PHENYLPROPANOIDS AND FATTY ACIDS LEVELS IN ROOTS AND LEAVES OF DATURA STRAMONIUM AND DATURA INNOXIA
DOI:
https://doi.org/10.22159/ijpps.2017v9i7.15946Keywords:
Phenylpropanoids, Fatty acids, Datura stramonium, Datura innoxia, GC-EITOF-MSAbstract
Objective: The aim of this research was to determine and compare phenylpropanoids and fatty acids composition in two plant species, Datura innoxia and Datura stramonium.
Methods: Phenylpropanoids and fatty acids composition in leaves and roots extracted from Datura innoxia and Datura stramonium, grown under greenhouse conditions, was analyzed by gas chromatography–electron impact/time of flight-mass spectrometry (GC-EI/TOF-MS) chromatography techniques. Analyses were carried out at the Max Planck Institute for Molecular Plant Physiology of Golm (Germany).
Results: We revealed that Datura stramonium (DS) contains hydroxy-hexanedioic acid while hexanoic acid was found in Datura innoxia (DI). Also, two fatty acids are common to both Datura species, hexadecanoic acid and octadecanoic acid, with an almost equal rate between leaves and roots. However, phenylpropanoids composition revealed eight compounds; luteolin, quercetin, trans-caffeic acid, trans-ferulic acid, cis-caffeic acid, cis-4-hydroxy-cinnamic acid, trans-4-hydroxy-cinnamic acid and trans-sinapic acid in DI. However, in DS, five compounds were detected: luteolin, quercetin, trans-caffeic acid, trans-ferulic acid and dihydroferulic acid. Also in both Datura species, phenylpropanoids concentration in leaves was significantly higher than in the roots.
Conclusion: Our results showed a difference in phenylpropanoids and fatty acids compositions between the two Datura species, with a significantly higher concentration of phenylpropanoids in Datura innoxia than in Datura stramonium
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References
Drake LR, Lin S, Rayson GD, Jackson PJ. Chemical modification and metal binding studies of Datura innoxia. Environ Sci Technol 1995;30:110-4.
Griffin WJ, Lin GD. Chemotaxonomy and geographical distribution of tropane alkaloids. Phytochemistry 2000;53:623–37.
Hopkins WG. Physiologie végétale. De Boeck Supérieur; 2003.
Hahlbrock K, Scheel D. Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Biol 1989; 40:347-69.
Weisshaar B, Jenkins GI. Phenylpropanoid biosynthesis and its regulation. Curr Opin Plant Biol 1998;1:251-7.
Máthé Ã. Medicinal and Aromatic Plants of the World. Springer; 2015.
Seigler DS. Plant secondary metabolism. Springer Science and Business Media; 2012.
Browse J, Somerville C. Glycerolipid synthesis: biochemistry and regulation. Annu Rev Plant Biol 1991;42:467-506.
Witte L, Müller K, Arfmann HA. Investigation of the alkaloid pattern of Datura innoxia plants by capillary gas-liquid-chromatography-mass spectrometry. Planta Med 1987;53:192-7.
Ionkova I, Witte L, Alfermann AW. Spectrum of tropane alkaloids in transformed roots of Datura innoxia and Hyoscyamus cultivated in vitro. Planta Med 1994;60:382-4.
Erban A, Schauer N, Fernie AR, Kopka J. Nonsupervised construction and application of mass spectral and retention time index libraries from time-of-flight gas chromatography-mass spectrometry metabolite profiles. Metabol Methods Protocols 2007;358:19-38.
Watson JT, Schultz GA, Tecklenburg RE, Allison J. Renaissance of gas chromatography-time-of-flight mass spectrometry: meeting the challenge of capillary columns with a beam deflection instrument and time array detection. J Chromatogr 1990;518:283-95.
Veriotti T, Sacks R. High-speed GC and GC/time-of-flight MS of lemon and lime oil samples. Anal Chem 2001;73:4395-402.
Khelifi-Slaoui M, Rezine R, Amroun S, Khelifi L. Embryons somatiques et bourgeons néoformés induitsur explants issus de vitrosemis de Datura stramonium L. d’origine algérienne. Actes du séminaire international sur l’amélioration des productions; 2005.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962;15:473-97.
Wagner C, Sefkow M, Kopka J. Construction and application of a mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles. Phytochemistry 2003;62:887-900.
Pant BD, Pant P, Erban A, Huhman D, Kopka J, SCHEIBLE WR. Identification of primary and secondary metabolites with phosphorus status-dependent abundance in arabidopsis, and of the transcription factor PHR1 as a major regulator of metabolic changes during phosphorus limitation. Plant Cell Environ 2015;38:172-87.
Lin Y, Shi R, Wang X, Shen HM. Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 2008;8:634-46.
Srinivasan M, Sudheer AR, Menon VP. Ferulic acid: therapeutic potential through its antioxidant property. J Clin Biochem Nutr 2007;40:92-100.
Boerjan W, Ralph J, Baucher M. Lignin biosynthesis. Annu Rev Plant Biol 2003;54:519-46.
Gunstone FD, Harwood JL, Dijkstra AJ. The lipid handbook with CD-ROM. CRC Press; 2007.
Ichikawa M, Ryu K, Yoshida J, Ide N, Kodera Y, Sasaoka T, et al. Identification of six phenylpropanoids from garlic skin as major antioxidants. J Agric Food Chem 2003;51:7313-7.
Kim S, Ju E, Hossain A, Lee H, Kim H. Global profiling of ultraviolet-induced metabolic disruption in Melissa officinalis by using gas chromatography-mass spectrometry. Anal Bioanal Chem 2012;404:553-62.
Menon DB, Sasikumar JM. Pharmacognostic study and phytochemical investigation of Plectranthus hadiensis. Int J Pharm Pharm Sci 2011;3:300-4.
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