PRELIMINARY PHYTOCHEMICAL AND STANDARDIZATION PARAMETERS OF IPOMOEA QUAMOCLIT LINN WHOLE PLANT-AN ETHNOMEDICINALLY IMPORTANT PLANT
Keywords:Ipomoea quamoclit, Phytochemical screening, Proximate values, Fluorescence analysis
Objective: Preliminary phytochemical studies and proximate analysis of Ipomoea quamoclit Linn whole plant.
Methods: The whole plant was collected, shade dried and made into powder. The powdered plant material was studied for its proximate values which include ash values, extractive values, fluorescence analysis and moisture content by standard methods. The powdered plant material was subjected to successive solvent extraction by maceration using petroleum ether, chloroform, ethyl acetate, methanol, water and hydroalcoholic mixture as solvents. All the extracts were subjected to a preliminary phytochemical screening in which chemical tests were carried out for the detection of various phytoconstituents.
Results: Proximate analysis revealed that the dry plant powder has 9.68% total ash, 3.57% acid insoluble ash, 2.95% water soluble ash, 3.49% sulphated ash, 12.92% alcohol soluble extractive value, 9.45% water soluble extractive values, 5.41% ether soluble extractive value and 5.25% moisture content. The whole plant powder found to possess phytoconstituents like alkaloids, carbohydrates, saponins, phenolic compounds, tannins, phytosterols, amino acids, proteins and flavonoids. Fluorescence analysis revealed the behavior of the plant powder when treated with different chemical reagents and observation under UV light at 365 nm.
Conclusion: The present study reveals the preliminary phytochemical and proximate analysis of Ipomoea quamoclit whole plant.
Raskin I, David RM, Komarnytsky S, Nebojsa I. Plants and human health in the twenty-first century. J Trends Biotechnol 2002;20 (12):552-30.
Chan K. Some aspects of toxic contaminants in herbal medicines. Chemosphere 2003;52:1361-71.
Chetty KM, Sivaji K, Rao KT. Flowering plants of Chittoor district, Andhra Pradesh, India. 1st ed. Tirupati (India):Student offset printers;2008.
Pullaiah T, Chennaiah E. Flora of Andhra Pradesh. 1st ed. Vol. 1. Jodhpur (India):Scientific Publishers;1997.
Hasan SMR, Hossain MM, Raushanara A, Mariam J, Mazumder HEM, Rahman S. DPPH free radical scavenging activity of some Bangladeshi medicinal plants. J of Med Plant Res 2009;3 (11):875-9.
Khare CP. Indian medicinal plants. 1st ed. New Delhi (India):Spinger;2007.
Kristina JS, Sonja CO, Thomas S, Karsten S, Frank M, Monika H. Ipangulines and minalobines, chemotaxonomic markers of the infrageneric Ipomoea taxon subgenus Quamoclit, section Mina. J Phytochem 2005;66:223-31.
Mahabir PG, Ludger W, Hartmann T. Pyrrolizidine alkaloids from Ipomoea hederifolia and related species. J Phytochem 1998;47 (8):1551-60.
Diego AP, Harborne JB. New sources of ergoline alkaloids within the genus Ipomoea. J Biochem Sys Ecology 1993;21 (4):455-61.
A Survey of the Anthocyanins of some Tropical Plants. J Proc Royal Society of London 1954;549-64.
Rastogi RP, Mehrotra BN. Compendium of Indian medicinal plants-an illustrated dictionary. 2nd ed. Volume III. Delhi (India):Directorate of information. J Council for Scientific and Industrial Res (CSIR) 2007.
Manjulika Y, Sanjukta C, Sharad KG, Geeta W. Preliminary Phytochemical Screening of Six Medicinal Plants Used In Traditional Medicine. Int J of Pharm and Pharm Sci 2014;6 (5):539-42.
Prasanna K, Yuwvaranni S. Preliminary Phytochemical Screening and Antibacterial activity of Datura metel And Vitex negundo Against Bacterial Cold Water Disease Causing Organism. Int J of Pharm and Pharm Sci 2014;6 (5):230-3.
Fred JA, Adisa A. Phytochemical and Pharmacognostic Studies of Telosma africanum (N.E.Br) Colville Leaf and Stem. Int J of Pharm Sci and Res 2012;3(6):1860-62.
Kokate CK. Practical Pharmacognosy. 3rd ed. New Delhi:Vallabh Prakashan;1994.