CHEMICAL COMPOUNDS AND BIOLOGICAL ACTIVITY OF AN EXTRACT FROM BOUGAINVILLEA X BUTTIANA (VAR. ROSE) HOLTTUM AND STANDL
Keywords:Anti-inflammatory, Antioxidant, Phenolic compound
Objective: A Bougainvillea x buttiana (var. Rose) Holttum and Standl extract (BxbREE) was prepared and its chemical composition, antioxidant and anti-inflammatory activity were evaluated.
Methods: For the analyses of the phytochemical compounds present in BxbREE extract, gas chromatography-mass spectrometry (GC/MS) was used. To explore the anti-oxidant, anti-inflammatory activities, total phenolic contents, carbohydrates, lipids and carrageenan-induce paw edema models, respectively, were used. For in vivo experiments, the extract was orally, intraperitoneally and/or subcutaneously administered at doses of 0.04, 0.4, 4 and 40 mg/kg.
Results: GC/MS analyses showed the presence of 7 compounds, including 2-Propenoic acid, 3-(2-hydroxyphenyl)-, (E)-(1.19%); 2-Methoxy-4-vinylphenol (0.22%); 3-O-Methyl-d-glucose (92.14%); n-Hexadecanoic acid (0.76%); Hexadecanoic acid, ethyl ester (1.17%); 9,12-octadecadienoic acid, ethyl ester (1.93%); and 9,12,15-Octadecatrienoic acid, ethyl ester (Z,Z,Z) (2.59%). Phytochemical qualitative analysis showed the presence of total phenolic contents at 320 mg of Gallic acid Equivalent/gram of dried extract (GA-Eq/g extract); carbohydrates 5.18 mg/ml and lipids 13.88 mg/ml. In accordance the structures the major compound was 3-O-Methyl-d-glucose. Our results also clearly indicate that BxbREE decreases inflammation in BALB/c mice as a subplantar injection of carrageenan-induced paw edema. The extract presented a potent dose-dependent inhibitory effect. The edema inhibition percentage was significantly lower in groups of animals treated with BxbREE by via intraperitoneal or subcutaneous when compared with those results obtained for groups treated by orally administration (p<0.001).
Conclusion: In conclusion, this study established the anti-oxidant and anti-inflammatory activities of Bougainvillea x buttiana (var. Rose); also, this extract could be considered to be a natural anti-oxidant agent that represents an anti-inflammatory remedy.
Cos P, Vlietinck AJ, Berghe DV, Maes L. Anti-infective potential of natural products: how to develop a stronger in vitro â€˜proof-of-conceptâ€™. J Ethnopharmacol 2006;106:290-302.
Milne A. Inhalational and local anesthetics reduce tactile and thermal responses in Mimosa pudica linn. Masui 1993;2:1190-3.
Balasaraswathi R, Sadasivam S, Ward M, Walker JM. An antiviral protein from Bougainvillea spectabilis roots; purification and characterization. Phytochemistry 1998; 47:1561-5.
Pun KB, Sabitha D, Jeyarajan R. Screening of plants species for the presence of antiviral principles against okra yellow vein mosaic virus. Indian J Phytopathol 1998;52:221-3.
Joshi DD, Mujumdar AM, Narayanan CR. Anti-inflammatory activity of Bougainvillea spectabilis leaves. Indian J Pharm Sci 1998;46:187-8.
Davidson MB, Peters AL, Schringer DS. An alternative approach to the diagnosis of diabetes with a review of the literature. Diabetes Care 1998;8:1065-71.
Mishra N, Joshi S, Tandon VL, Munjal A. Evaluation of antifertility potential of Bougainvillea spectabilis leaves in swiss albino mice. Int J Pharm Sci Drug Res 1998;1:19-23.
Alvarez Perez Gil AL, Barbosa Navarro L, Patipo Vera M, Petricevich VL. Anti-inflammatory and aninocieptive activites of the ethanolic extract of Bougainvillea xbuttiana. J Ethnopharmacol 2012;144:712-9.
