• Rabab Omran Biology Department, College of Science, University of Babylon, Babil, Al-Hillah, Iraq



Nocardiopsis, Keratinase, Antimicrobial, Anticancer, Feather medium


Objective: Production of bioactive secondary metabolites from a feather-degrading actinobacterial species using feather meal medium.

Methods: Protease producer actinobacterial isolates (22) recovered from farm soil, poultry farm soil and feather wastes were used to test the antagonistic effect on pathogenic bacteria and fungi, including Staphylococcus aureus, Escherichia coli, Microsporum canis and Trichophyton mentagrophyte using Mueller-Hinton agar and potato dextrose agar (PDA) media. The best isolate was used to produce the active metabolites in feather meal medium composed from (g/l) 10 feather meal, 5 sucrose and 0.3 l cement extract were dissolved in tap water, in addition to the standard medium composting of (g/l) glucose (1%), tryptone (1%), KH2PO4 (0.07%) and K2HPO4 (0.14%) were dissolved in distilled water, both of them at initial pH 9. The secondary metabolites were partially purified by Sephadex LH20 column and the antimicrobial activity and cytotoxic activity were assayed.

Results: 31.82% of isolates inhibited the growth of both bacterial and fungal test organisms and the best one was Nocardiopsis sp. 28ROR (GenBank: KC702802.1) at a significant level PË‚ 0.05. It produced bioactive metabolites in both feather meal broth and the standard media. The partially purified metabolites inhibit the breast cancer cell line MCF-7 (51%) and normal hepatic cell line WRL-68 (9%), in addition to inhibiting S. aureus andTrichophyton menta agrophyte growth.

Conclusion: The actinobacteria has vast abilities to degrade very complex wastes and converted to simple constituents to reprocess in other industries. So the Nocardiopsis sp. 28ROR was a novel strain produced anticancer, antimicrobial substances using feather meal medium as a cheap waste medium.


Download data is not yet available.


Bennur T, Kumar AR, Zinjarde S, Javdekar V. Nocardiopsis species as potential sources of diverse and novel extracellular enzymes. Appl Microbiol Biotechnol 2014;98:9173–85.

Sun HH, White CB, Dedinas J, Cooper R, Sedlock DM. Methylpendolmycin, an Indo lactam from a Nocardiopsis sp. J Nat Prod 1991;54:1440–3.

Ding ZG, Li MG, Zhao JY, Ren J, Huang R, Xie MJ, et al. Naphthospironone a: an unprecedented and highly functionalized polycyclic metabolite from an alkaline mine waste extremophile. Chem Eur J 2010;16:3902–5.

Kim JW, Adachi H, Shin-ya K, Hayakawa Y, Seto H. Apoptolidin, a new apoptosis inducer in transformed cells from Nocardiopsis sp. J Antibiot 1997;50:628–30.

Li YQ, Li MG, Li W, Zhao JY, Ding ZG, Cui XL, et al. Griseusin D, a new pyranonaphthoquinone derivative from a alkaphilic Nocardiopsis sp. J Antibiot 2007;60:757–61.

Engelhardt K, Degnes KF, Kemmler M, Bredholt H, Fjaervik E, Klinkenberg G, et al. Production of a new thiopeptide antibiotic TP-1161, by a marine Nocardiopsis species. Appl Environ Microbiol 2010;76:4969–76.

Gandhimathi R, Seghal KG, Hema TA, Selvin J, Rajeetha RT, Shanmughapriya S. Production and characterization of lipopeptide biosurfactant by a sponge associated marine actinomycetes Nocardiopsis alba MSA10. Bioprocess Biosyst Eng 2009;32:825–35.

Kanchana R. Farm waste recycling through microbial keratinases. J Appl Sci Environ Sanit 2012;7:103–8.

Gopinath SCB, Anbu P, Lakshmipriya T, Tang TH, Chen Y, Hashim U. Biotechnological aspects and perspective of microbial keratinase production. BioMed Res Int 2015.

Gupta R, Ramnani P. Microbial keratinases and their prospective applications: an overview. Appl Microbiol Biotechnol 2006;70:21–33.

Saibabu V, Niyonzima FN, More SS. Isolation, partial purification and characterization of keratinase from Bacillus megaterium. Int Res J Biol Sci 2013;2:13-20.

