EFFECT OF BACOSIDE A ON LIPID PEROXIDATION IN D-GALACTOSE INDUCED AGING MICE
Keywords:Oxidative stress, Aging, Lipid peroxidation, D-galactose, Bacoside A, Antioxidant
Objective: Bacoside A is a major bioactive constituent of Bacopa monnieri L having antioxidant property. The objective of this study was to evaluate the effect of Bacoside A, on lipid peroxidation in brain, heart and liver during induced aging.
Methods: Male Swiss albino mice, Mus musculus was used for the present investigation. Four experimental groups were used as Group I-Normal adult, Group II-D-galactose induced, Group III-D-galactose induced plus Bacoside A treated and Group IV-Natural aging. The effect of Bacoside A was studied against lipid peroxidation during induced aging. The level of lipid peroxidation in the form of MDA formation was determined and measured in brain, heart and liver.
Results: The statistical data obtained were analyzed using one way ANOVA, control vs other groups and results were expressed as meanÂ±SE. In Bacoside A treated group the lipid peroxidation level in heart, brain and liver was significantly decreased (p<0.001) compared to control group. A significant increase (p<0.0001) in the level of lipid peroxidation was observed in D-galactose induced mice. In natural aging group highly significant increase (p<0.0001) in initial lipid peroxidation, ascorbate dependent lipid peroxidation and spontaneous lipid peroxidation was observed.
Conclusion: The observations revealed that, lipid peroxidation was reversed in Bacoside A treated group which may be due to antioxidant property of Bacoside A. Thus Bacoside A is able to ameliorate the stress induced changes in lipid peroxidation during aging. The findings also provide a theoretical basis for the development of novel therapeutic formulations, such as antioxidant supplementation to boost antioxidant defenses in the body.
Sister TF. Free radical mechanism in tissue injury. Biochem J 1984;222:1-15.
Halliwell B, Gutteridge JM. The importance of free radicals and catalytic metal ions in human diseases. Mol Aspects Med 1985;8:89-193.
Sevanian A, Hochstein P. Mechanisms and consequences of lipid peroxidation in biological systems. Annu Rev Nutr 1985;5:365-90.
Harman D. Prolongation of life: Role of free radical reactions in aging. J Am Geriat Soc 1969;17:721-35.
Tappel AL. Will antioxidant nutrients slow aging processes? Geriatrics 1968;23:97-105.
Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993;90:7915â€“22.
McCord JM. Human disease, free radicals, and the oxidant/antioxidant balance. Clin Biochem 1993;26:351-7.
Patro IK. Lipofuscinolysis by four neurotropic agents: a comparative study In: Perspectives in aging research, Biological, medical and Social. eds. R Singh. Today and tomorrowâ€™s Printers and Publishers, New Delhi; 1990. p. 133-6.
Brunk UT, Jones CB, Sohal RS. A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. Mutat Res 1992;275:395-403.
Yu BP. In: BP Yu. Ed. Free radicals in aging, CRC Press; Boca Raton: 1993. p. 57â€“88.
Vora SR, Patil RB, Pillai MM. Protective effects of Petroselinum crispum (Mill) Nyman ex A. W. Hill leaf extract on D-galactose-induced oxidative stress in mouse brain. Indian J Exp Biol 2009;47:338-42.
Munch G, Simm A, Double KL, Reiderer P. Alzheimerâ€™s Disease Review; 1996. p. 71-4.
Schmidt AM, Hori O, Cao R, Yan SD, Brett J, Wautier JL, et al. Diabetic dyslipidemia: basic mechanisms underlying the common hypertriglyceridemia and low HDL cholesterol levels. Diabetes 1996;45(Suppl 3):S27-30.
Hamada Y, Araki N, Kou N, Nakamura J, Horiuchi S, Hotta N. Rapid formation of advanced glycation end products by intermediate metabolites of the glycolytic pathway and polyol pathway. Biochem Biophys Res Commun 1996;228:539.
Khan BA, Abraham A, Leelamma S. Role of Murraya koenigii (Curry leaf) and Brassica Juncea (Mustard) in lipid peroxidation. Indian J Physiol Pharmacol 1996;40:155-8.
Chithra V, Leelamma S. Coriandrum sativum changes the levels of lipid peroxides and activity of antioxidant enzymes in experimental animals. Indian J Biochem Biophys 1999;36:59-61.
Zhang Q, Ning L, Zhou G, Lu X, Xu Z, Li Z. In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice. Pharmacol Res 2003;48:151-5.
