SIMULTANEOUS DETERMINATION OF MOXIFLOXACIN HYDROCHLORIDE AND DIFLUPREDNATE BY RATIO DERIVATIVE SPECTROPHOTOMETRY

Authors

  • Ankit K. Soni Department of Pharmaceutics, Anand Pharmacy College, Anand, Gujarat. India.
  • Mukesh Gohel Department of Pharmaceutics, Anand Pharmacy College, Anand, Gujarat. India.
  • Vaishali Thakkar Department of Pharmaceutics, Anand Pharmacy College, Anand, Gujarat. India.
  • Lalji Baldaniya Department of Pharmaceutics, Anand Pharmacy College, Anand, Gujarat. India.
  • Tejal Gandhi Department of Pharmaceutics, Anand Pharmacy College, Anand, Gujarat. India.

Keywords:

Simultaneous determination, Moxifloxacin HCl, Difluprednate, Ratio derivative spectrophotometric method

Abstract

Objective: Ratio derivative spectrophotometric method has been developed for the simultaneous determination of Moxifloxacin HCl (MH) and Difluprednate (DIFLU) in Phosphate Buffer pH 7.4.

Methods: In this method, the overlapping spectra of MH and DIFLU were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of MH and DIFLU were measured at λmax 325.8 and λmax 238.0 nm, respectively for their quantification. MH and DIFLU were determined in the concentration range of 2-10 μg/ml and 4-20 μg/ml respectively.

Results: The method was validated as per the ICH guideline and accuracy, precision are found to be within the acceptable limit. The limits of detection and quantitation were found to be 0.1144 and 0.3466 μg/ml, respectively for MH and 0.0311 and 0.094 μg/ml, respectively for DIFLU.

Conclusion: The proposed ratio first derivative spectrophotometric method is novel, rapid, simple, sensitive, accurate, precise and does not require separation of MH and DIFLU hence successfully applied for simultaneous estimation of MH and DIFLU in marketed eye drops (liquid dosage form).

 

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References

Martindale. The Extra Pharmacopeia. 32th ed. The Pharmaceutical Press. London;1989.

Rang HP, Dale JP, Ritter JM, Flower RJ. Rang and Dale’s Pharmacology. 6th ed. Churchill Livingstone Elsevier;2007.

Tripathi KD. Essentials of Medical pharmacology. 6th ed. Jaypee brothers medical publishers (P) LTD;2006.

Tank DHM, Ramesh BP. Design Formulation and Evaluation of Reservoir Type Controlled Released Moxifloxacin Hydrochloride Ocular Insert. Asian J Res Pharm Sci 2013;3(1):19-24.

Formulation approval http://www.freepatents online.com. WO 2013065029 A1. Pdf.

Patel M, Singhal D, Difluprednate in Ophthalmic Practice. National J of Physiology Pharmacy & Pharmacology 2014;4(1):92-4.

Patel SK, Patel KP, Spectrophotometric estimation of loteprednol etabonate and moxifloxacin hydrochloride in eye drops by Q-absorbance ratio method. IRJP 2013.

Chauhan RS, Chabhadiya MB, Patel AK, Shah SA. Simultaneous estimation of cefixime trihydrate and moxifloxacin hydrochloride in their combined tablet dosage form by RP-HPLC. J Der Pharma Chemica 2013, 5(3):197-201.

Parmar AN, Parmar RR, Patel DVM, Shah DDA. The simultaneous estimation of moxifloxacin hydrochloride and bromfenac sodium in eye drops by UV. JPSBR 2012;1 SRC-GoogleScholar:36-9.

Dabhia MJ, Patwaria AH, Desaia UH, Doshia DB, Rathoda IS, Suhagiab BN. Simultaneous determination of moxifloxacin hydrochloride and dexamethasone sodium phosphate in eye drops by HPLC and absorbance correction method. J of Chemical and Pharm Res 2012, 4 (10):4462-67.

Patel R, Shrivastava SK, Bhandari P, Patidar A, Simultaneous estimation of moxifloxacin HCl and prednisolone acetate from eye drop formulation. Int J of Pharm & Life Sci (IJPLS) 2012;2111-4.

Sanapalal AK, Mangamma K, Anusha M, Janiki PV, Raja K. A validated RP-HPLC method for the analysis of moxifloxacin hydrochloride in pharmaceutical dosage forms. Int J of Adv in Pharm Sci2010;1 (2).

Tarkase KN, Admane SS, Sonkhede NG, Shejwal SR. Development and Validation of UV-Spectrophotometric Methods for Determination of Moxifloxacin HCL in Bulk and Pharmaceutical Formulations. J Der Pharma Chemica 2012, 4 (3):1180-5.

Yasueda SI, Masako K, Akira O, Kazuaki K, Analysis of an anti-inflammatory steroidal drug, difluprednate, in aqueous humor by combination of semi-micro HPLC and column switching method. J of Pharm and Biomedical Analysis 2003;30:1735-42.

Masazumi Y, Yasueda SI, Akiharu I, Makiko Y, Masako K, Katsuhiro I, et al. Formulation of an ophthalmic lipid emulsion containing an anti-inflammatory steroidal drug, difluprednate. Int J of Pharmaceutics 2005;301:121-8.

Indian Pharmacopoeia 2010. Vol-II. Ministry of Health & Family Welfare. J Published by Indian Pharmacopoeia Commission 2010;1700-03.

British Pharmacopoeia 2009. Vol-II. Published by J British Pharmacopoeia Commission 2009;1395-96.

Validation of analytical procedures:text and methodology, in:International Conference on Harmonization (ICH), Q2 (R1), IFPMA, Geneva, Switzerland. J Chromatogr Sci 2005.

Published

31-08-2014

How to Cite

Soni, A. K., M. Gohel, V. Thakkar, L. Baldaniya, and T. Gandhi. “SIMULTANEOUS DETERMINATION OF MOXIFLOXACIN HYDROCHLORIDE AND DIFLUPREDNATE BY RATIO DERIVATIVE SPECTROPHOTOMETRY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 387-90, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1580.

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