• Mamta Sharma Faculty of Botany, Shoolini University, Solan (Himachal Pradesh) 173212, India
  • Rupinder Kaur Faculty of Botany, Uttarnchal College of Science and Technology , Dehradoon (UK) 248001, India
  • Sunil Puri Dean Academics, Shoolini University, Solan (Himachal Pradesh) 173212, India



Allelopathy, Weeds, Extract, Herbicidal effect


Objective: The aim of the study was to evaluate the bioherbicidal activity of Withania somnifera against germination and growth of noxious weeds of Himalaya i.e. Ageratum coenyzoides, Chenopodium album and Achyranthus aspera in a laboratory and foliar spray bioassay which will act as a tool of weed management in the Himalayan region.

Methods: Root, stem and leaf aqueous extract of Withania somnifera at 100% concentration were applied to determine their effect on the seed germination and seedling growth of tested weeds under laboratory condition. Foliar spray bioassay was carried out by spraying extracts of Withania somnifera on three-month-old seedlings of weeds for two months. Germination and growth of weeds were assessed using growth parameters into account i.e. germination percentage, stem length, root length, fresh weight, dry weight, no of shoot branches and no of root branches.

Results: The results revealed that all the aqueous extracts markedly suppressed germination and seedling growth of A. coenyzoides, C. album and A. aspera. Withania extracts remarkably effected germination, the growth of Ageratum coenyzoides in comparison to other studied weeds. The leaf extract of Withania was more pronounced than stem and root extracts in germination assay. Infoliarspray bioassay, aqueous leaf extracts of 100% w/v concentrations were sprayed on three month old seedlings of weeds at an interval of 5 d. All the aqueous extracts significantly suppressed shoot length, root length, fresh weight and dry weight of Ageratum coenyzoides, Chenopodium album and Achyranthus aspera seedling. In foliar spray bioassay, leaf extract suppressed the stem length, root length, fresh weight, dry weight, no. of root branches and no of shoot branches of studied weeds more commendably than stem and root extract. The suppression of growth parameters could be attributed to the presence of phytochemicals present in the extracts of Withaniasomnifera.

Conclusion: The present study concludes that Withania somnifera contain bio-herbicidal compounds in leaf, stem and root as plant extracts adversely affect weed growth of studied weeds. Henceforth the strategy bio-control of weed is a safe and effective method which could prove a milestone in weed management in Mid-Himalaya.


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Kabir M, Iqbal MZ, Shafiq M, Farooqi ZR. Effects of lead on seedling growth of Thespesia populnea. Plant Soil Environ 2010;56:194-9.

Lavin DA. The chemical defences of plants to the pathogens and herbivores. Annu Rev Ecol Evol Syst 1976;7:121-59.

Miri HR. Allelopathic potential of various plant species on Hordeum Spontaneum L. Adv Environ Biol 2011;5:3543-9.

Aldrich RJ. Weed-crop ecology, the principle in weed management. Breton Pub. North Scituate. Mass; 1984.

Baker HG. Characteristics and mode of origin of weeds. In Baker HG, Stebbins GL. The genetics of colonizing species. Academic Press: New York, USA; 1965. p. 147–72.

Baker HG. The continuing evolution of weeds. Ecol Bot 1991;45:445–9.

Holm LG, Plucknett DL, Pancho JV, Herberger JP. The World's Worst Weeds: Distribution and Biology. An East-West Centre Book University Press of Hawaii Honolulu, Hawaii; 1977. p. 609-11.

Duke JA. Promising phytomedicinal advances in new crops. Janick J, Simon JE. Editors. Timber Press Portland 1990;12:491-8.

McNeely JA. The great reshuffling: Human dimensions of invasive alien species. Ali Spec 2001;1:159-242.

Aper J. Seed germination and viability of herbicide resistant and susceptible Chenopodium. Weed Res 1998;32:219-42.

Neeta S, Uttamkumar B. Effect of Sulphaphenazole on pathogenic microorganism klebsiella aerogenes. Int J Biol 2011;2:106-10.