Fidan AF, Cingi CC, Karafakioglu YS, Utuk AE, Pekkaya S, Piskin FC. The levels of antioxidant activity, malondialdehyde and nitric oxide in cows naturally infected with Neospora caninum. J Anim Vet Adv 2010;9:1707-11.
Alagumanivasagam G, Muthu AK, Manavalan R. Antioxidant and lipid peroxidation effect of methanolic extract of the whole plant of Teramnus labialis (Linn.) in rat fed with high-fat diet. Int J Pharm Tech Res 2012;4:1233-7.
Jasuja N, Sharma P, Joshi SC. Ameliorating effect of Withania somnifera on acephate administered male albino rats. Afr J Pharm Pharmacol 2013;7:1554-9.
Kotebagilu NP, Palvai VR, Urooj A. Protective effect of selected medicinal plants against Hydrogen peroxide induced oxidative damage on biological substrates. Int J Med Chem 2014;1-7. http://dx.doi.org/10.1155/2014/861084
Porchelvan V, Venkatakrishnamurali R. The Aegle marmelos leaf extracts and whole leaf powder influencing effects on experimental animals tissue antioxidants and Atpases during chronic administration. Asian J Phytomed Clin Res 2015;3:13-23.
Nadkarni KM. Indian Materia Medica. Popular Prakashan Pvt Ltd. Mumbai; 1976. p. 624-5.
Singh HK, Rastogi RP, Srimal RC, Dhawan BN. Effects of bacoside A and B on avoidance response in rats. Phytother Res 1988;2:70-4.
Tripathi YB, Chaurasia S, Tripathi E, Upadyay A, Dubey GP. Bacopa monniera Linn. As an antioxidant: mechanism of action. Indian J Exp Biol 1996;34:523-6.
Singh HK, Dhawan BN. Neuropsychopharmacological effects of the Ayurvedic nootropic Bacopa monniera Linn. (Brahmi). Indian J Pharmacol 1997;29:S359-65.
Anbarasi K, Vani G, Balakrishna K, Shyamala Devi CS. Creatine kinase isoenzyme patterns upon chronic exposure to cigarette smoke: Protective effect of Bacoside A. Vascul Pharmacol 2005;42:57-61.
Pawar SS, Jadhav MG, Deokar TG. Study of phytochemical screening, physicochemical analysis and antimicrobial activity of Bacopa monnieri (L) extracts. Int J Pharm Clin Res 2016;8:1222-9.
Pawar SS, Jadhav MG. Determination and quantification of bacoside A from Bacopa monnieri L. by high-performance thin layer chromatography. Int J Pharm Phytopharm Res 2015;7:1060-5.
Garai S, Mahato SB, Obtani K, Yamasaki K. Dammarane type triterpenoid saponins from Bacopa monniera. Phytochemicals 1996;42:815-20.
Packer L, Deamer DW, Heath RL. Regulation and deterioration of structure in membranes. Adv Gerontol Res 1967;2:77.
Tappel AL. Biological antioxidant protection against lipid peroxidation damage. Am J Clin Nutr 1970;23:1137-9.
Koudelova J, Mourek J. The lipid peroxidation in various parts of the rat brain: effect of age, hypoxia and hyperoxia. Physiol Res 1994;43:169-73.
Pawar SS. Age related changes in brain acid phosphatase. Thesis Submitted to Shivaji University, Kolhapur; 2002.
Ho SC, Liu JH, Wu RY. Establishment of mimetic aging effect in mice caused by D-galactose. Biogerontology 2003;4:15-8.
Song X, Bao M, Li D, Li YM. Advanced glycation in D-galactose induced mouse aging model. Mech Aging Dev 1999;108:239-51.
Ida H, Ishibashi K, Reiser K, Hjelmeland LM, Handa JT. Ultrastructural aging of the RPE Bruch's membraneâ€“choriocapillaris complex in the D-galactose-treated mouse. Investigative Ophthalmol Visual Sci 2004;45:2348-54.
Deshmukh AA, Gajare KA, Pillai MM. D-galactose induced aging in short duration: a quick model of accelerated ageing in mice. J Cell Tissue Res 2006;6:753-6.
Deshmukh AA, Gajare KA, Pillai MM. Six-month-old mice show increased lipid peroxidation and increased antioxidant enzymes with fifteen days treatment of 5% D galactose: a quick model of oxidative stress for research on aging. Proc Comp Animal Physiol Stress Physiol. Osmania University, Hydrabad; 2005. p. 28.
Gajare KA, Deshmukh AA, Pillai MM. Protective effects of Bacopa monniera in D galactose induced oxidative stress in brain of female albino mice. Proc Comp Animal Physiol Stress Physiol. Osmania University, Hydrabad; 2005. p. 16.