• Dilipkumar Pal Department of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh, 495009, India
  • Vipul Singh KNIMT Faculty of Pharmacy, Sultanpur, UP, 218128, India
  • Desh Deepak Pandey KNIMT Faculty of Pharmacy, Sultanpur, UP, 218128, India
  • Roopesh Kumar Maurya KNIMT Faculty of Pharmacy, Sultanpur, UP, 218128, India


Antibacterial activity, Antifungal activity, 1, 2, 4-Triazole


Objectives: To synthesize, characterize and evaluate antimicrobial properties of some 1, 2, 4-triazole derivatives.

Methods: A novel series of 1, 2, 4-Triazole derivatives (D-1-D-8) had been synthesized. Ethyl esters of benzoic and 4-substituted benzoic acids were synthesized using ethanol and conc. sulphuric acid. In the second step, hydrazides of these esters were prepared. This hydrazide was converted into potassium salt of dithiocarbazinate using carbon disulfide and potassium hydroxide which on cyclization formed compounds (D-1-D-2). Compound D-3 was formed by reacting D-1 with 4-methylbenzenesulfonyl chloride in dry pyridine. Compounds (D-4-D-8) were synthesized by mixing aqueous solution of 10% NaOH in different primary amines and then heating it with potassium salt of dithiocarbazinate. The structures of newly synthesized compounds were established on the basis of 1H NMR and Mass spectroscopic techniques. The newly synthesized compounds were screened for their in vitro antibacterial and antifungal activity. In vitro antibacterial and antifungal activity was evaluated by Disc Diffusion method. Ofloxacin and Clotrimazole were used as standard drug respectively.

Results: The results revealed that compounds D-3 and D-4 exhibited good antibacterial activity and D-1 and D-2 had moderate antibacterial activity as compared with standard drug Ofloxacin, while compounds (D-5-D-8) exhibited moderate antifungal activity as compared to standard drug Clotrimazole.

Conclusion: A novel series of 1, 2, 4-Triazole derivatives were synthesized and were obtained in good yields. Newly synthesized compounds were isolated and purified by thin layer chromatography and column chromatography respectively.


Download data is not yet available.

Author Biography

Dilipkumar Pal, Department of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh, 495009, India

Associate Professor

Department of Pharmaceutical Sciences, Guru Ghasidash Vishwavidyalya

(A Central University)


Chanda S, Baravalia Y, Baluja S. Synthesis and antibacterial activity of some new triazole derivatives. J Archives of Appl Sci Res 2010;2 (3):117-26.

Colanceska-Ragenovic K, Dimova V, Kakurinov V, Molnar Dora G, Buzarovska A. Synthesis, Antibacterial and Antifungal Activity of 4-Substituted-5-Aryl-1, 2, 4-Triazoles. J Molecules 2001;6:815-24.

Lazarevic M, Dimova V, Molnar GD, Kakurinov V, Colanceska K. Synthesis of some N1–aryl/heteroarylaminomethyl/ethyl–1, 2, 4–triazoles and their Antibacterial and antifungal activities. J Heterocyclic Comm 2001;7 (6):577-82.

Nizami Syed A, Gurumurthy M, Chattarjee SJ, Panda D. Evaluation of Antimicrobial Potency of Some Synthesized Thiazolidin-4-one substituted 1, 2, 4-triazoles. J Adv Pharm Res 2010;1:26-35.

Bhat AR, Bhat GV, Shenoy GG. Synthesis and in-vitro antimicrobial activity of new 1, 2, 4-triazoles. J Pharm Pharmacol 2010;53(2):267-72.

Lingappa B, Girisha KS, Balakrishna K, Satheesh NR, Nalilu SK. Novel Mannich bases derived from 3-(4,6-disubstituted-2-thiomethyl)-3-amino-5-mercapto-1, 2, 4-triazoles as a potent antifungal agent. Indian J Chem. 2008;47(B):1858-64.

Patel Navin B, Khan Imran H. Synthesis of 1, 2, 4-triazole derivatives containig benzothiazoles as pharmacologically active molecule. J Enz Inhib and Med Chem 2011;26(4):527-34.

