IN VITRO ANTHELMINTIC ACTIVITY OF AQUEOUS AND METHANOLIC EXTRACTS OF OLDENLANDIA UMBELLATA

Authors

  • Somnath D. E. Dr. Samuel George Institute of Pharmaceutical Sciences, Markapur- 523316, Andhra Pradesh
  • Akalanka Dey Annamalai University, Department of Pharmacy, Annamalai Nagar-600 802, Tamil Nadu, India
  • A. M. S. Sudhakar Babu A.M.Reddy Memorial Colleges of Pharmacy, Narasaraopet, Guntur-522601, Andhra Pradesh.
  • Siddabathuni Aneela Dr. Samuel George Institute of Pharmaceutical Sciences, Markapur- 523316, Andhra Prades

Keywords:

Anthelmintic activity, Pheretima posthuma, Oldenlandia umbelata, Aqueous extract, Methanol extract

Abstract

Objective: The present study was carried out to evaluate the in vitro anthelmintic activity of aqueous and methanolic extracts of Oldenlandia umbelata against Pheretima posthuma.

Methods: Three different concentrations, each of crude alcoholic and aqueous extract (10, 50,100 mg/ml in distilled water) were prepared and six worms (same type) were placed in it. Observations were made for the time taken to cause paralysis and death of the individual worms. Mean time for the paralysis (P) in min was noted when no movement of any sort could be observed,except when the worm was shaken vigorously; time of death (D) in min was recorded after ascertaining the worms neither moved when shaken vigorously nor when dipped in warm water (50°C). Piperazine citrate (10mg/ml) was included as reference compounds.

Results: The extracts of Oldenlandia umbelata not only demonstrated paralysis, but also caused death of worms, especially at higher concentration of 100 mg/ml in shorter time as compared to reference drug Piperazine citrate.

Conclusion: In present statement methanolic and aqueous extracts of plant of Oldenlandia umbelata was investigated for their anthelmintic activity against Pheretima posthuma. Various concentrations were used in the bioassay, which involved paralysis and death time of the worms. Both the extracts showed significant anthelmintic activity.

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References

Thomson WAR. Medicines from the Earth1978.

World D, Series WHO. WHO, Diet, Nutrition and the prevention of Chronic Organization Technical Report Geneva. J Biochemistry 2003;916.

Etkin NL. A Hausa herbal pharmacopoeia:biomedical evaluation of commonly used plant medicines. J Ethnopharmacol 1981;4(1):75-98.

Preethi R, Devanathan VV, Loganathan M. Antimicrobial and Antioxidant Efficacy of Some Medicinal Plants against Food Borne Pathogens. J Adv In bio Res 2010;4(2):122-5.

Csir. J Wealth of India 1959;5.

Kirtikar KR, Basu BD. J Indian Medicinal Plants 1935.

Purushothaman KK, Sarada K, Narayanasami V. Imbural ( Oldenlandia umbellata). J Res Indian Med Biochemistry 1972;7(3).

Gupta M, Mazumder UK, Thamilselvan V, Manikandan L, Senthilkumar GP, Suresh R, et al. Potential hepatoprotective effect and antioxidant role of methanol extract of Oldenlandia umbellata in carbon tetrachloride induced hepatotoxicity in Wistar rats. J Pharmacol Ther 2007;6:5-9.

Nadkarni, K. D. J Indian Materia Medica:Popular Prakashan, Bombay, India, 1979.p. 869.

Kirtikar, K. R and Basu, B. D. J Indian Medicinal Plants Bishen:mahendra pal singh, Dehradun, India, 1975. p. 842-44.

Cardon, D. du Chatenet, G. 1990. Guide des teintures naturelles.Delachaux et NiestleS.A, Paris.

Adejimi JO, Harrison LJS. Parasitic Nematodes of Domestic Ruminants in Nigeria;Impact on Ruminant Production and Control. Trop Vet 15.1997:137-48.

Y. M. Shivkar, V. L. Kumar.;Anthelmintic activity of latex of Calotropis procera. J Pharm Biol 2003;41:263-65.

Mali JC, Hundiwale RS, Sonawane RN. R. G. Patil and B. C Hatapakki Evaluation of Capparis decidua for anthelmintic and antimicrobial activities. Ind J Nat Prod 20.2004:10-3.

Siddabathuni D. Somnath Akalanka Dey, and A. MS Sudhakar Babu GCMS analysis of phytocomponents in the methanolic extract of Oldenlandia umbellata. Int J of Chemical and Pharm Sci Dec 2013;4(4):7-10.

Published

01-07-2014

How to Cite

D. E., S., A. Dey, A. M. S. S. Babu, and S. Aneela. “IN VITRO ANTHELMINTIC ACTIVITY OF AQUEOUS AND METHANOLIC EXTRACTS OF OLDENLANDIA UMBELLATA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 94-95, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1266.

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