ANTIDEPRESSANT AND NOOTROPIC ACTIVITY OF AQUEOUS EXTRACT OF INDIGOFERA TINCTORIA IN MICE
Keywords:
Depression, Nootropic activity, Indigofera tinctoria, AntioxidantAbstract
Objective: The present study was undertaken to evaluate the antidepressant and nootropic potential of aqueous extract of Indigofera tinctoria (ITAE).
Methods: Antidepressant activity of the aqueous extract of Indigofera tinctoria was evaluated in mice by using the forced swim test (FST), tail suspension tests (TST) and tetrabenazine induced catalepsy and ptosis models at doses of 250 and 500 mg/kg orally. Nootropic activity was assessed using elevated plus maze and Morris water maze. Imipramine (25 mg/kg) and Piracetam (300 mg/kg) were used as standard drugs for antidepressant and nootropic activity respectively. Antioxidant assays like DPPH and TBARS was also carried to support the antidepressant and nootropic activity.
Results: Pre-treatment with ITAE showed significant dose dependent reduction in immobility time displayed in both, FST and TST, when compared to that of vehicle.(P<0.05). Also, significant reduction in tetrabenazine induced catalepsy and ptosis was observed. Moreover ITAE showed dose dependent cognitive enhancing activity in Morris water maze and elevated plus-maze. ITAE exhibited IC50 value of 17.39 μg/ ml in DPPH assay and 398. 71 μgm/ ml in TBARS assay.
Conclusion: ITAE exhibits antidepressant and nootropic activities at the dose of 250 and 500 mg/ kg which are comparable to imipramine and piracetam respectively which could be attributed to its effect on neurotransmitters and antioxidant activity respectively.
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Shoeb A, Chowta M, Pallempatti G, Rai A, Singh A. Evaluation of antidepressant activity of vanillin in mice. Indian J of Pharmacology 2013;45:141-4.
Jon Nash, David Nutt. Antidepressants. J Psychiatry 2007;6 (7):289-94.
Diana Byrd Burt, Mary Jo Zembar. Depression and Memory Impairment:A Meta-Analysis of the Association, Its Pattern, and Specificity. J Psychopharmacol Bull 1995;117 (2):285-305.
Ramanathan M, S N Ashok Kumar, B Suresh. Evaluation of cognitive function of fluoxetine, sertaline and tianeptine in isolation and chronic unpredictable stress. Indian J Exp Biol 2003;41:1269-72.
Singh R.H, Mishra L.C. Psychiatric Disorders. In:Scientific Basis for Ayurvedic Therapies. J USA CRC Press 2003:439-51.
Singh A, Duggal S. Piperine-Review of advances in Pharmacology. Int J of Pharm Sci and Nanotechnology 2009;2(3):615-20.
Warrier PK, Nambiar VPK, C Ramankatty. In:Indian Medicinal Plants. Chennai:Orient Longman Private Limited;2007;3:210.
Saraswathi Motamarri N, Karthikeyan M, Rajasekar S, Gopal V. Indigofera tinctoria Linn-A Phytopharmacological Review. Int J of Res in Pharm and Biomedical Sci 2012;3 (1):164-9.
Spandana Rajendra Kopalli, Sushruta Kopalla, Ki Young Shin, Su-Jin Noh, Qinghao Jin, Bang Yeon Hwang et al. SF-6 attenuates 6-hydroxydopamine-induced neurotoxicity:An in vitro and in vivo investigation in experimental models of Parkinson’s disease. J Ethnopharmacol 2012;143:686-94.
Khandelwal. Preliminary Phytochemical Screening. In:Practical Pharmacognosy 3rd ed. Pune:Nirali Prakashan;1996.p.149-55.
Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice:A primary screening test for antidepressants. J Archives Int de Pharmacodynamie et de Therapie 1977;229:327-36.
Steru L, Chermat R, Thierry B, Simon P. The tail suspension test:A new method for screening antidepressants in mice. J Psychopharmacol 1985;85 (3):367-70.
Vogel GH. Psychotropic and Neurotropic activity, In Drug discovery and Evaluation-Pharmacological assays, 2nd ed. Germany:Springer publication;2002.p. 568.
Mrugaya P Kulkarni and Archana R Juvekar. Effects of Alstonia scholaris (Linn.) R.Br on stress and cognition in mice. Indian J Exp Biol 2008;47:47-52.
Annapurna, V. Rama Krishna, K. Murali Krishna, V. Krishna Kumar and K. B. Prasad. Studies on Nootropic activity of Ramipril and Losartan on Scopolamine-induced amnesia in Rats. Indian J Pharm Sci 2004;66(1):31-5.
Ketkee Deshmukh, Purnima Amin. Meltlets of Soy Isoflavones:Process optimization and the Effect of Extrusion Spheronization Process Parameters on Antioxidant activity. Indian J Pharm Sci 2013;75 (4):450-56.
Hiroshi Ohkawa, Nobuko Ohishi, Kunio Yagi. Assay for Lipid Peroxides in Animal Tissues by Thiobarbaturic Acid Reaction. J Anal Biochem 1979;95:351-8.
Guilherme A. Behr, José C.F. Moreira and Benicio N. Frey. Preclinical and clinical evidence of Antioxidant effects of Antidepressant agents:Implications for the pathophysiology of Major Depressive disorder. Oxidative Medicine and Cellular Longevity 2012;1.
Ann Gardner, Richard G. Boles. Beyond the serotonin hypothesis:Mitochondria, inflammation and neurodegeneration in major depression and affective spectrum disorders. J Prog Neuropsychopharmacol Biol Psychiatry 2011;35:730-43.
Jingshu Liu, Wei Qiao, Yi Yang, Liyan Ren, Yan Sun, Shaoxun Wang. Antidepressant-like effect of the ethanolic extract from Suanzaorenhehua formula in mice models of depression. J Ethnopharmacol 2012;141:257–64.
Li-Tao Yi, Jian-Mei Li, Yu-Cheng Li, Ying Pan, Qun Xu, Ling-Dong Kong. Antidepressant-like behavioral and neurochemical effects of the citrus-associated chemical apigenin. J Life Sci 2008;82:741-51.
Warjeet S, Laitonjam and Sujata D. Wangkheirakpam. Comparative study of the major components of the indigo dye obtained from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn. Int J Plant Physiol Biochem 2011;3(7):108-16.
Surendra Nath Pandeya, Sivakumar Smitha, Mayank Jyoti, Seshalah Krishnan Sridhar. Biological activities of isatin and its derivatives. J Acta Pharm 2005;55:27-46.
Ayesha Zafir, Anjum Ara, Naheed Banu. In vivo antioxidant status:A putative target of antidepressant action. J Prog Neuropsychopharmacol Biol Psychiatry 2009;33:220-28.
Giselli Scaini, Brena P. Teodorak, Isabela C. Jeremias, Meline O. Morais, Francielle Mina, Diogo Dominguini et al. Antioxidant administration prevents memory impairment in an animal model of maple syrup urine disease. J Behav Brain Res 2012;231 (1):92-6.