Husain MI, Amir M., Singh E. Synthesis and anti-tubercular activities of [5-(2 furyl)-1, 2, 4-triazoles-3yl thio] acehydrazide derivatives. Indian J Chem 1987;26B:2512-54.

Sunil D, M Isloor A, Shetty P. Synthesis, characterization and anticancer activity of 1, 2, 4-Triazolo [3, 4-b]-1, 3, 4-thiadiazoles on Hep G2 cell lines. J Der Pharma Chemica 2009;1(2):19-20.

El-Sayed WA, Flefel Eman M, Morsy Eman MH. Anticancer and antimicrobial activities of some synthesized pyrazole and triazole derivatives. J Der Pharma Chemica 2012;4 (1):23.

Pal D, Banerjee S, Ghosh A. Dietary Induced Cancer Prevention:An Expanding Research Arena of Emerging Diet, related to health care system. J Adv Pharm Tech Res 2012;3(1):16-24.

Al-Soud YA, Al-Masoudi NA, Ferwanah Ael-R. Synthesis and properties of new substituted 1, 2, 4-triazoles:potential antitumor agents. J Bioorg Med Chem 2003;11:1708.

Goyal Pradeep K, Bhandari A, Rana AC, jain CB. Synthesis of Some 3-Substituted-4H-1, 2, 4-Triazole Derivatives with Potent Anti-Inflammatory Activity. Asian J Pharm and Clin Res 2010;3(3):244-46.

Sambrekar Sudhir N, Suhas AP. Synthesis of 3-Substituted-4-Amino-5-Mercapto-4(H)-1, 2, 4-Triazole and Screening for Anti-Convulsant Activity. Int J Res in Pharm and Biomed Sci 2011;2 (2):520-24.

Kane MJ, Dudley MW, Sorensen MS, Miller FP. Synthesis of 2, 4-Dihydro-3H-1, 2, 4-triazole-3-thiones as potential antidepressant agents. J Med Chem 1988;31:1253-58.

Khanage S, Mohite Popat B, Pandhare Ramdas B, Raju SA. Study of analgesic activity of novel 1, 2, 4-triazole derivatives bearing pyrazole and tetrazole moiety. J Pharm Res 2011;4(10):609-11.

Farghalya Abdel-R, Clercq Erik D, El-Kashef H. Synthesis and antiviral activity of novel [1,2,4] triazolo [3,4-b] [1,3,4] thiadiazoles,[1,2,4] triazolo [3,4-b] [1,3,4] thiadiazines and [1,2,4] triazolo [3,4-b] [1,3,4] thiadiazepines. J Arkivoc 2006;10:137-51.

Boechat N, Pinheiro Liiz CS, Santos-Filho Osvaldo A, Silva Isabor C. Design and Synthesis of New N-(5-Trifluoromethyl)-1H-1, 2, 4-triazol-3-yl Benzenesulfonamides as Possible Antimalarial Prototypes. J Molecule 2011;16:8083-97.

Mhasalkar MY, Shah MH, Nikam ST. Further studies in substituted 4H-1, 2, 4-Triazoles for Possible Hypoglycemic Activity. J Med Chem 1971;14(3):260-62.

Deliwala CV, Mhasalkar MY, Shaj MH, Pilankar PD, Nikam ST, Anantanarayan KG. Synthesis and Hypoglycaemic activity of 3-aryl (or pyridyl)-5-alkyl amino-1, 3, 4 Thiadiazole and some sulfonyl ureas derivatives of 4H-1, 2, 4 triazoles. J Med Chem 1971;14(10):1000-03.

Pal D, Mitra S. A preliminary study on the in vitro antioxidant activity of the stems of O. vulgaris. J Adv Pharm Tech Res 2010;1(2):268-72.

Singhal N, Sharma PK, Dudhe R, Kumar N. Recent advancement of triazole derivatives and their biological significance. J Chem Pharm Res 2011;3(2):126-27.

Nayak AK, Pal D, Pany DP, Mohanty B. Evaluation of S. oleracea L mucilage as an innovative suspending agent. J Adv Pharm Tech Res 2010;1(3):338-41.



How to Cite

Pal, D., V. Singh, D. D. Pandey, and R. K. Maurya. “SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL EVALUATION OF SOME 1, 2, 4-TRIAZOLE DERIVATIVES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 213-6,



Original Article(s)