Arteaga Figueroa L, Barbosa Navarro L, PatiÃ±o Vera M, Petricevich VL. Antioxidant activity, total phenolic and flavonoid contents, and cytotoxicity evaluation of Bougainvillea xbuttiana. Int J Pharm Pharm Sci 2014;6:498-502.
Dominguez XA. MÃ©todos de InvestigaciÃ³n FitoquÃmica, Limusa, Mexico City, MÃ©xico; 1985.
Aziz MA. Qualitative phytochemical screening and evaluation of anti-inflammatory, analgesic and antipyretic activities of Microcos paniculata barks and fruits. J Integr Med 2015;13:173-84.
Mishra SK, Suh WI, Farooq W, Moon M, Shrivastav A, Park MS, et al. Rapid quantification of microalgal lipids in an aqueous medium by a simple colorimetric method. Bioresour Technol 2014;155:330â€“3.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol 1999;299:152-78.
OECD. Guidance document on the recognition, assessment and use of clinical signs as humane endpoints for experimental animals used in safety evaluation. Environmental Health and Safety Monograph Series on Testing and Assessment N Â° 19, ENV/JM/MONO; 2000.
Olufunmilayo O, Adeyemi SO, Okpo OO. The analgesic effect of methanolic extract of Acanthus montanus. J Ethnopharmacol 2004;90:45-68.
Winter CA, Risley EA, Nuss CW. Carrageenan-induced oedema in hind paw of the rat as an assay for anti-inï¬‚ammatory drugs. Proc Soc Exp Biol Med 1962;111:544â€“7.
Mujumdar AM, Misar AV. Anti-inflammatory activity of Jatropha curcas roots in mice and rats. J Ethnopharmacol 2004;90:11-5.
Ponnamma SU, Manjunath K. GC-MS analysis of phytocomponents in the methanolic extract of Justicia wyaadensis (NEES) T. Anders. Int J Pharma Bio Sci 2012;3:570-6.
Jin BJ, Se CH, Hyung JJ, Jin SK. Anti-inflammatory effect of 2-methoxy-4-vinylphenol via the suppression of NF-ÎºB and MAPK activation, and acetylation of histone H3. Arch Pharmacol Res 2011;34:2109â€“16.
Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italic leaf methanol extract. Asian J Pharm Clin Res 2012;5:90-4.
Jananie RK, Prya V, Vijayalakshmi K. Determination of bioactive components of Cynodon dactylon by GC-MS analysis. New York Sci J 2011;4:16-20.
Frydoonfar HR, McGrath DR, Spigelman AD. The variable effect on proliferation of a colon cancer cell line by the citrus fruit flavonoid naringenin. Colorectal Disease 2003;5:149-52.
Hakimuddin F, Paliyath G, Meckling K. Selective cytotoxicity of a red grape wine flavonoid fraction against MCF-7 cells. Breast Cancer Res Treat 2004;85:65-79.
Reddy KS, Katan MB. Diet, nutrition and the prevention of hypertension and cardiovascular diseases. Public Health Nutr 2004;7(1a):167-86.
QueirÃ³z RF, JordÃ£o AK, Cunha AC. Nitroxides attenuate carrageenan-induced inflammation in rat paws by reducing neutrophil infiltration and the resulting myeloperoxidase-mediated damage. Free Radical Biol Med 2012;53:1942â€“53.
Huang WY, Cai YZ, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer 2010;62:1-20.
Kumar V, Abbas AK, Fausto N, Mitchell RN. Acute and Chronic Inflammation. In: Robbins Basic Pathology: Eighth Edition Saunders Elsevier. Philadelphia; 2007. p. 31-2.
Flower RJ, Perretti M. Controlling inflammation: a fat chance? J Exp Med 2005;201:671-4.