• Shilpam Sinha
  • Richa Raghuwanshi Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India


Objective: In this study, phytochemical screening, antioxidant activity, total phenolic, flavonoid contents and reducing potential of Eclipta prostrata (L.) L plant prepared in different solvents (methanolic, ethanolic and double distilled water) was evaluated by various assays.

Methods: Polyphenolic contents, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, Hydrogen peroxide (H2O2) radical scavenging activity and reducing power of the plant was estimated by modified protocol.

Results: In DPPH free radical, methanolic extract of plant was most potent in activity with 50% inhibition at 333.34 µg/ml. and 398.12 µg/ml concentration respectively. Total phenolic content was maximum in the methanolic extract of plant (52.5±0.64 mg/g of gallic acid equivalent) and total flavonoid was maximum in aqueous extract of plant (193.12±1.65 mg/g of rutin equivalent). High reducing capacity of plant was observed in case of methanolic extract. A significant correlation was found between antioxidant activity and polyphenolic content (total phenols and total flavonoids). Moreover, a significant correlation was found between antioxidant activity and reducing potential of plant extract, denoting that reducers are important contributors to antioxidant.

Conclusion: The study shows E. prostrata as an important natural source of antioxidants.

Keywords: Eclipta prostrata, Plant extract, Antioxidant activity, Polyphenolic content, DPPH activity, H2O2 scavenging activity, Reducing potential


Download data is not yet available.


Manian R, Anusuya N, Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem 2008; 107:1000–7.

Muramatsu H, Kogawa K, Tanaka M. Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility. Cancer Res 1995;55:6210–4.

Ames BN, Shigenaga M, Hagera T. Oxidants, antioxidants and degenerative diseases of aging. Proc Natl Acad Sci 1993;90:7915-22.

Larson RA. The antioxidants of higher plants. Phytochemistry 1988;27:969-78.

Williamson G, Rhodes MJ, dan Parr AJ. Disease prevention and plant dietary substances. Chemical from prospectives on plant secondary product. Imperial College Press: World Science; 1999.

Halliwell B, Gutteridge JC. The definition and measurement of antioxidants in biological systems. Free Radical Biol Med 1995;18:125–6.

Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation heart disease and cancer. Pharmacol Rev 2000;52:673–751.

Carocho M, Barreiro MF, Morales P, Ferreira ICFR. Adding molecules to food, pros and cons: a review of synthetic and natural food additives. Compr Rev Food Sci Food Saf 2014; 13:377-99.

Moure A, Cruz JM, Franco D, Domínguez JM, Sineiro J, Domínguez H, et al. Natural antioxidants from residual sources. Food Chem 2001;72:145-71.

Zheng W, Wang SY. Antioxidant activity and phenolic compound in selected herbs. J Agric Food Chem 2001;49:5165-70.

Wresburger JH. Lifestyle, health and disease prevention: The underlying mechanism. Eur J Cancer Prev 2002;S2:1-7.

Sultana B, Anwar P. Flavonols (kaempeferol, quercetin, myricetin) contents of selected fruits, vegetables and medicinal plants. Food Chem 2008;108:879-84.

Eloff JN. It is possible to use herbarium specimens to screen for antibacterial components in some plants. J Ethnopharmacol 1999;67:355-60.

Datta K, Singh AT, Mukherjee A, Bhat B, Ramesh B, Burman AC. Ecliptaalba extracts with potential for hair growth promoting activity. J Ethnopharmacol 2009;124:450–6.

Ma-Ma K, Nyunt N, Tin KM. The protective effect of Eclipta alba on carbon tetrachloride-induced acute liver damage. Toxicol Appl Pharm 1978;45:723–8.

Jaiswal N, Bhatia V, Srivastava SP, Srivastava AK, Tamrakar AK. Antidiabetic effect of Eclipta alba associated with the inhibition of ð›¼-glucosidase and aldose reductase. Nat Prod Res 2012;26:2363–7.

Panghal M, Kaushal V, Yadav JP. In vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases. Ann Clin Microbiol Antimicrob 2011;10:21.

Chaudhary H, Dhuna V, Singh J, Kamboj SS, Seshadri S. Evaluation of a hydro-alcoholic extract of Eclipta alba for its anticancer potential: an in vitro study. J Ethnopharmacol 2011;136:363–7.

