INFLUENCE OF PEPTIDE P34 ON GENE EXPRESSION OF LISTERIA MONOCYTOGENES AND LISTERIA SEELEGERI

Rodrigo De Almeida Vaucher; Janice Luehring Giongo; Magton Estivales; VirgÃ­nia Cielo Rech; Vivian Shinobu Kishimoto Nishihira; Cristiane Luchese; Ethel Antunes Wilhelm; Matheus DellamÃ‰a Baldissera; Leonardo Quintana Soares Lopes; Roberto Christ Vianna Santos; Adriano Brandelli

doi:10.22159/ijpps.2017v9i1.15391

Authors

Rodrigo De Almeida Vaucher Post Graduate Program in Biochemistry and Bioprospecting, Center of Chemistry, Pharmaceutical and Food Science, Federal University of Pelotas (UFPel), Pelotas, 96010-900, Rio Grande do Sul, Brazil
Janice Luehring Giongo Laboratory of Pharmaceutical Technology, University of High Uruguay Regional Integrated (URI), Santiago, 97700-000, Rio Grande do Sul, Brazil
Magton Estivales Laboratory of Microbiological Research, Health Sciences, Franciscan University Center (UNIFRA), Santa Maria, 97010-032, Rio Grande do Sul, Brazil
VirgÃnia Cielo Rech Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
Vivian Shinobu Kishimoto Nishihira Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
Cristiane Luchese Post Graduate Program in Biochemistry and Bioprospecting, Center of Chemistry, Pharmaceutical and Food Science, Federal University of Pelotas (UFPel), Pelotas, 96010-900, Rio Grande do Sul, Brazil
Ethel Antunes Wilhelm Post Graduate Program in Biochemistry and Bioprospecting, Center of Chemistry, Pharmaceutical and Food Science, Federal University of Pelotas (UFPel), Pelotas, 96010-900, Rio Grande do Sul, Brazil
Matheus DellamÃ‰a Baldissera Microbiology and Parasitology Department, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Rio Grande do Sul, Brazil
Leonardo Quintana Soares Lopes Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
Roberto Christ Vianna Santos Microbiology and Parasitology Department, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Rio Grande do Sul, Brazil
Adriano Brandelli Laboratory of Biochemistry and Applied Microbiology, Institute of Science and Technology of Food, Federal University of Rio Grande do Sul

DOI:

https://doi.org/10.22159/ijpps.2017v9i1.15391

Keywords:

Bacteriocin, L monocytogenes, L seeligeri, Gene expression

Abstract

Objective: Investigate the influence of the antimicrobial peptides P34 and nisin on the expression of genes associated with components of the cell surface of Listeria monocytogenes and Listeria seeligeri.

Methods: Antimicrobial activity was determined by addition of peptide P34 and nisin (12.5 Âµg/ml) onto Brain Heart Infusion agar (BHI) plates previously inoculated with indicator strains (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4) after incubation for 24 h at 37 Â°C or 240 h at 4 Â°C. Ribonucleic acidÂ (RNA) was directly extracted from bacterial colonies at the border of the inhibition zones, and the expression levels of genes D-alanine-D-alanyl carrier protein ligase (dltA), putative phospholipid lysinylation (Imo 1695) and EIIAB^Man of mannose-specific PTS (mptA) were determined using real-time PCR.

Results: A non-significant increase in the levels of transcription of genes dltA, Imo1695 and mptA was observed for L. monocytogenes treated with peptide P34 or nisin. Both peptides caused a similar decrease in dltA gene expression in L. seeligeri. The expression of gene Imo1695 significantly decreased (about 2000-fold) after treatment with the peptide P34 at 37 Â°C, while at 4 Â°C a reduction of 12-fold and 5-fold was detected for P34 and nisin, respectively. A significant decrease in mptA gene expression was observed by exposition to peptide P34 (31.872-fold) and nisin (16.047-fold) for 24 h at 37 Â°C.

Conclusion: The results suggest that both peptide P34 and nisin influence the expression of genes related with the cell-surface/cell-membrane structure of L. seeligeri and in lesser extent L. monocytogenes.

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