ANTIOXIDANT, DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITIES OF AMORPHOPHALLUS CAMPANULATUS
Objective: Amorphophallus campanulatus (AC) is commonly known as Suran. It is used to cure piles, deranged digestion and liver diseases. The present study was carried out to evaluate AC plant as a new source of natural bioactive molecules having antioxidant potential and their protective role against hepatotoxicity induced by t-BHP.
Methods: Ethanolic extracts of AC leaf and fruit were subjected to assessment of its antioxidant potential by superoxide anion radical scavenging activity (SARSA), free radical scavenging activity (FRSA), ferrous ion chelation activity (FIC) and reducing power (RP). The study was further carried out to assay the DNA damage and hepatoprotective activity of AC leaf extract.
Results: Leaf extract of AC showed lower IC50 for SARSA, FRSA and FIC (111.67 mg/ml, 11.58 mg/ml and 8.56 mg/ml, respectively) than fruit extract. HPLC analysis of leaf showed the presence of chlorogenic acid, caffeic acid, rutin, quercetin and kaempherol. Leaf extract showed potential to protect hydroxyl radical-induced DNA damage. Hepatoprotective effects of AC leaf was shown by increasing superoxide dismutase (SOD), glutathione content (GSH), catalase (CAT) and protein content and decreasing nitric oxide (NO) and MDA formation, when t-BHP treated hepatocyte cells were exposed to different concentration (1.0, 2.5, 5.0 and 7.5 Âµg) of extract.
Conclusion: The results suggest that AC leaf has antioxidant, cytoprotective and hepatoprotective properties which may be due to the presence of important bioactive constituents; hence it may be used in the form of dietary component and also in formulations against liver diseases.
Keywords: Hepatoprotective activity, Glutathione, Antioxidant enzyme, Lipid Peroxidation, Hepatocytes
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