Madigan MT, Martinko JM, Stahl DA, Clark DP. Commercial products and biotechnology. In: Brock biology of microorganisms. 13th ed. Boston: Benjamin cumings; 2012. p. 415-6.

Crawford DL, Lynch JM, Whipps JM, Ousley MA. Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl Environ Microbiol 1993;59:3899–905.

Perez C, Pauli M, Bazevque P. An antibiotic assay by the agar-well diffusion method. Acta Biol Med Exp 1990;15:113-5.

Omran R, Kadhem MF. Production, purification, and characterization of bioactive metabolites produced from rare actinobacteria Pseudonocardia alni. Asian J Pharm Clin Res 2016;9(Suppl 3):1-9.

Wawrzkiewicz K, Lobarzewski J, Wolski T. Intracellular keratinase of trichophyton gallinae. J Med Vet Mycol 1987;25:261–8.

Gel filtration: principles and methods. 7th ed. Pharmacia Biotech; 1997.

Freshney RI. The culture of an animal cell. 6 ed. Wily-Liss, New York; 2010.

Chih PL, Wei JT, Yuang LL, Yuh CK. The extracts from nelumbonucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells. Life Sci 2004;75:699-16.

Singh C, Parmar RS, Jadon P, Kumar A. Characterization of actinomycetes against phytopathogenic fungi of glycine max (L.). Asian J Pharm Clin Res 2016;9(Suppl 1):216-9.

Nanjwade BK, Chandrashekhara S, Goudanavar PS, Shamarez AM, Manvi FV. Production of antibiotics from soil-isolated actinomycetes and evaluation of their antimicrobial activities. Trop J Pharm Res 2010;9:373-7.

Kalyani ALT, Ramya Sravani KM, Annapurna J. Isolation and characterization of antibiotic producing actinomycetes from marine soil samples. Int J Curr Pharm Res 2012;4:109-12.

Pandey A, Ali I, Butola KS, Chatterji T, Singh V. Isolation and characterization of actinomycetes from soil and evaluation of antibacterial activities of actinomycetes against pathogens. Int J Appl Biol Pharm Technol 2011;2:384-92.

Kumari M, Myagmarjav BE, Prasad B, Choudhary M. Identification and characterization of antibiotic-producing actinomycetes isolates. Am J Microbiol 2013;4:24-31.

Harrap BS, Woods EF. Soluble derivatives of feather keratin. 1. Isolation, fractionation and amino acid composition. Biochem J 1964;92:8-18.

Bishop M, Bott SG, Barron AR. A new mechanism for cement hydration inhibition: solid-state chemistry of calcium nitrilotris (methylene) triphosphonate. Chem Mater 2003; 15:3074-88.

Oskay M. Isolation and purification of two metabolites (KGG32-A and KGG32-B) from a soil bacterium, Streptomyces sp., KGG32. Int J Agric Biol 2011;13:369–74.

Lorian V. Antibiotics in laboratory medicine. Lippincott Williams and Wilkins. USA; 2005. p. 654.

Zhang C, Li S, Tian X, Niu S, Zhang W, Chen Y, et al. Pseudonocardians A–C, new diaza anthraquinone derivatives from a deep-sea actinomycete Pseudonocardiasp. SCSI 01299. Mar Drugs 2011;9:1428-39.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 y. J Nat Prod 2007;70:461-77.

Bérdy J. Bioactive microbial metabolites. J Antibiot 2005;58:1–26.

Jeena K, Liju VB, Kuttan R. Antitumor and cytotoxic activity of ginger essential oil (Zingiber officinale roscoe). Int J Pharm Pharm Sci 2015;7:341-4.

Herdwiani W, Soemardji AA, Elfahmi I, Tan MI. A review of cinnamon as a potent anticancer drug. Asian J Pharm Clin Res 2016;9:8-13.



How to Cite

Omran, R. “PRODUCTION OF ANTIMICROBIAL AND ANTICANCER FOM FEATHER-KERATINOLYTIC NOCARDIOPSIS SP. 28ROR AS A NOVEL STRAIN USING FEATHER MEAL MEDIUM”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 3, Mar. 2017, pp. 175-9, doi:10.22159/ijpps.2017v9i3.16426.



Original Article(s)