Kohli RK, Dogra KS, Batish DR, Singh HP. The impact of invasive plants on the structure and composition of the natural vegetation of north-western Indian Himalayas. Weed Technol 2004;18:1296-300.

Dogra KS. The impact of some invasive species on the structure and composition of the natural vegetation of Himachal Pradesh (PhD thesis). Panjab University, Chandigarh; 2008.

Zhang Z. Insights into the survival of chlamydomonas reinhardtii during sulfur starvation based on microarray analysis of gene expression. Eukaryot Cell 2004;3:1331–48.

Thapar R, Singh NB. Allelopathic effects of Amaranthus viridis on Partheniumhysterophorus. J Indian Bot Soc 2003;82:93-6.

Shafique S, Shafique S. Management of Parthenium hysterophorus L. by a natural herbicide-Tagetes erectus L. 3rd International Symposium on Weeds and Invasive Plants in Ascona, Switzerland; 2011.

Tefera T. Allelopathic effects of Parthenium hysterophorus extracts on seed germination and seedling growth of Eragrostis tef. J Agron Crop Sci 2002;188:306-10.

Rahman A. Allelopathic potential of Parthenium hysterophorus L. on Cassia sp. Allelo J 2006;18:345-55.

Chepil WS. Germination of weed seeds II. The influence of tillage treatment on germination. Sci Agric 1946b;26:347-57.

Kil B, Yun KW. Allelopathic effects of water extracts of Artemisia princeps on selected plant species. J Chem Ecol 1992;18:39-51.

Noor M, Khna MA. Allelopathic potential of Albizia samans. Pakistan J Bot 1994;26:139-47.

Shukla RS, Hussain A. Influence of various factor on toxicity of culture filtrate of a Drechslera maydis strain from Costus speciosus. J Phytopath 1987;118:187–92.

Javaid A, Anjum T, Bajwa R. Biological control of Parthenium II: Allelopathic effect of Desmostachya bipinnata on distribution and early seedling growth of Parthenium hysterophorus L. Int J Bioand Biotechnol 2005;2:459-63.

Duke SO, Dayan FE, Aliotta G, Oliva A, Romagni JG. Chemicals from nature for weed management. Weed Sci 2002;50:138-51.

Belal A, Ramady HR, Mohamed ES, Saleh AM. Drought risk assessment using remote sensing and GIS techniques. Arab J Geosci 2014;7:35-53.

Turk MA, Lee KD, Tawaha AM. Inhibitory effects of aqueous extracts of black must ard on germination and growth of radish. Res J Agric Biol Sci 2005;1:227-31.

Abdul BA, Hassan AM, Hassan AS. In vitro antimicrobial activity of Thymus vulgaris, Origanum vulgare and Rosmarinus officinalis against dental caries pathogens. J Pure Appl Sci 2012;25:1–7.

Niyati S Acharya, Jagruti J Patel. Phytochemical evaluation and in vitro antioxidant and anti-inflammatory effects of Clerodendrum serratum roots. Int J Pharm Pharm Sci 2016;8:158-63.

Krishnavignesh L, Mahalakshmipriya A, Ramesh M. In vitro analysis of phytochemical screening and antimicrobial activity of Parthenium hysterophorus l. against pathogenic microorganisms. Asian J Pharm Clin Res 2013;6:41-4.

Singh A, Uppala GK. A review on carissa carandas, phytochemistry, ethnolï€ pharmacology, and micro-propagation as conservation strategy. Asian J Pharm Clin Res 2015;8:26-30.



How to Cite

Sharma, M., R. Kaur, and S. Puri. “BIO-HERBICIDAL EFFICIENCY OF WITHANIA SOMNIFERA AGAINST IMPORTANT HIMALAYAN WEEDS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 3, Mar. 2017, pp. 88-97, doi:10.22159/ijpps.2017v9i3.14740.



Original Article(s)