIM-2-producing isolates. J Clin Microbiol 2007;45(2):294–8.
Podbielski A, J Schonling, B Melzer, K Warnatz, H-G Leusch. Molecular characterization of a new plasmid-encoded SHV-type beta-lactamase (SHV-2 variant) conferring high-level cefotaxime resistance upon Klebsiella pneumoniae. J Gen Microbiol 1991;137:569–78.
Poole K. Resistance to β-lactam antibiotics. Cell Mol Life Sci 2004;61:2200–23.
Quale JM, D Landman, PA Bradford, M Visalli, J Ravishankar, Flores, et al. Molecular epidemiology of a citywide outbreak of extended-spectrum β-lactamase producing Klebsiella pneumoniae infections. Clin Infect Dis 2002;35:834-41.
Raskin C, Ge´rard C, Donfut S, Giannotta E, Van Driessche G, Van Beeumen J, et al. BlaB, a protein involved in the regulation of Streptomyces cacaoi β-lactamases, is a penicillin-binding protein. Cell Mol Life Sci 2003;60:1460–9.
Rheinheimer G. Microbial ecology of brakish water environment. Ecol Stud 1977;28:39-60.
Robicsek A. Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. Nat Med 2006;12(1):83-8.
Rossolini GM, MM D’Andrea, C Mugnaioli. The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect 2008;14:33-41.
Ruiz J. Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection. J Antimicrob Chemother 2003;51(5):1109-17.
Sader H, M Castanheira, R Mendes, M Toleman, T Walsh, R Jones. Dissemination and diversity of metalo b-lactamases in Latin America: report from the sentry antimicrobial surveillance program. Int J Antimic Agents 2005;25:57-61.
Saiki RK, DH Gelfand, S Stoffel, SJ Scharf, R Higuchi, GT Horn, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Sci 1988;239:487-91.
Sambrook J, DW Ressell. Molecular cloning-A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York: 3rd edition; 2001.
Sánchez D, D Marcano, E Spadola, L León, D Payares, C Ugarte, et al. Metaloenzimas tipo VIM detectadas en aislamientos clÃnicos en pseudomonas aeruginosa en cuatro hospitales en venezuela. Revista del Instituto Nacional de Higiene Rafael Rangel 2008;39(2):17-22.
Sarah E Maddocks, CF Petra. Structure and function of the LysR-type transcriptional regulator (LTTR) family protein. Rev Oyston Microbiol 2008;154:3609–23.
Schell MA. Molecular biology of the LysR family of transcriptional regulators. Annu Rev Microbiol 1993;47:597–626.
Sirot D, J Sirot, R Labia. Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel beta-lactamase. J Antimicrob Chemother 1987;20:323–34.
Sougakoff W, S Goussard, P Courvalin. The TEM-3 beta-lactamase, which hydrolyzes broad-spectrum cephalosporins, is derived from the TEM-2 penicillinase by two amino acid substitutions. FEMS Microbiol Lett 1988;56:343–8.
Stec E, Witkowska-Zimny M, Hryniewicz MM, Neumann P, Wilkinson AJ, Brzozowski AM, et al. Structural basis of the sulphate starvationresponse in E. coli: crystal structure and mutational analysis of the cofactor-binding domain of the Cbl transcriptional regulator. J Mol Biol 2006;364:309–22.
Sun J, Klein A. A LysR-type regulator is involved in the negative regulation of genes encoding selenium-free hydrogenases in the archaeon Methanococcus voltae. Mol Microbiol 2004;52:563–71.
Thi TD, E Lopez, A Rodriguez-Rojas, J Rodriguez-Beltran, A Couce, J R Guelfo, et al. Effect of recA inactivation on mutagenesis of Escherichia coli exposed to sublethal concentrations of antimicrobials. J Antimicrob Chemother 2011;66:531–8.
Toleman M, Biedenbach D, Bennett D, Jones R, Walsh T. Italian metallo-lactamases: a national problem? Report from the sentry antimicrobial surveillance programme. J Antimicrob Chemother 2005;55:61-70.
Toleman MA, Simm AM, Murphy TA, Gales AC, Biedenbach DJ, Jones RN, et al. Molecular characterization of SPM-1, a novel metallo-b-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme. J Antimicrob Chemother 2002;50:673–9.
Tzouvelekis LS, E Tzelepi, PT Tassios, NJ Legakis. CTX-M-type β-lactamases: an emerging group of extended-spectrum enzymes. Int J Antimicrob Agents 2000;14:137-42.
Vila J, J Ruiz, F Marco, A Barcelo, P Gon˜i, E Giralt, et al. Association between double mutation in gyrA gene of ciprofloxacin-resistant clinical isolates of Escherichia coli and MICs. Antimicrob Agents Chemother 1994;38:2477–9.
Vila J, J Ruiz, P Goni, A Marcos, T Jimenez de Anta. Mutation in the gyrA gene of quinolone-resistant clinical isolates of Acinetobacter baumannii. Antimicrob Agents Chemother 1995;39:1201–03.
Villegas MV, JN Kattan, MG Quinoteros, JM Casellas. Prevalence of extended-spectrum beta-lactamases in South America. Clin Microbiol Infect 2008;14:154-8.
Walsch TR, MA Toleman, L Poirel, P Nordmann. Metallo-β-Lactamases: the Quiet before the Storm? J Clin Microbiol 2002;18:3798–801.
Walsch TR, MA Toleman, L Poirel, P Nordmann. Metallo-beta-lactamases: the quiet before the storm? Clin Microbiol Rev 2005;18:306-25.
Wang Y, WM Huang, DE Taylor. Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations. Antimicrob Agents Chemother 1993;37:457–63.
Weisburg WG, SM Barns, DA Pelletier, DJ Lane. 16S ribosomal DNA amplification for phylogenetic study". J Bacteriol 1991;173(2):697–703.
Yang-Ree Kim, Sang-Il Kim, Ji-Young Lee, Y eon-Joon Park, Kyo-Young Lee, Moon-Won Kang. Nosocomial Transmission of CTX-M-15 and OXA-30 β-Lactamase-producing escherichia coli in a neurosurgical intensive care unit. Ann Clin Lab Sci 2005;35:3.