Singh R, Kumari N, Gangwar M, Nath G. Qualitative characterization of phytochemicals and in vitro antimicrobial evaluation of leaf extract of Couroupita Guianensis Aubl.-a threatened medicinl tree. Int J Pharm Pharm Sci 2015;7:212-5.

Harborne JB. A guide to modern techniques of plant analysis; phytochemical methods, London. Chapman and Hall, Ltd; 1973. p. 49-188.

Sofowora A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd. (Pub.), Ibandan, Nigeria; 1993. p. 289.

McCune LM, Johns T. Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest. J Ethnopharmacol 2002;82:197–205.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003–8.

McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.

Athukorala Y, Jeon Y, Kim K. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol 2006;44:1065-74.

Hossain MS, Alam MB, Chowdhury NS, Asadujjaman M, Zahan R, Islam MM, et al. Antioxidant, analgesic and anti-inflammatory activities of the herb E. prostrata. J Pharm Toxicol 2011;6:468-80.

Santhosh S, Velmurugan S, Annadurai R. Phytochemical screening and antimicrobial activity of medicinal plants (Eclipta prostrata L. and Sphaeranthusindicus L.). Int J Pure Applied Biosci 2015;3:271-9.

Huang WY, Cai YZ, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer 2010;62:1-20.

Wolfe KL, Kang X, He X, Dong M, Zhang Q, Liu RH. Cellular antioxidant activity of common fruits. J Agric Food Chem 2008;56:8418-26.

Koleva II, Van Breek TA, Linssen JPH, De Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002;13:8-17.

Chen CW, Ho CT. Antioxidant properties of polyphenols extracted from green and black teas. J Food Lipids 1995;2:35-46.

Al-Owaisi M, Al-Hadiwi N, Khan SA. GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrine (Forssk.) Fiori leaves. Asian Pac J Trop Biomed 2014;4:964-70.

Gurusamy K, Saranya P. In vitro antioxidant potential of ethanolic contents of Eclipta alba and Wedelia chinensis. J Pharm Res 2010;3:2825-7.

Keser S, Celik S, Turkoglu S, Yilmaz O, Turkoglu I. Hydrogen peroxide radical scavenging and total antioxidant activity of Hawthorn. Chem J 2012;2:9-12.

Mishra SL, Sinhamahapatra PK, Nayak A, Das R, Sannigrahi S. In vitro antioxidant potential of different parts of Oxoxylum indicum: a comparative study. Indian J Pharm Sci 2010;72:267-9.

Perez-Jimenez J, Arranz S, Tabernero M, Diaz-Rubio ME, Serrano J, Goni I. Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction, measurement and expression of results. Food Res Int 2008;41:274–85.

Zaman RU, Ghaffar M, Fayyaz T, Mehdi S. In vitro evaluation of total phenolics and antioxidant activities of Withania somnifera, Eclipta prostrata L., Gossypium herbasceum L. J Appl Pharm 2011;1:133-44.

Liu X, Dong M, Chen X, Jiang M, Lv X, Yan G. Antioxidant activity and phenolics of an endophytic Xylaria sp. from Gingko biloba. J Food Chem 2007;105:548-54.

Sulaiman SF, Sajak AAB, Supriatno KLO, Seow EM. Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables. J Food Compos Anal 2011;24:506–15.

Das, NP, Pereira TA. Effects of Flavonoids on thermal autooxidation of palm oil: structure activity relationship. J Am Chem Soc 1990;67:255-8.

Hsu B, Coupar IM, Ng K. Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. Food Chem 2006;98:317-28.

Ramesh V, Hari R, Pandian S, Arumugam G. Antioxidant activity of combined ethanolic extract of Eclipta alba and Piper longum Linn. J Complementary Integr Med 2011;8:1-13.

Kratchanova M, Deher P, Ciz M, Lozek A, Mihailrv A. Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Comparison of two extraction systems. Acta Biochim Pol 2010;57:229-34.

Singh R, Kumari N. Comparative determination of phytochemicals and antioxidant activity from leaf and antioxidant activity from leaf and fruit of Sapindus mukorrossi Gaertn.-A valuable medicinal tree. Ind Crop Prod 2015;73:1-8.



How to Cite

Sinha, S., and R. Raghuwanshi. “PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF E. PROSTRATA (L.) L- A VALUABLE HERB”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 3, Mar. 2016, pp. 255-60,



Original Article(s)